The disclosure provides for provides for water-soluble near-infra red (NIR) emissive compounds, processes of making the compounds thereof, and use of the compounds therein.

The disclosure provides for provides for water-soluble near-infra red (NIR) emissive compounds, processes of making the compounds thereof, and use of the compounds therein.

The present disclosure concerns the design, formulations, preparations and optical properties of compounds of Formulas (I) and (VI):

(charged dye.sup.m+).sub.x.Math.(counterion.sup.n).sub.y.Math.(counterion receptor).sub.z(I) and

(charged dye.sup.m).sub.x.Math.(counterion.sup.n+).sub.y.Math.(counterion receptor).sub.z(VI).

The charged dye.sup.m+ is a cationic dye, counterion.sup.n is an anion, and counterion receptor is a binding ligand for the counterion.sup.n, wherein m, n, x and y are integers greater than or equal to 1, and products of x.Math.n and m.Math.y are identical for formula (I).

The charged dye.sup.m is a anionic dye, counterion.sup.n+ is a cation, and counterion receptor is a binding ligand for the counterion.sup.n+, wherein m, n, x and y are integers greater than or equal to 1, and products of x.Math.n and m.Math.y are identical for formula (VI).

The present disclosure concerns the design, formulations, preparations and optical properties of compounds of Formulas (I) and (VI):

(charged dye.sup.m+).sub.x.Math.(counterion.sup.n).sub.y.Math.(counterion receptor).sub.z(I) and

(charged dye.sup.m).sub.x.Math.(counterion.sup.n+).sub.y.Math.(counterion receptor).sub.z(VI).

The charged dye.sup.m+ is a cationic dye, counterion.sup.n is an anion, and counterion receptor is a binding ligand for the counterion.sup.n, wherein m, n, x and y are integers greater than or equal to 1, and products of x.Math.n and m.Math.y are identical for formula (I).

The charged dye.sup.m is a anionic dye, counterion.sup.n+ is a cation, and counterion receptor is a binding ligand for the counterion.sup.n+, wherein m, n, x and y are integers greater than or equal to 1, and products of x.Math.n and m.Math.y are identical for formula (VI).

A fluorescent dye and quencher mixture for reporting on nucleic acid amplification from a sample includes a fluorescent intercalating dye, a dye sequestering or quenching agent such as hydroxynapthol blue (HNB) or caffeine, and primers, dNTPs, and a nucleic acid polymerizing enzyme or fragment thereof. The presence of the dye in combination with the dye sequestering or quenching agent improves the overall dynamic range of the fluorescent signal as well as shortens the time needed for visualization or image capture of amplified nucleic acid. The fluorescent dye and quencher mixture also enables the detection of nucleic acids in samples having low copy numbers.

A colored composition of the present invention includes a dye compound having an anionic moiety including at least one of a structure represented by the following General Formula (A1), a structure represented by the following General Formula (A2), and a structure containing a boron atom, and a dye structure having a cationic moiety, in which the anionic moiety and the cationic moiety are bonded to each other via a covalent bond and present in the same molecule; a curable compound; and a solvent.

##STR00001## (in General Formula (A1), R.sup.1 and R.sup.2 each independently represent SO.sub.2 or CO)

##STR00002## (in General Formula (A2), R.sup.3 represents SO.sub.2 or CO; and R.sup.4 and R.sup.5 each independently represent SO.sub.2, CO, or CN)

A colored composition of the present invention includes a dye compound having an anionic moiety including at least one of a structure represented by the following General Formula (A1), a structure represented by the following General Formula (A2), and a structure containing a boron atom, and a dye structure having a cationic moiety, in which the anionic moiety and the cationic moiety are bonded to each other via a covalent bond and present in the same molecule; a curable compound; and a solvent.

##STR00001## (in General Formula (A1), R.sup.1 and R.sup.2 each independently represent SO.sub.2 or CO)

##STR00002## (in General Formula (A2), R.sup.3 represents SO.sub.2 or CO; and R.sup.4 and R.sup.5 each independently represent SO.sub.2, CO, or CN)

This document provides materials and methods for a membrane transporter assay (e.g., an oscillating stimulus transporter assay (OSTA)). OSTA can be used to obtain temporal readouts of transporter activity and to screen for membrane transporter modulators (e.g., agonists or antagonists).

A positive-working lithographic printing plate precursor includes a coating optimized for producing a minimum extent of ablation when exposed to heat and/or light. The coating includes an infrared absorbing agent which contains a structural element according to Formula I:

##STR00001##

wherein A represents SR.sup.1 wherein R.sup.1 represents an optionally substituted alkyl, aralkyl, alkaryl, cycloalkyl, alkenyl, alkynyl, aryl, or heteroaryl group, and/or combinations thereof; and Q=CHRCHR, CRCR or CHRCHRCHR and R, R and R independently represent hydrogen, an alkyl, cycloalkyl, aralkyl, alkaryl, aryl or heteroaryl group, or R and R or R and R form together a cyclic structure.

Provided are a compound represented by any one of Formulae (1) to (3) described in the specification, a coloring composition for dyeing or textile printing including the compound, an ink for ink jet textile printing, a method of printing on fabric, and a dyed or printed fabric.