A method designed to clarify the coffee oil contained in coffee grounds or in whole and/or damaged coffee beans. The method objective is achieved by starting with inoculation of the coffee grounds or coffee beans with macromycetes especially with white rot fungi, continuing with an incubation, step that allows complete population of the coffee grounds or coffee beans by the fungal mycelium to be achieved, and finishing with steps of drying and extracting the coffee oil. The method disclosed allows colourless or pale yellow coffee oil to be produced, favouring the use thereof in cosmetic and food products, amongst others.

The invention provides novel and improved methods that allow effective capture of valuable active ingredients in biomass such as whole stillage or thin stillage at cost-effective commercial scale. The invention also provides novel compositions of active ingredients with unique properties (e.g., nutritional values and enhanced bioavailability).

The current invention relates to a method of separating polyunsaturated fatty acids containing lipids from a lipids containing biomass by using acetone.

In accordance with the invention, it was found, surprisingly, that a polyunsaturated fatty acids (PUFAs)-comprising biomass with other feedstuff components can be extruded at a low energy input of 12-28 Wh/kg to give an extrudate with a very high oil load capacity.

Methods for obtaining corn oil from milled corn germ (e.g., dry milled corn germ), involving adding water, at least one acidic cellulase, at least one acidic protease, and at least one phytase to milled corn germ to obtain corn oil.

Provided herein are microalgae of a Thraustochytrid and a method for preparing bio-oil using the same, and more particularly, Aurantiochytrium sp. LA3 (KCTC12685BP) having bio-oil producibility, and a method of preparing bio-oil, particularly bio-oil having a content of omega-3 unsaturated fatty acids of 30% by weight or more based on total fatty acids, characterized by culturing the microalgae. The microalgae Aurantiochytrium sp. LA3 (KCTC12685BP) described herein has a rapid sugar consumption rate when being cultured using glucose as a carbon source, has a high oil content, allows cells to be cultured at a high concentration, and allows oil to be obtained in high productivity and a high yield, and thus, may produce bio-oil more economically and environmentally friendly.

Apparatuses and methods for extracting desired oils, resins, and/or solids from biomass. An aspect of the present disclosure comprises a continuous flow system using solvents as a carrier liquid and then converting the solvent to a supercritical state for extraction of the desired materials through pressure, temperature, and volume control within the extraction system.

A process for production of biofuels from algae can include cultivating an oil-producing algae by promoting sequential photoautotrophic and heterotrophic growth. The method can further include producing oil by heterotrophic growth of algae wherein the heterotrophic algae growth is achieved by introducing a sugar feed to the oil-producing algae. An algal oil can be extracted from the oil-producing algae, and can be converted to form biodiesel.

Systems and methods are provided for separating high value by-products, such as oil and/or germ, from grains used for alcohol production. In one embodiment, a method for separating by-products from grains used for alcohol production includes, subjecting milled grains to liquefaction to provide a liquefied starch solution including fiber, protein, and germ. The germ is separated from the liquefied starch solution. The separated germ is ground, e.g., to a particle size less than 50 microns, to release oil to provide a germ/oil mixture. Then, prior to fermentation, the oil is separated from the germ/oil mixture to yield an oil by-product. The pH of the germ/oil mixture can be adjusted to about 8 to about 10.5 and/or cell wall breaking enzymes or chemicals may be added to help release oil from the germ. In one example, the oil yield is greater than 1.0 lb/Bu.

The present technology relates to processes of fermenting a starch-containing material into a fermentation product comprising a fermentation step in the presence of a xylanase in combination with a pectinase on oil partitioning during post-fermentation processing. In particular, the enzyme(s) were added during simultaneous saccharification and fermentation. The finished beer was subjected to beer well incubation, distillation, and then decanting to separate thin stillage from the solids.