A device (30) includes a base connector (32) having an opening (33) and an impeller connector (64) coupled to the base connector (32). The impeller connector (64) has a through-passage (66) aligned with the opening (33) of the base connector (32). Further, the device (30) includes a flexible tube (34) having a first end (36) and a second end (40), where the first end (36) of the flexible tube (34) is coupled to the impeller connector (64). Furthermore, the device (30) includes a seal component (38) and an impeller (42) coupled to the second end (40) of the flexible tube (34). Additionally, the device (34) includes an enclosure (46) disposed enclosing the impeller (42), the flexible tube (34), the impeller connector (64), and the base connector (32).

A multi-chamber single-use bioreactor for cell culture expansion has bag assembly and a rigid support structure defining a bag receiving space. The bag assembly disposed in the bag receiving space of the rigid support structure and supported by the rigid support structure. The bag assembly has at least a first flexible bag and a second flexible bag. The first bag defines a first reaction chamber, and the second bag defines a second reaction chamber. The first reaction chamber has a first volume, a first inlet, and a first outlet, and the second reaction chamber has a second volume different from the first volume, a second inlet, and a second outlet. The second inlet of the second bag is fluidically connected to the first outlet of the first bag so liquid in first reaction chamber can be transferred to the second reaction chamber.

The present invention relates to a process for the production of in vitro engineered tissues, also known in the art as cultured meat, cultivated meat, cell based meat, cellular meat and/or clean meat, and a device for the production of the same. The process comprises the steps of: loading sterilizable 3D scaffolds into a seeding chamber, sterilization of the scaffolds in the seeding chamber, seeding the scaffolds by loading a first volume of culture medium into the seeding chamber, wherein the first volume of culture medium has a density of cells in suspension of 5,000 to 25,000 cell/cm.sup.2, more preferably from 10,000 to 16,000 cm.sup.2 such that the scaffolds are immersed in the culture medium, leaving the scaffolds and the first volume of culture medium in the seeding chamber for a period of 2-24 hours at 18-37° C., loading a second volume of culture medium into a bioreactor, the second volume being greater than the first volume of the culture medium wherein an incubation position of one or more movable grids inside the bioreactor confines the scaffolds to the second volume of the culture medium such that they remain immersed during an incubation step, wherein the scaffolds and the second volume of culture medium are incubated in the bioreactor for a period of 10-60 days at 18-37° C. and at a pH between 6.5-8.0, more preferably 7.0-7.4. The invention also relates to a device for the production of cultured meat.

A system includes a bioprocessing vessel formed of a material insulative to a liquid medium when present therein. At least one sensor is for sensing a parameter of the bioprocessing vessel and generating a signal indicative of the parameter. At least ne conductor is adapted to provide electrical communication between the liquid medium in the bioprocessing vessel and an external structure to achieve equipotential for reducing noise in the signal produced by the at least one sensor. Related apparatus and methods are also disclosed.

Provided herein are systems and methods for thermally separating a flask and the base of an automatic flask stirring device. A thermal separator apparatus may be provided with a stand and a plurality of first standoffs. The stand may have a top surface configured to support a flask containing a substance to be stirred by a flask stirrer. The first standoffs may be located between the stand and a flask stirrer base, thereby creating a first air gap between the stand and the flask stirrer base. In some embodiments, the first air gap allows air to flow straight through the first air gap in a first direction.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

An impeller, for example, a Rushton impeller for a bioreactor system is disclosed. The impeller includes a hub, optionaly including a slot, a plurality of blades, and one or more turbulators. The plurality of blades is disposed along a circumferential direction of the hub and spaced apart from each other. Each of the plurality of blades is coupled to at least a portion of a circumference and/or a top surface of the hub. Each blade of the plurality of blades includes a pressure face and a suction face. The one or more turbulators is disposed on at least a portion of the suction face, the pressure face, or both, of a blade of the plurality of blades.

A bioreactor cleaning system for cleaning a bioreactor in a rail vehicle. A suction unit, a supply unit, an electronic control unit for actuating the suction unit and the supply unit, an acid tank, a collection tank for receiving a liquid suctioned out of the bioreactor, and a freshwater connection are provided. The bioreactor cleaning system comprises a metering unit which has acid canister connections, base canister connections, an acid metering device which can be connected to the acid canister connections and the acid tank, and a base metering device which can be connected to the base canister connections and the acid tank. Additionally, a mixer is provided in order to mix the different liquids.

There is disclosed a modular anaerobic digestion point-of-waste to renewable energy system. The system is directed to a modular and scalable anaerobic digestion system for point-of-waste use. The System includes a pretreatment process for removing inhibitory nutrients from a feedstock, an in-treatment process for providing clean renewable energy and a post-treatment process for further providing clean renewable energy for subsequent use. The System includes a leaching bed; a liquids tank; a mixing tank; an anaerobic digester reactor; a precipitation tank; a stripping tank; a hydrogen sulfide scrubber; a water remover; a gas bladder; a dewaterer; and a flare system.

A method for filtering a gas includes passing a gas into a compartment of a casing that includes a polymeric film, the casing having: a first sleeve having a first outlet opening, a first filter being at least partially disposed within the first sleeve so that gas passing through the first sleeve must pass through the first filter; and a second sleeve having an second outlet opening, a second filter being at least partially disposed within the second sleeve so that gas passing through the second sleeve must pass through the second filter, the second sleeve being closed so that the gas passing into the compartment of the casing passes through the first sleeve and the first filter and directly contacts at least a portion of the first sleeve comprised of the polymeric film but does not pass through the second sleeve. Opening the second sleeve when a predetermined condition is met so that the gas passes through the second sleeve and the second filter.