Provided is a composition that may include an aqueous phase, and extremophilic bacteria having signal transduction chemotaxis machinery and nanoemulsion oil droplets including metal-silica nanoparticles attached to a surface thereof. Further provided is a method for treating an oil reservoir, that may include introducing into the oil reservoir an extremophilic bacteria having signal transduction chemotaxis machinery and nanoemulsion oil droplets including metal-silica nanoparticles attached to a surface thereof. Further provided is method for bacteria mediated enhanced oil recovery that may include introducing extremophilic bacteria downhole to an oil reservoir. The method may further include triggering cell lysis of the extremophilic bacteria, mobilizing the oil trapped by the biosurfactants released from lysed extremophilic bacteria, and producing the oil trapped by the biosurfactants from the oil reservoir.

Provided is a composition that may include an aqueous phase, and extremophilic bacteria having signal transduction chemotaxis machinery and nanoemulsion oil droplets including metal-silica nanoparticles attached to a surface thereof. Further provided is a method for treating an oil reservoir, that may include introducing into the oil reservoir an extremophilic bacteria having signal transduction chemotaxis machinery and nanoemulsion oil droplets including metal-silica nanoparticles attached to a surface thereof. Further provided is method for bacteria mediated enhanced oil recovery that may include introducing extremophilic bacteria downhole to an oil reservoir. The method may further include triggering cell lysis of the extremophilic bacteria, mobilizing the oil trapped by the biosurfactants released from lysed extremophilic bacteria, and producing the oil trapped by the biosurfactants from the oil reservoir.

A method and a system for recovering oil from currently inaccessible oil containing geological units by activating the deep biosphere microbial seed bank. Nutrient and thermal enhancement of microorganisms in oil containing geological units allows for stimulation of inactive and/or dormant microorganisms such that they proliferate and produce gas. The oil viscosity that is decreased by heat, along with the gas pressure produced by activated microbes which allows previously inaccessible oil to flow toward production wells.

A method and a system for recovering oil from currently inaccessible oil containing geological units by activating the deep biosphere microbial seed bank. Nutrient and thermal enhancement of microorganisms in oil containing geological units allows for stimulation of inactive and/or dormant microorganisms such that they proliferate and produce gas. The oil viscosity that is decreased by heat, along with the gas pressure produced by activated microbes which allows previously inaccessible oil to flow toward production wells.

Provided is a composition that may include an aqueous phase, and extremophilic bacteria having signal transduction chemotaxis machinery and nanoemulsion oil droplets including metal-silica nanoparticles attached to a surface thereof. Further provided is a method for treating an oil reservoir, that may include introducing into the oil reservoir an extremophilic bacteria having signal transduction chemotaxis machinery and nanoemulsion oil droplets including metal-silica nanoparticles attached to a surface thereof. Further provided is method for bacteria mediated enhanced oil recovery that may include introducing extremophilic bacteria downhole to an oil reservoir. The method may further include triggering cell lysis of the extremophilic bacteria, mobilizing the oil trapped by the biosurfactants released from lysed extremophilic bacteria, and producing the oil trapped by the biosurfactants from the oil reservoir.

Provided is a composition that may include an aqueous phase, and extremophilic bacteria having signal transduction chemotaxis machinery and nanoemulsion oil droplets including metal-silica nanoparticles attached to a surface thereof. Further provided is a method for treating an oil reservoir, that may include introducing into the oil reservoir an extremophilic bacteria having signal transduction chemotaxis machinery and nanoemulsion oil droplets including metal-silica nanoparticles attached to a surface thereof. Further provided is method for bacteria mediated enhanced oil recovery that may include introducing extremophilic bacteria downhole to an oil reservoir. The method may further include triggering cell lysis of the extremophilic bacteria, mobilizing the oil trapped by the biosurfactants released from lysed extremophilic bacteria, and producing the oil trapped by the biosurfactants from the oil reservoir.

An iron-reducing Tessaracoccus oleiagri strain DH10 and applications of the iron-reducing Tessaracoccus oleiagri strain DH10 are provided. The Tessaracoccus oleiagri strain DH10 had been preserved in China center for type culture collection on Apr. 19, 2021, with a preservation number of CCTCC No: M 2021404. The iron-reducing Tessaracoccus oleiagri strain DH10 can efficiently reduce Fe(III) is separated and screened from oil reservoir environment. SEM analysis shows that it can effectively decompose lean iron montmorillonite minerals and inhibit expansion of clay. Moreover, core experiments show that biological agent of the Tessaracoccus oleiagri strain DH10 can reduce water sensitivity of reservoir core and water injection pressure, and can be applied to crude oil recovery to effectively improve crude oil recovery factor.

An iron-reducing Tessaracoccus oleiagri strain DH10 and applications of the iron-reducing Tessaracoccus oleiagri strain DH10 are provided. The Tessaracoccus oleiagri strain DH10 had been preserved in China center for type culture collection on Apr. 19, 2021, with a preservation number of CCTCC No: M 2021404. The iron-reducing Tessaracoccus oleiagri strain DH10 can efficiently reduce Fe(III) is separated and screened from oil reservoir environment. SEM analysis shows that it can effectively decompose lean iron montmorillonite minerals and inhibit expansion of clay. Moreover, core experiments show that biological agent of the Tessaracoccus oleiagri strain DH10 can reduce water sensitivity of reservoir core and water injection pressure, and can be applied to crude oil recovery to effectively improve crude oil recovery factor.

A hydrocarbon recovery method for recovering a production fluid containing hydrocarbons from a production well provided in the ground in which Type 1 microorganisms that produce a biological membrane (biofilm) and Type 2 microorganisms that produce carbon dioxide for promoting deposition of calcium carbonate exist, the hydrocarbon recovery method includes injecting, into the production well, a culture medium for increasing the Type 1 microorganisms; injecting, into the production well, a composition used for the Type 2 microorganisms to produce carbon dioxide, decompressing an inside of the production well after the culture medium and the composition are injected, and recovering the hydrocarbons in a state where the inside of the production well is decompressed.

A hydrocarbon recovery method for recovering a production fluid containing hydrocarbons from a production well provided in the ground in which Type 1 microorganisms that produce a biological membrane (biofilm) and Type 2 microorganisms that produce carbon dioxide for promoting deposition of calcium carbonate exist, the hydrocarbon recovery method includes injecting, into the production well, a culture medium for increasing the Type 1 microorganisms; injecting, into the production well, a composition used for the Type 2 microorganisms to produce carbon dioxide, decompressing an inside of the production well after the culture medium and the composition are injected, and recovering the hydrocarbons in a state where the inside of the production well is decompressed.