A well plate comprises a plate main body and at least one cavity in an upper side of the plate main body. An upwardly open annular channel is formed in the at least one cavity, the annular channel being delimited at an inner circumference thereof by a closed circumferential wall. A horizontal outer circumference of the circumferential wall decreases from bottom to top up to an upper edge of the circumferential wall. Within the horizontal circumference of the circumferential wall, at its upper edge, at least two retaining elements connect upwardly to the upper edge of the circumferential wall. The at least two retaining elements are at a free horizontal distance to one another, and at least one of the at least two retaining elements is elastically supported at the plate main body in horizontal direction.

A method displacing a fluid and simultaneously gas enriching a liquid cell culture medium with a gas. The method includes injecting a controlled volume of a gas or gas mixture into a one chamber by using a gas flow controller, the injection taking place through a gas inlet into a volume of liquid. This injection produces bubbling and agitation of the volume of liquid; a build-up of gas or gas mixture due to buoyancy in a hermetic space formed by the volume of liquid and the chamber, and a pressure increase in the chamber until a sufficient controlled pressure is reached of less than or equal to 10 bar. This increase displaces the volume of liquid by a fluid outlet connecting the volume of liquid to the exterior of the chamber. Also provided are a device implementing the method and a cell culture system in a multi-well culture plate.

A method of controlling a needle actuator to interact with a cell is provided, the method comprising: providing an actuator comprising a tower, a stage and a needle, wherein the needle is mounted on the stage; applying an electrostatic potential between the tower and the stage to retract the needle; moving the actuator towards the cell; reducing the potential so as to allow the stage and needle to move towards the cell; applying calibration data to detect when the needle has pierced the cell; and reducing the potential further once it has been detected that the needle has pierced the cell. The cell can be a biological cell. The needle can be a micro-needle and the stage can be a micro-stage.

The invention provides a resin molding and a method of producing the same in which sterilizability thereof is secured and discoloration (yellowing) thereof is reduced even when the resin molding is radiation-irradiated under anaerobic conditions (deoxidation conditions). The radiation-irradiated and packaged resin molding is primarily packaged with a packaging material having oxygen permeability and then secondarily packaged with an oxygen impermeable packaging material together with a deoxidizer. The method of producing a radiation-irradiated and packaged resin molding involves a step of primarily packaging a resin molding before radiation irradiation with a packaging material having oxygen permeability, a step of subsequently secondarily packaging with an oxygen impermeable packaging material together with a deoxidizer, and then a step of conducting radiation irradiation. A step of opening the secondary packaging under the atmosphere, following the radiation irradiation step, is preferred.

Provided is a cell culture vessel for culturing cells with a high density having excellent gas permeability and strength. A bag-shaped cell culture vessel of a closed system includes at least one port and mutually opposed planar substrates. At least one of the planar substrates is formed of a gas permeable film, the at least one gas permeable film has an outer surface provided with a protruding portion having a shape different from an inner surface shape of the gas permeable film, a culture space for culturing cells is provided in the inner surface side of the gas permeable film, and when the gas permeable film is brought in contact with a planar surface, a space enabling ventilation is formed between the gas permeable film and the planar surface by the protruding portion.

The present invention relates to a device and a method for the analysis of cultured cells, comprising a plurality of culture vessels, optical sensors and a detection system with detectors for determining a physico-chemical parameter in a culture vessel and for imaging the morphology of cells in a vessel. The device comprises a microscope adapted to be moved and having an optical path provided with switching means to switch between an optical path for determining a physico-chemical parameter and an optical path for imaging the morphology of cells.

A lab-on-chip type system, capable of identifying antibiotic sensitivity at the point of care of patients, especially in rural areas, clinics, hospitals that do not have care 24/7, hospitals with low level of equipment, among others, from the extraction of a sample, comprising a micro device or medical base device and a dispenser, wherein said micro device comprises a plurality of microwells, arranged in a circular fashion on one of the faces of the micro device, and wherein the micro-dispenser comprises a central plunger for taking and supplying the sample, a chamber for storage and distribution of the sample and a plurality of microdispensers arranged in a circular manner through which the sample is introduced into the microwells of the micro device.

A lab-on-chip type system, capable of identifying antibiotic sensitivity at the point of care of patients, especially in rural areas, clinics, hospitals that do not have care 24/7, hospitals with low level of equipment, among others, from the extraction of a sample, comprising a micro device or medical base device and a dispenser, wherein said micro device comprises a plurality of microwells, arranged in a circular fashion on one of the faces of the micro device, and wherein the micro-dispenser comprises a central plunger for taking and supplying the sample, a chamber for storage and distribution of the sample and a plurality of microdispensers arranged in a circular manner through which the sample is introduced into the microwells of the micro device.

A system and method for printing cells in a medium. A multi-dimensional printer, stably constructed of low-mass parts, can include a computer numerically controlled system that can enable motors driving delivery systems. The motors can include encoders that can enable achieving arbitrary resolution. The motors can drive ballscrews to enable linear motion of delivery systems, and the delivery systems can enable printing of a biological material in a pre-selected pattern in a petri dish. The petri dish can accommodate a medium such as a gel, and can further accommodate a vision system that can detect actual position and deflection of the delivery system needle. The printer can accommodate multiple delivery systems and therefore multiple needles of various sizes.

A Cell Migration Assay Plates (CMAP) assembly for high throughput microfluidic migration assays and method of manufacturing thereof are provided. The CMAP assembly includes a top plate having a plurality of wells aligned with a trough component having a plurality of troughs. Each of the wells is defined at least in part by first and second reservoirs and a divisional wall extending between the reservoirs. The trough component is secured to the top plate to form a plurality of micro-channels, such that each one of the micro-channels is defined by a portion of one of the divisional walls and a portion of a corresponding one of the plurality of troughs. The micro-channels enable communication between the reservoirs and visualization of cells migrating through the micro-channels. In this manner, migration of cells through the micro-channels can be visualized for testing and screening applications. A sealing component includes a trough gasket which is operable to be positioned against the bottom end of the well such that the sealing component is sandwiched between the divisional wall and the trough component. The trough gasket is operable to retain the plurality of cells within the troughs such that the plurality of cells migrating towards the second reservoir are isolated within the corresponding trough. At least a portion of the trough component is reconfigurable in relation to the trough gasket and the top plate such that the troughs are exposed to permit a user to retrieve one or more of the cells from the troughs.