A method for the separate extraction of rice bran oil and rice bran wax using supercritical CO.sub.2 as extraction medium and including two separation steps involving different predetermined pressure and temperature conditions.

A method of applying energy to CBD oil is provided. The method may include obtaining CBD oil from a plant, combining the CBD oil with a carrier oil, heating the combination of the CBD oil and carrier oil, mixing the CBD oil and carrier oil, filtering the CBD oil and carrier oil to a size of less than 60 nanometers, and applying an energy field to the CBD oil and carrier oil through a laser effector such that an electron is promoted from a bonding or non-bonding orbital into an empty anti-bonding orbitals of the CBD molecule. The laser effector may be a pink sapphire cut into a 15 equal side faceted crystal, double-stacked in a 2.3 cm cylindrical chamber, or an opulence half dome bubble lens configured to refract the beam.

Methods and systems for the production and isolation of fatty acid methyl esters (FAMEs) from a lipid source are described. The method includes extracting a lipid from a lipid source and transesterifying the lipid into a FAME. The method may also include fractionating the FAME from the system. A method of selectively transesterifying a lipid into a FAME is also described.

A composition of matter comprises a shelf stable, partially defatted supercritical CO.sup.2 fluid solvent extracted whole grain flour derived from a cracked biomass of perilla frutescens, the flour comprising minerals and 2 to 8 percent of native seed oil, 35 to 45 percent protein, and 35 to 45 percent fiber.

The present invention contemplates the creation of a low fluoride crustacean oil processed from a phospholipid-protein complex (PPC) formed immediately upon a crustacean (i.e., for example, krill) catch. Further, the crustacean oil may also have reduced trimethyl amine and/or trimethyl amino oxide content. The process comprises disintegrating the crustaceans into smaller particles, adding water, heating the result, adding enzyme(s) to hydrolyze the disintegrated material, deactivating the enzyme(s), removing solids from the enzymatically processed material to reduce fluoride content of the material, separating and drying the PPC material. Then, using extraction with supercritical CO.sub.2 or supercritical dimethyl ether, and/or ethanol as solvents, krill oil, inter alia, is separated from the PPC. In the extraction the krill oil can be separated almost wholly from the feed material.

The invention provides systems and methods for the automation of the extraction of a liquid substance from a material. The disclosed systems include multiple tanks and valves under computer control providing for the safe transport of a solvent through the material under computer control. Solvent transport is accomplished by heating and/or cooling selected tanks or vessels to cause the solvent to evaporate or condense as desired. Tank and/or solvent heating and cooling is controlled automatically. Thus, in all embodiments the use of external pumps can be avoided.

A method for cell disruption and extraction of a plant, includes placing plant material on a porous container, placing the porous container into a reaction device, and producing water ion steam of an atmospheric pressure by a water ion generator. The water ion steam infiltrates the porous container and permeates the plant material. The water ion steam penetrate cells of the plant material and breaks the cell wall of the cells, to cause a cell disruption. The residual water ion steam is drained outward from the exhaust pipe of the box.

The disclosure provides a method of obtaining an isolated phospholipid enriched krill composition. The method includes a) contacting a crude krill composition with an alcohol in an amount sufficient to provide a phospholipid enriched layer and a non-phospholipid enriched layer; b) forming a phospholipid enriched layer and a non-phospholipid enriched layer, wherein the phospholipid enriched layer is above the non-phospholipid enriched layer; c) isolating the phospholipid enriched layer and the non-phospholipid enriched layer; and d) removing the alcohol from the phospholipid enriched layer to provide an isolated phospholipid enriched krill composition.

A system for extracting components from sample material includes a multi-port extraction vessel and an extraction basket that is configured to hold the sample material and to be rotated within the multi-port extraction vessel. The extraction vessel may include multiple ingress ports and multiple egress ports to allow a liquid solvent to flow through the extraction vessel during an extraction process. The extraction basket is permeable to allow liquid solvent to flow through the extraction basket and distribute throughout the sample material held within the extraction basket. A rotation mechanism, such as a motor, may rotate the extraction basket within the extraction vessel during the extraction process. The multi-port extraction vessel, in combination with the rotatable extraction basket, promotes even distribution of the liquid solvent throughout the sample material.

A method and an apparatus for providing coolants and/or extractants for at least one press, and to a pressing apparatus. The reliability and accuracy of the feed of a coolant and/or extractant to an extraction press that are needed for large-scale industrial use can be achieved by the combination of a storage device, a metering device and a distributing device.