Recombinant proteobacteria, including ?-proteobacteria, comprising a heterologous acyl-ACP desaturase and a heterologous acyl-ACP thioesterase, wherein the native dual 3-hydroxy acyl-ACP dehydratase/isomerase is deleted are provided herein. The recombinant proteobacteria produce non-native monounsaturated free fatty acids or derivatives thereof. Methods of producing non-native monounsaturated free fatty acids or derivatives thereof are also provided, in addition to cell cultures and fatty acid compositions produced by the recombinant proteobacteria. The recombinant proteobacteria may be used to produce insect pheromones or precursors thereof, and fragrances or precursors thereof.

The disclosed embodiments include systems and methods for the solvent extraction of a substance from a material. The disclosed systems include multiple tanks and an extraction chamber in communication with the tanks. Solvent transport is accomplished by heating and/or cooling selected tanks to cause the solvent to be transported between tanks.

Provided herein are systems and methods for the facile extraction and purification of oils from plant material, including cannabis and hemp. The systems and methods herein are versatile, and may utilize a wide range of solvents to extract oils from a variety of plant-based material. Further, the provided systems and methods are closed loop, reducing the loss solvent and decreasing the risk of safety concerns such as human exposure to solvent chemicals or explosion of volatiles. In some embodiments, the systems and methods remove impurities from the extracted oils, for example waxes or other precipitates, and provide a higher purity and higher quality extract.

A hollow vessel operated under vacuum receives whole orange peel delivered by a conveyor. The peel is heated indirectly by steam. Oil and water present in the peel evaporates and is drawn off to be condensed, captured and separated. Separation is by decantation. Operation under vacuum lowers the boiling point of the oil. Steam condensate is collected for reuse. The exiting peel discharges from the vessel and is conveyed away for further processing.

A system for managing learning programme outcomes, comprising: a database configured to maintain in non-transient memory: programme data records, stream data records, course data records, learning goal data records; assessment task data records, rubric data records, and trait data records; wherein the trait data record can be examination type or non-examination type, each examination type trait data record includes grade levels defined by a range of percentage examination mark result, and each non-examination type trait data record includes grade levels defined by a score of a quality level in a trait exhibited by a student; a programme and stream module for managing the programme data records and the stream data records; a course module for managing the course data records; a learning module for managing the learning goal data records; and an assessment module configured for managing the assessment task data records, rubric data records, and trait data records.

An extraction system comprises an extraction chamber having an end cap at an one end accepting organic matter and gas or liquid solvent. A collection vessel is attached below the extraction chamber, a viewing vessel is sealed at an acute angle to a sidewall of the collection vessel enabling visual inspection at the collection plate. A gas tank is connected to the extraction chamber introducing solvent and a frame supports chamber by axles fixedly mounted on opposite sides of the extraction chamber, said axles supported by the frame structure enabling free rotation of the chamber about the axles. After gas is introduced to the organic matter, the extraction chamber is rotated vigorously about the axis, the one end is then connected to the open upper end of the collection vessel, and a lipid yield is collected at the collection plate.

A multifunctional continuous phase transition extraction apparatus comprises an extraction system, a desorption system and a solvent recovery system. The extraction system comprises a first heat exchanger and an extraction tank. The desorption system comprises a second heat exchanger, a first desorption tank, a second desorption tank, a first purification column and a second purification column. The solvent recovery system comprises a first condenser, a second condenser, a first solvent tank and a second solvent tank. Two extraction loops can be formed in the present invention. During the overall extraction process, the phase transition process of the extracting agent is real-time and continuous. The extracting agent goes through continuous phase-transitions and is cyclically re-used. The overall process is operated in an airtight and low-pressure condition, and is multifunctional, safe, reliable, and suitable for the extraction for most natural products.

Methods for the extraction of intracellular contents from biomass, in particular from algae, are described. The pH level of the algae cell culture is modified and mixed with a surfactant or a combination of surfactants. The algal culture may be subjected to low pressurization using air, inert gas or a gas mixture. Separation of the lipid contents from the treated algae culture is accomplished with an organic solvent or a mixture of organic solvents. The algal debris may be collected at the bottom of the aqueous phase while the biomass is concentrated in the solvent layer.

The invention relates to a method for aggregating and separating an organic material mixture which is provided in a dissolved form in an aqueous emulsion. The method is characterized by the following steps: a) providing an aqueous emulsion with organic compounds which are provided in the emulsion in a dissolved form, said organic compounds being carboxylic acids, phospholipids, glycolipids, glyceroglycolipids, phenols, sterols, chlorophyll, and/or sinapines, b) mixing the emulsion from step a) with an aqueous solution containing copper(II) ions and/or calcium ions until an aggregate formation is achieved, and c) separating the aggregates from step b) by means of a sedimentation, filtration, or centrifugation process after achieving an aggregated phase of the organic compounds from step b).

Compositions for increasing corn oil recovery and embodiments of methods for using the composition for corn oil separation are described. The composition(s) incorporate an admixture that includes a polymer selected from a polyglycol ester, a polyethyleneoxide-polypropyleneoxide block copolymer, a poloxamine, or a mixture thereof. The methods include admixing the compositions with a process stream for, for example, the extraction of oil from milled corn and residues from a fermentation step, including stillage (e.g., thin stillage or mid stillage), distiller's wet grain, distiller's dry grain and distiller's dry grains with solubles.