A method of testing an antibiotic susceptibility includes dispensing and cultivating sample solution into culture wells including one or more comparative wells and a plurality of antibiotic wells receiving two or more kinds of antibiotics, respectively, receiving the sample solution into a plurality of preprocessing wells each including magnetic particles and fluorescent particles that bond to one or more kinds of bacteria such that the bacteria and the magnetic particles and fluorescent particles bond to each other, receiving the sample solution into a plurality of image wells having magnetic members thereunder such that the magnetic particles bonding to the bacteria are arranged on the bottoms of the image wells, removing the sample solution from the image wells that have undergone the planarizing step, taking fluorescent images of the image wells washed in the washing step, and determining an antibiotic tolerance/susceptibility of the sample solution by analyzing the fluorescent images.

The present invention provides a cell culture apparatus. A cell culture apparatus according to one embodiment of the present invention is a cell culture apparatus for culturing a cell to form the cell into a three-dimensional spheroid. The cell culture apparatus may comprise: a plate; and a well which is arranged, in plural, on one side of the plate at predetermined intervals, has cells cultured inside, and has a micropattern disposed on the inner surface such that the inner surface and the cells are spaced apart from each other.

The present invention provides a cell culture apparatus. A cell culture apparatus according to one embodiment of the present invention is a cell culture apparatus for culturing a cell to form the cell into a three-dimensional spheroid. The cell culture apparatus may comprise: a plate; and a well which is arranged, in plural, on one side of the plate at predetermined intervals, has cells cultured inside, and has a micropattern disposed on the inner surface such that the inner surface and the cells are spaced apart from each other.

The present disclosure relates to apparatuses, systems and methods for culturing cells. In particular, devices and methods are provided for generation and culture of 3d cell aggregates.

The disclosure relates to the fabrication of a three dimensional [3-D] cell culture membrane comprising one or more functionalized surfaces adapted to provide cell culture conditions suitable for the analysis of proliferation, differentiation or function of cells, typically eukaryotic or prokaryotic cells.

A planar patch clamp device is disclosed, which can be used for culturing a neuron in the device so as to form a neuron network, and detecting an electrical property of the neuron that forms the neuron network. The planar patch clamp device includes a plurality of protrusions formed on a first surface, an extracellular matrix forming substance which is coated on the peripheries of a through hole, and electrode sections.

A method for displacing a fluid and simultaneously gas enriching a liquid cell culture medium with a gas. The method includes injecting a controlled volume of a gas or gas mixture into a one chamber by using a gas flow controller, the injection taking place through a gas inlet into a volume of liquid. This injection produces bubbling and agitation of the volume of liquid; a build-up of gas or gas mixture due to buoyancy in a hermetic space formed by the volume of liquid and the chamber, and a pressure increase in the chamber until a sufficient controlled pressure is reached of less than or equal to 10 bar. This increase displaces the volume of liquid by a fluid outlet connecting the volume of liquid to the exterior of the chamber. Also provided are a device implementing the method and a cell culture system in a multi-well culture plate.

Described herein is a cell culture system for constructing a perfusable network of self-assembled cells comprising a multi-well plate embedded with microchannels connecting a central well with at least one inlet well and at least one outlet well, the central well for culturing seeded cells within an extracellular matrix, wherein the perfusable network allows perfusion through the microchannels connecting the central well with at least one inlet well and at least one outlet well. The cell culture system allows the array of perfusable networks formed, connected, and perfused inside the multi-well plate to be accessible and/or extractable from the top of the central well. In aspect, the cell culture system can improve the experimental throughputs of organ-on-a-chip systems and expand the application of microphysiological systems to regenerative cell therapy. A perfusable network of self-assembled cells and method of making thereof using the cell culture system described herein are also provided.

A cell culture device is provided. The cell culture device according to an exemplary embodiment of the present invention comprises: a body which includes a plurality of culture spaces formed therethrough in the height direction; and a plate-shaped cover member which is fixed to the bottom surface of the body while covering the open bottom portions of the culture spaces to thus form a culture surface where cells are cultured, and is surface-modified so as to allow the cells to be effectively attached to the culture surface.

The invention is a device for quantitatively collecting pooled samples from multiwell plates.