A method of preparing a functionalized electrode array is provided. The method includes depositing a conductive material onto the surface of a substrate by droplet-based printing of particles comprising an electrically-conductive material. The surface of the conductive material is functionalized with a binding reagent that binds to an analyte. A three-dimensional electrode array and microfluidic test device are also provided.

A method of preparing a functionalized electrode array is provided. The method includes depositing a conductive material onto the surface of a substrate by droplet-based printing of particles comprising an electrically-conductive material. The surface of the conductive material is functionalized with a binding reagent that binds to an analyte. A three-dimensional electrode array and microfluidic test device are also provided.

The present disclosure relates to a method of high yielding production of gas vesicle nanoparticles (GVNPs) and genetic tools used for high-yielding GVNPs production. The present disclosure further relates to a method of creating 3D tissue constructs with improved cell viability and proliferation and the resulting 3D tissue constructs. The GVNPs can promote cell growth and proliferation in 3D constructs and are suitable bioinks components for a bioprinter to build 3D structures through 3D printing as well as other applications.

The present disclosure relates to a method of high yielding production of gas vesicle nanoparticles (GVNPs) and genetic tools used for high-yielding GVNPs production. The present disclosure further relates to a method of creating 3D tissue constructs with improved cell viability and proliferation and the resulting 3D tissue constructs. The GVNPs can promote cell growth and proliferation in 3D constructs and are suitable bioinks components for a bioprinter to build 3D structures through 3D printing as well as other applications.

The invention provides a supramolecular polymer composition capable of co-assembly to maintain a three dimensional (3-D) macrostructural form after 3-D printing, made of a solvent, a template molecule; and a reactive component. The reactive component can be at least one monomer that is capable of hydrogen bonding with the template molecule to form a 1D supramolecular structure. The template may be an amphiphilic polymer. The monomer has at least two pendant groups capable of covalent crosslinking. The invention also includes a 3-D structure formed by crosslinking a 3-D printed supramolecular polymer composition, which optionally has a mesoporous structure. Also included is a method of manufacturing a 3-D structure by delivering a supramolecular polymer composition onto a surface of a substrate to form the 3-D structure.

A bio-ink comprising: (a) at least one polysaccharide; (b) at least one structural protein; and (c) fibrinogen, wherein the polysaccharide is selected from a group consisting of Sodium alginate Chitosan, Hyaluronic acid, Gellan gum, Dextran, Agarose, Poly(ethylene glycol), Pluronic and Carrageenan and the structural protein is selected from a group consisting of Gelatin, Collagen Vitronectin, Laminins and Fibronectin.

The present invention provides compositions, such as varnishes, ink vehicles, and finished inks, having high bio-renewable carbon (BRC) content. The compositions comprise protein and colophony.

The present invention provides compositions, such as varnishes, ink vehicles, and finished inks, having high bio-renewable carbon (BRC) content. The compositions comprise protein and colophony.

The present disclosure relates to a liver organoid, more specifically, to a liver organoid in which a liver lobule-type structure can be maintained for a long time and a preparation method using the same. The liver organoid of the present disclosure comprises: a tubular outer cavity the inside of which is divided into a plurality of compartments; a cell aggregate loaded into the compartments; and an inner cavity located at the core of the outer cavity.

Provided herein are methods for the preparation of perfusable scaffolds for cell culture. These methods can comprise providing a bioink composition and a fugitive ink composition; chaotic printing the bioink composition and the fugitive ink composition to generate a microstructured precursor comprising a plurality of lamellar structures formed from the bioink composition; curing the bioink composition to form a cured scaffold precursor; and removing the fugitive ink from the cured scaffold precursor, thereby forming the perfusable scaffold. Also provided are scaffolds prepared by these methods as well as modular bioreactors incorporating these scaffolds.