The present invention provides a cell culture sheet comprising a plurality of recesses each having an opening of 1000 μm or less in diameter, wherein each recess has an inner circumferential face and a bottom face, wherein the inner circumferential face has a non-cell adhesive surface, and wherein the bottom face has a cell-adhesive surface; and a method for producing a cell culture sheet, the method comprising laminating of a layer having a non-cell adhesive surface and a layer having a cell-adhesive surface, wherein the layer having a non-cell adhesive surface has a plurality of through-holes of 1000 μm or less in diameter. The cell culture sheet of the present invention can be prepared simply and assists efficient cell culture operation, and therefore can preferably be used in the field of cell-based pharmaceutical preparations, for example, spheroid-containing pharmaceutical preparations etc.

A microalgae culturing method by using a paper-based culture apparatus, includes: 1) designing the shape of a culture apparatus as a rectangle with circles distributed in its middle; 2) spraying and printing wax on a piece of filter paper; 3) heating such that the wax penetrates through the filter paper to obtain a paper culture apparatus, where a surface of the filter paper on which the wax is sprayed and printed is called a surface A while the other surface thereof is called a surface B; and 4) dropping a first agar aqueous solution onto the circles on the surface B of the paper culture apparatus obtained in the step 3), and dropping a second agar aqueous solution added with microalgae onto the surface A of the paper culture apparatus obtained in the step 3).

Assemblies, systems, and methods for isolation of target material are provided. In various embodiments, an assembly for target material isolation includes a housing having an upper portion and a lower potion together defining an inner chamber. The inner chamber includes a semi-toroidal shape and the semi-toroidal shape defines a longitudinal axis. The assembly further includes one or more fluidic connection from the exterior of the housing to the inner chamber. An isolation material (e.g., polymer wool and/or magnetic beads) may be disposed within the inner chamber. A system includes a configured to fit at least a portion of the housing and releasably couple the assembly. Upon activation of the motor, the assembly may rotate about the longitudinal axis. An angle of the platform may be adjustable to thereby change the angle of the longitudinal axis about which the assembly rotates.

The present disclosure provides cell culture chambers for use in automated cell engineering systems, and in particular, cell culture chambers that include improved cell-contacting surfaces. Improved cell-contacting surfaces can include a surface coating that promotes cell growth, adherence, differentiation, maintenance of phenotype, and/or improves transduction; a cell-contacting surface comprising a non-porous, gas-permeable material; as well as other modifications to the cell-contacting surfaces. Cassettes comprising the cell culture chambers are also provided.

A laboratory container, in particular a cell culture container, includes a container volume which is surrounded by a container body and which can be accessed from the outside through a container opening of the container body. The container has a fluid line which communicates with the container volume and which comprises a valve arrangement. The fluid line is designed to conduct fluid in order to discharge and/or introduce fluid out of and/or into the container volume. The fluid line can be selectively released in order to conduct a fluid or blocked by the valve arrangement, and the container additionally has a gas balancing opening, through which gas can be introduced into or discharged out of the container volume in counterflow to a possible fluid flow in the fluid line. The gas compensating opening is surrounded by a channel wall, said line channel running from the gas balancing opening into the container volume and opening into said container volume.

The present invention relates to a novel bioreactor system for cell cultivation. More specifically, the invention relates to a compact bioreactor system which has several integrated functions and enables small scale static culture as well as scale-up rocking culture in the same bioreactor. The bioreactor system comprises tray for positioning of a cell culture bag having adjustable volume, a lid covering the cell culture bag and provided with heating function, an integrated perfusion unit, an integrated cell loading unit, and an integrated unit for automatic cell culture sampling, wherein the bioreactor system is controlled by a single control unit. The invention also relates to a method of cell culture using the bioreactor system for culture of therapeutic cells.

To continue a series of cell treatments even in a case where a treatment has become impossible in any isolator, a cell treatment system is provided with at least two isolators that perform different treatments, such that a sequential series of cell treatments is performed in each of the isolators. The cell treatment system is provided with a first isolator that performs a first treatment, a second isolator that performs a second treatment different from the first treatment, and a common isolator capable of performing the first treatment and the second treatment.

A remote monitoring system configured to non-invasively measure a cell culture is provided. The system includes a plurality of cell culture layers comprising a cell culture chamber configured to operate as a closed-system, the at least one cell culture chamber having at least one surface to which cells adhere. The system further includes at least one monitoring layer comprising an outer wall surrounding a monitoring layer cell culture chamber configured to operate as a closed-system and having at least one surface to which cells adhere, the at least one monitoring layer comprising at least one indentation in the outer wall. The system also includes at least one monitoring module disposed in at least one of the at least one indentation and comprising at least one of a confluence monitor and an analyte monitor.

A culture vessel that can suppress foaming in a culture medium when a common nozzle is used to supply a liquid culture medium and a gas together. This culture vessel includes: a vessel body having an opening portion that communicates with a housing space; a lid that closes the opening portion; and a nozzle that passes through the and extends to the housing space, and supplies a liquid culture medium and a gas together. The nozzle has a vent opening portion that is formed so as to open in a side of the nozzle and serves as a movement path of the gas from inside of the nozzle into the housing space.

A cell culture article comprises virgin polystyrene disposed over at least a portion of a surface of an article main body. The article main body may be formed from polyethylene terephthalate (PET) such as reground or recycled polyethylene terephthalate. Methods of making such a cell culture article include applying a composition to a surface of an article main body, where the composition comprises virgin polystyrene having a thickness of 1 to 500 microns and the article main body is formed from glass, ceramic, metal or polymer.