A microfluidic dual-well device is disclosed. The device comprises: (a) a first substrate having a first end, a second end, and a culture microwell forming portion; (b) a plurality of culture microwells; (c) a second substrate having a first end, a second end, and a capture microwell forming portion, the two ends of the second substrate being respectively bounded to the two ends of the first substrate; (d) a plurality of capture microwells; (e) a microfluidic channel; (f) a microfluidic inlet port; and (g) a microfluidic outlet port; wherein the microfluidic channel is in fluidic connections with the culture microwells, the capture microwells, and the inlet and outlet ports. Methods of capturing and transferring a single cell or a single cell colony for culture, and method of transferring a target cell from a polydimethylsiloxane (PDMS) structure of culture microwells to a culture plate for culture are also disclosed.

A cell culture device configured for activating, transducing, isolating and/or expanding cells. The cell culture device includes a mixing tank and a rotary control platform. The mixing tank includes a first support, a hollow tank and a cover. The hollow tank is disposed on the first support and has a first opening, a second opening and an accommodating space. The cover covers the second opening and has a first hole, an inlet end and an outlet end. The rotary control platform includes a base, a second support, a rotating gear, an inclination control member and a micro injector. The second support is disposed on the base. The rotating gear is fixed on the first support. The inclination control member has a first part, a second part and a third part. The first part is connected to the rotating gear, and the second part is connected to the first part and the third part. The micro injector extends to the accommodating space through the first hole of the cover. The micro injector has a top end and a bottom end, the top end is fixed on the third part, and the bottom end is disposed to a bottom of the accommodating space. The micro injector is inclined towards the bottom of the accommodating space.