In accordance with the invention, it was found, surprisingly, that a polyunsaturated fatty acids (PUFAs)-comprising biomass with other feedstuff components can be extruded at a low energy input of 12-28 Wh/kg to give an extrudate with a very high oil load capacity.

A method for oil recovery from biomass is provided which includes adding at least one lipase classified as EC 3.1.1.3 to a biomass prior to or during a fermenting of the biomass. The method can further include fermenting, recovering stillage and separating oil therefrom.

The present invention provides a solid fat composition that includes an oil having saturated fat and an oil having at least one long chain polyunsaturated fatty acid. In particular, the solid fat composition can have high levels of long chain polyunsaturated fatty acid and low to no presence of emulsifiers. In preferred embodiments, the polyunsaturated oil is an unwinterized microbial oil. The invention also relates to methods for making such compositions and food, nutritional, and pharmaceutical products comprising said compositions.

The present invention provides a method for extracting heat-sensitive Antarctic krill oil comprising the following steps. Firstly, frozen Antarctic krill is thawed and minced; a surfactant is added to the minced Antarctic krill to form a supercritical micelle system; extract the Antarctic krill multiple times after pressurizing and liquefying mixed gases; at the same time, a low-carbon alcohol solvent is added to strip the material in the critical micelle; after the extraction is completed, the extractant is removed; the extraction product and the residue after the extraction are subsequently collected; the heat-sensitive Antarctic krill oil can be obtained by high-speed centrifugal separation of the oil-water mixed extraction product. The present method has the advantages of simple operation, low extraction pressure and high safety. The extraction method of gas pressure liquefaction is completed under low temperature sealing conditions.

Disclosed herein are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by lysing the cells to form a lysed cell composition, treating the lysed cell composition to form an oil-containing emulsion and then recovering the oil from the oil-containing emulsion. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

The present invention relates to processes of recovering oil after liquefaction and/or from thin stillage and/or syrup/evaporated centrate from a fermentation product production process by adding a thermostable protease to the whole stillage, thin stillage and/or syrup.

SYSTEMS AND METHODS OF MAKING OIL FROM MICROORGANISMS Described herein are systems and methods of manufacturing an oil comprising a triacylglyceride. The systems and methods may comprise: (a) providing an oleaginous microorganism; (b) culturing the oleaginous microorganism in a medium comprising a carbon source, wherein the carbon source comprises ethanol at greater than 50% by weight; and (c) harvesting the oil from the oleaginous microorganism when the oil is at least 25% by weight of the oleaginous microorganism.

The current invention relates to a method of separating polyunsaturated fatty acids containing lipids from a lipids containing biomass.

Provided is a nutritionally enhanced derivative (isolate) from Stabilized Rice Bran (SRB) with improved antioxidant, fat and protein levels enhancing both the nutritional and yield values over existing techniques. Also provided is an improved method that utilizes certain enzyme combinations under various time and temperature conditions for extracting these nutritionally enhanced isolates from SRB.

The present invention contemplates the creation of a low fluoride crustacean oil processed from a phospholipid-protein complex (PPC) formed immediately upon a crustacean (i.e., for example, krill) catch. Further, the crustacean oil may also have reduced trimethyl amine and/or trimethyl amino oxide content. The process comprises disintegrating the crustaceans into smaller particles, adding water, heating the result, adding enzyme(s) to hydrolyze the disintegrated material, deactivating the enzyme(s), removing solids from the enzymatically processed material to reduce fluoride content of the material, separating and drying the PPC material. Then, using extraction with supercritical CO.sub.2 or supercritical dimethyl ether, and/or ethanol as solvents, krill oil, inter alia, is separated from the PPC. In the extraction the krill oil can be separated almost wholly from the feed material.