The present disclosure relates to optical fiber-based devices, and more particularly to light-absorbing optical fiber-based systems and methods.

Disclosed are a reaction tube for nucleic acid amplification capable of controlling a liquid circulation path, a reaction apparatus for nucleic acid amplification comprising the reaction tube, and a method for amplifying nucleic acid comprising a step of using the reaction tube. Also disclosed are a kit comprising the reaction tube, and use of the reaction tube in preparation of a kit.

Disclosed are a reaction tube for nucleic acid amplification capable of controlling a liquid circulation path, a reaction apparatus for nucleic acid amplification comprising the reaction tube, and a method for amplifying nucleic acid comprising a step of using the reaction tube. Also disclosed are a kit comprising the reaction tube, and use of the reaction tube in preparation of a kit.

In the present invention, information is analyzed, the positional relationship of cells/microbes in the optical axis direction is detected, and motility of cells/microbes is evaluated even in an out-of-focus view from an image obtained by a single image capture in an observation view of the cells/microbes. The present invention is provided with an optical system used to measure microparticles present in a sample liquid in a sample container, a drive mechanism for driving the sample container and/or a portion of the optical system in order to three-dimensionally search a bottom surface of the sample container, a control unit for controlling the optical system or the drive mechanism, an image processing unit for dividing an image of microparticles in the sample container at a first time and a second time into an in-focus region and an out-of-focus region and acquiring information relating to the microparticles, and a display unit for displaying the information relating to the microparticles as information representing a temporal change between the first time and the second time.

A culture apparatus including: a heat-insulated casing including an inner case surrounding a culture space, and an outer case, the heat-insulated casing having an opening; a box-shaped heat-insulated door to open and close the opening; and, one or more heaters provided to one or more inner surfaces of the inner case and an inner surface of an inner wall surface of the heat-insulated door, the inner wall surface facing the culture space when the door is closed; the heaters including one or more first heaters turned on when the interior of the culture space is at a first temperature to incubate a culture in the culture space and when the interior of the culture space is controlled at a second temperature to make the interior of the culture space, and second heaters to be energized when the interior of the culture space is controlled at the second temperature to sterilize the interior of the culture space.

A sensor includes a first electrode, a second electrode, a third electrode, a semiconductor film that connects the first electrode and the second electrode, and a solid electrolyte membrane that covers the first electrode, the second electrode, and the semiconductor film, in which the solid electrolyte membrane has an exposed surface exposed to an outside, and the third electrode is configured to be disposed at a position where when the exposed surface of the solid electrolyte membrane is in contact with a conductive liquid (Lq), it is possible to apply an electric field to the exposed surface of the solid electrolyte membrane through the conductive liquid (Lq).

The present invention provides a device for separating a single colony in a deep-sea in-situ environment. The device includes a separation operation incubator, a liquid injection unit, a sampling unit, and a sampling probe device. The present invention further provides a method for separating a single colony in a deep-sea in-situ environment. The method includes: under the condition of maintaining the pressure and temperature in the separation operation incubator consistent with those of a culture environment of deep-sea microorganisms, injecting a microbial bacterial liquid into the separation operation incubator through the liquid injection unit; carrying out dipping and streaking operations by the sampling probe device; then, carrying out separation and culture; and at last, selecting a single colony by the sampling unit to realize separation of a single colony.

A cell culture apparatus in which on an abutting surface (2a) around an opening part of a thermostatic chamber (3) and a cold storage chamber (4) that abuts against packing elements (3b, 4b) when front doors (3a, 4a) are closed, a communication groove (5) is provided that intercommunicates the inside and outside of the thermostatic chamber (3) by a communication groove (51) when a transfer tube (U40) is removed. The shut-off mechanism (5) is formed of: a guide hole (52) that opens on the abutting surface (2a) across the communication groove (51) and is deeper than the communication groove (51); a shutter member (53) which is inserted into the guide hole (52) and moves between the blocking position blocking the communication groove (51) and the opening position opening the communication groove (51); and a spring member (54) energizing the shutter member (53) to the blocking position.

A cell culture apparatus in which on an abutting surface (2a) around an opening part of a thermostatic chamber (3) and a cold storage chamber (4) that abuts against packing elements (3b, 4b) when front doors (3a, 4a) are closed, a communication groove (5) is provided that intercommunicates the inside and outside of the thermostatic chamber (3) by a communication groove (51) when a transfer tube (U40) is removed. The shut-off mechanism (5) is formed of: a guide hole (52) that opens on the abutting surface (2a) across the communication groove (51) and is deeper than the communication groove (51); a shutter member (53) which is inserted into the guide hole (52) and moves between the blocking position blocking the communication groove (51) and the opening position opening the communication groove (51); and a spring member (54) energizing the shutter member (53) to the blocking position.

The present disclosure provides a micro-fluidic chip and a reaction system. The micro-fluidic chip includes: a base substrate; a micro-cavity defining layer on the base substrate and defining a plurality of micro-reaction chambers; a cover plate on a side of the micro-cavity defining layer away from the base substrate; a heating layer disposed between the micro-cavity defining layer and one of the base substrate and the cover plate, and configured to heat the plurality of micro-reaction chambers. One of the base substrate and the cover plate, which is away from the heating layer, has a first surface and a second surface, the first surface faces the heating layer, the second surface faces away from the heating layer, and a area of the second surface is larger than that of the first surface.