The cell evaluation device includes a cell housing unit 111, and an adapter 120 configured to be elastically deformed by being fitted into the cell housing unit to close a top opening of the cell housing unit, and the adapter includes a body 121 to be inserted into the cell housing unit, a positioning unit 122 configured to position the body in a vertical direction with respect to the cell housing unit, a culture medium inlet port 124 and a culture medium outlet port 125 penetrating the top end and the lower end of the body, and a flexible sheet member 123 which is provided on a lower end of the body and extends radially outward from an outer edge of the lower end. This makes it possible to prevent leakage of the culture medium in the cell evaluation device.

An apparatus for measuring an electrophysiological signal of a three-dimensional (3D) cell and a method of fabricating the apparatus. The apparatus includes a flexible substrate including a plurality of protrusions, and a conductor formed on the plurality of protrusions, and regions other than the plurality of protrusions are partially removed from the flexible substrate.

A disposable polymer container for manipulation of small specimens adapted to optimize heat transfer between an external heating element and specimens herein contained. In particular, this invention relates to the field of containers for Assisted Reproductive Technology hereunder In-vitro fertilization (IVF).

A lab-on-a-chip (LOC) for the biomimetic study of the multicellular interactions of bone cells includes a PDMS substrate and cap, which together form one or more wells that are fluidly coupled by tubes. The wells are configured to support various bone cells and related cellular support substrates therein, while the tubes allow conditioned medium (CM), including soluble signals, and various other co-factors to be communicated among the various wells. By controlling the configuration among and between various bone cells in the wells, the temporal and spatial limitations associated with traditional in vivo bone tissue models is removed. In addition, the LOC enables a particular research objective to be studied by allowing the user to configure the arrangement of the wells/tubes of the LOC, so as to control the manner in which bone cell soluble signals, bone cell contact, and bone cell matrix interaction interplay.

An apparatus for collecting or culturing cells or cell colonies includes: a common substrate formed from a flexible resilient polymeric material and having a plurality of wells formed therein; and a plurality of rigid cell carriers releasably connected to said common substrate, with said carriers arranged in the form of an array, and with each of the carriers resiliently received in one of the wells. A method of collecting or culturing cells or cell colonies with such an apparatus is carried out by depositing a liquid media carrying cells on the apparatus so that said cells settle on or adhere to said the carriers; and then (c) releasing at least one selected carrier having said cells thereon by gradual application of release energy to each carrier from the cavity in which it is received (e.g., by pushing with a probe).

A culture medium replacement device includes: multiple reservoirs that are mounted on a well plate including multiple wells, which each contain a culture and a culture medium, and are provided respectively for the multiple wells and that each contain a culture medium to be supplied to a corresponding well; a gas supply pump that discharges a gas supplied to the multiple reservoirs; a gas supply passage unit that connects the gas supply pump and the multiple reservoirs and that transmits the gas from the gas supply pump to the multiple reservoirs; and multiple liquid supply passages that each connect a reservoir and a corresponding well and transmit, to the corresponding well, the culture medium pushed out from the reservoir by the gas flowing into the reservoir.

A pressure buffering system includes a housing, a pump module, a pressure sensor and a pressure cylinder. The pump module, the pressure sensor and the pressure cylinder are disposed in the housing. The pressure cylinder is communicated between the pump module and the pressure sensor. A biological culture device is further provided.

A high-throughput, three-dimensional microelectrode array for in vitro electrophysiological applications includes a 3D printed well plate having a top face and bottom face, and a plurality of culture wells formed on the top face of the well plate. Each culture well includes a plurality of vertical microchannels on the top face and microtroughs formed on the bottom face and communicating with the microchannels. A conductive paste fills the microtroughs and the microchannels and forms self-isolated microelectrodes in each culture well and conductive traces that communicate with the self-isolated microelectrodes.

A method and system of and for handling, preserving, separating, filtering, collecting, manipulating, and/or culturing ex vivo biological material including red blood cells, white blood cells, and blood plasma within a bioreactor having a gas permeable membrane that allows for passive ventilation.

The disclosure relates to multi-well plates having fluidic connections between neighboring wells that are useful to produce a cell culture substrate and compliant with American National Standards Institute of the Society for Laboratory Automation and Screening (ANSI/SLAS) microplate standards.