An apparatus for collecting or culturing cells or cell colonies includes: a common substrate formed from a flexible resilient polymeric material and having a plurality of wells formed therein; and a plurality of rigid cell carriers releasably connected to said common substrate, with said carriers arranged in the form of an array, and with each of the carriers resiliently received in one of the wells. A method of collecting or culturing cells or cell colonies with such an apparatus is carried out by depositing a liquid media carrying cells on the apparatus so that said cells settle on or adhere to said the carriers; and then (c) releasing at least one selected carrier having said cells thereon by gradual application of release energy to each carrier from the cavity in which it is received (e.g., by pushing with a probe).

The invention relates to a biocompatible scaffold for three dimensional cultivation of cells, said scaffold comprise one or more fibers randomly oriented to form a scaffold with open spaces for cultured cells. The one or more fibers are also coated with a bio-active coating and have a diameter of 100-3000 nm.

The present invention is drawn to the generation of micropatterns of biomolecules and cells on standard laboratory materials through selective ablation of a physisorbed biomolecule with oxygen plasma. In certain embodiments, oxygen plasma is able to ablate selectively physisorbed layers of biomolecules (e.g., type-I collagen, fibronectin, laminin, and Matrigel) along complex non-linear paths which are difficult or impossible to pattern using alternative methods. In addition, certain embodiments of the present invention relate to the micropatterning of multiple cell types on curved surfaces, multiwell plates, and flat bottom flasks. The invention also features kits for use with the subject methods.

Primary mammalian cell culture systems, methods of making primary mammalian cell culture systems, and methods of culturing primary mammalian cells are provided. In some embodiments the systems and methods utilize an exothermic chemical process as a heat source for culturing and shipping cultures comprising primary mammalian cells.

An improved tissue engineering bioreactor and testing platform has been designed that integrates multiple testing and stimulation capabilities. The system allows for growth of multiple tissue strips in parallel with mechanical and electrical stimulation, media perfusion, and the automated monitoring of contractile force and extracellular electrical activity. The system is designed to be low-cost and scalable, to provide for high-content, biofidelic, non-destructive testing of engineered muscle tissue performance that is conventionally measured using muscle-bath systems.

A embryo culture dish comprising: a first sidewall; a second sidewall attached to the first sidewall; a third sidewall attached to the second sidewall; a fourth sidewall attached to the third and first sidewalls, the four sidewalls forming an outer perimeter that forms a generally rectangular shape; a dish floor that is generally in communication with the four sidewalls, the dish floor comprising: a top surface that is at a first elevation; at least one rectangular shaped embryo culture well, the at least one rectangular shaped embryo culture well comprising: an outer perimeter that has generally a rectangular shape; a first slanted wall that comprises a first side of the rectangular shaped embryo culture well; a second slanted wall that comprises an opposite side from the first side of the rectangular shaped embryo culture well, the first and second slanted walls extend downward and towards each other until they both intersect with a well floor of the rectangular shaped embryo culture well, the well floor having a well top surface, and where the well top surface is at a second elevation, the second elevation being below the first elevation; at least one shaped well, the shaped well comprising: an outer perimeter that has generally a shape; a shaped well floor, the shaped well floor having a shaped well floor top surface, and where the shaped well floor top surface is at a third elevation, the third elevation being below the first elevation. A method of using a square time-lapse dish, the method comprising: adding a medium to a square time lapse dish; tilting the square time-lapse dish to an angle α; removing excess medium from a large well in the time-lapse dish, with the square time-lapse dish at angle α; and returning the square time-lapse dish to a horizontal orientation.

A system for isolating a target molecule from a bioprocess fluid includes a single-use disposable separation device having a plurality of perimeter-bonded layers defining one or more mesofluidic channels of the separation device, wherein each layer includes a biocompatible polymer material, wherein the separation device is configured to separate at least a portion of particles from the bioprocess fluid to generate a substantially clarified bioprocess fluid, and a chromatography system fluidically coupled at the outflow of the separation device in a configuration for further processing the clarified bioprocess fluid.

Provided is a large-scale cell culture system for producing products without harming animals. Also provided is a method for making meat products using this cell culture system. Further provided is a method for making personal care products using this cell culture system, as well as a method for making nutritional supplements using this cell culture system.

The invention relates to a multi-well plate comprising a support, the upper surface of which is at least partially covered with a continuous layer of a hydrogel in contact with the lower surface of a bottomless multi-well plate, the support, the continuous layer, and the bottomless multi-well plate being adhered by means of an adhesive which extends from at least certain portions of the lower surface of the bottomless multi-well plate up to certain portions of the upper surface of the support by passing through the continuous layer of hydrogel, each well of the bottomless multi-well plate being entirely surrounded by the at least certain portions of the lower surface. The application also relates to a method for preparing the multi-well plate and the use thereof for in vitro cell culture.

A sample testing apparatus includes a sample tray defining a planar surface and a plurality of wells recessed relative to the planar surface, and a lid member configured to be sealed about the planar surface of the sample tray. The lid member includes an adhesive layer configured to be sealed to the planar surface of the sample tray, a breathable film layer disposed about the adhesive layer, and a backing layer disposed about the breathable film layer. Methods of using the sample testing apparatus for testing a sample and kits to facilitate such testing are also provided.