Disclosed herein are compositions and methods of fixing and staining rare cells. Further, disclosed herein are methods of identifying circulating tumor cells (CTC). In some embodiments, the method includes: imaging a cell sample to identify a cell of interest; determining a first pixel intensity of a stained nuclear area; determining a second pixel intensity of a background area; calculating a ploidy status of the cell of interest by subtracting the second pixel intensity from the first pixel intensity; and determining whether the cell of interest is a CTC based on the ploidy status. The method may be computer implemented, such that the method uses a machine learning algorithm to identify a feature; process the feature to extract a parameter of interest; analyze the parameter of interest; and when the parameter of interest is greater than or less than a pre-determined threshold, classify the cell of interest as a CTC.

This invention relates to compositions and methods for cleaning, disinfection, and sterilization of hard, soft, and porous surfaces, equipment, human skin, tissues, food and vegetable surfaces, and other media which are contaminated with microorganisms such as bacteria, viruses, yeast, and molds. Particularly relevant is the microbial environment whereby bacterial communities can generate three-dimensional polymicrobial Extracellular Polymeric Substances, or EPS, biofilm communities supported by an aggregation of microorganisms growing on a substrate layer, substantially degrading the antimicrobial performance of typical cleaners, disinfectants, and sterilizers. There is great need for a composition that will reliably eradicate microorganisms across a broad spectrum, particularly polymicrobial EPS communities shrouded by self-generated biofilm layer. Such a composition is disclosed herein. The invention discloses new compositions, and methods for formulation of compositions, of weak acids, surfactants, and glycol monoesters, which achieve a level of eradication such that when tested following application, no surviving microorganisms remain. All compositions and methods disclosed herein have the further benefit of their ingredients being classified by the US Food and Drug Administration (FDA) as GRAS, or Generally Regarded As Safe for use on food and food contact surfaces; and the embodiment of the claimed invention herein contains 95% USDA Certified Biobased content, and is accepted to the USDA BioPreferred® Program.

A unit dose detergent pack includes a pouch made of a water-soluble film and a detergent composition encapsulated within the pouch. The detergent composition consists essentially of a rheology modified surfactant system and at least one adjunct. The rheology modified surfactant system consists of a surfactant blend including an alcohol ethoxy sulfate having a C.sub.8-C.sub.20 backbone that is ethoxylated with from about 1 to about 10 moles of ethylene oxide and is present in an amount of from about 5 to about 30 weight percent actives. The detergent composition also consists essentially of a rheology modifying agents consisting of water present in a total amount of from about 5 to about 30 weight percent and a glycol ether present in an amount of from about 0.5 to about 20 weight percent.

A package comprising at least one layer of biodegradable material and containing a plurality of unit dose products, at least one unit dose product comprising a detergent composition comprising hydrogenated castor oil within a sealed compartment formed by a water soluble film.

Laundry detergent formulations for everyday use having cleaning and enhanced germ removal efficacy, as well as consumer acceptable viscosity levels and long-term stability profiles, are disclosed.

Water-soluble refill dose articles have a water-soluble film defining a first compartment enclosing a concentrated cleanser composition. The concentrated liquid cleanser composition comprises an anionic surfactant, 0.1% by weight to 10% by weight water, and 20% or less by weight glycol. The anionic surfactant contributes to the % by weight water, and adding the article to water at a 1:1 to 1:20 ratio defines a cleanser volume and produces a liquid cleanser that is dispensable through a pump that forms foamed cleanser and has a viscosity within a range of 1 cps to 1000 cps. Kits having a dispenser and the water soluble refill dose article are disclosed that have a pump that can foam the liquid cleanser.

Single compartment water-soluble refill dose articles having a water-soluble film enclosing a liquid concentrated cleanser composition, methods of making the same, and kits are disclosed. The liquid concentrated cleanser composition includes a mixture of a rheology modifier, a first surfactant, a stabilizing amount of a first alkaline substance, a viscosity increasing amount of an additional alkaline substance that interacts with the rheology modifier, when diluted with water to form a cleanser, to increase the viscosity, and about 10% or less wt/wt water and about 20% or less by wt/wt glycol. The first alkaline substance is an ethoxylated amine and prevents the viscosity increasing amount of additional alkaline substance from reacting with the rheology modifier before water dissolves the water-soluble film. When dissolved in water at a 1:1 to 1:20 ratio, a cleanser has a viscosity within a range of 2000 cps to 50,000 cps is formed.

Water-soluble refill dose articles for forming a cleanser have a water-soluble film defining a first compartment enclosing a first composition and a second compartment enclosing a second composition, which are individual separate articles or are part of an integral article. The first composition has a rheology modifier, a first surfactant, 10% or less by weight water, 20% or less by weight glycol, and the first surfactant does not activate the rheology modifier. The second compartment has an alkaline substance that when mixed with the first composition and water activates the rheology modifier to increase the viscosity of a resulting cleanser and 10% or less by weight water. When the article is diluted in water at a 1:1 to 1:20 ratio, it produces a cleanser having a viscosity within a range of 2000 cps to 50,000 cps.

The present disclosure relates to cleaning compositions that are used to clean semiconductor substrates. These cleaning compositions can remove the defects/contaminants arising from previous processing on the semiconductor substrates and thereby make the substrates appropriate for further processing. The cleaning compositions described herein primarily contain at least one pH adjusting agent and at least one biosurfactant.

The invention relates to the use of a rhamnolipid in a surfactant system for handwash detergents to confer to the consumer a sensorial impression of mildness on the hands.