A thin film culture device for detecting yeast and mold microorganisms in a sample is provided. The culture device comprises a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, and an indicator agent for detecting a phosphatase enzyme activity, wherein each of the plurality of indicator agents comprises a detectable reporter group. A method of using the culture device is also provided.

The invention relates to a panel (100, 200, 300, 400) for a photobioreactor comprising at least: two plates assembled together, at least one of which is transparent and between which a lighting device is inserted; at least two openings allowing the passage of a fluid from a first face to a second face of the panel (100, 200, 300, 400) and characterized in that said luminous device is a fabric (101, 201, 202, 301, 401) including at least one optical fiber (2) capable of diffusing the light through said at least one transparent plate.

The present invention relates generally to tissue container systems that find use in the transport of tissues and methods of using the tissue container systems. In particular the present invention relates to systems that support the transport, thawing and use of cryopreserved human skin equivalents, and methods of their use by a health care provider.

The method for producing a three-dimensional artificial tissue is a method in which a three-dimensional artificial tissue extending in a predetermined direction is produced. The method includes: preparing a device including a culture tank having a culturing space surrounded by sidewalls, and a flow channel-forming member that penetrates through the sidewalls that face each other and is suspended in the culturing space along a predetermined direction; culturing cells in the culturing space and thereby forming a three-dimensional artificial tissue through which the flow channel-forming member penetrates; and removing the flow channel-forming member from the three-dimensional artificial tissue and thereby forming a perfusion flow channel that penetrates through the three-dimensional artificial tissue.

A microfluidic device includes a flow path through which a reaction solution flows, and an introducing portion for introducing the reaction solution into the flow path, wherein the flow path passes alternately and repeatedly several times through a first temperature region and a second temperature region having different predetermined temperatures, and the flow path includes the repeating unit portions passing through the first temperature region and the second temperature region, the repeating unit portions having decreasing lengths as the repeating unit portions are located farther away from the introducing portion.

A perfusion culture apparatus, capable of culturing cells having a multilayer structure, includes (i) a film, which is a sheet-shaped carrier on which cells are seeded in a state in which a liquid medium is allowed to pass through the sheet-shaped carrier; (ii) a vessel, which holds the film in a state in which the liquid medium is in contact with front and rear surfaces of the film; and (iii) a gas chamber, which applies pressure from outside to the liquid medium on the side in contact with either of the front and rear surfaces of the film to form a pressure difference between the liquid medium on the side in contact with the one surface and the liquid medium on the side in contact with the other surface, the liquid medium passing through the film in accordance with the pressure difference.

A gel tray for a bacterial transformation lab exercise has a plastic body with four parallel gel channels and four filling ports, one for each channel into which unmodified bacteria and heat-shocked bacteria can be injected by students along with appropriate reaction constituents to demonstrate transformation of the bacteria under visualization. A seal may be provided to seal the tops of the gel channels and a lid can cover the gel tray during incubation.

A gel tray for a bacteria transformation lab exercise has a transparent plastic body with four parallel gel channels and four filling ports, one for each channel into which unmodified bacteria and heat-shocked bacteria can be injected by students along with appropriate reaction constituents to demonstrate transformation of the bacteria under visualization.

Described herein is an improved automatic bioreactor system utilizing a multi-tray or multi-plate cell culture vessel for cultivating adherent cells.

The present invention concerns a module for a photobioreactor suitable for being assembled to an identical adjacent module, comprising: a panel having a first face and a second face, opposite the first face, the first face delimiting, with the second face of the adjacent panel, a cavity suitable for containing a culture medium when the modules are assembled, the cavity (14) having a thickness of between 1 millimetre and 2 centimetres, and a gas injection device, capable of injecting gas bubbles into the culture medium contained in the cavity, such that the bubbles rise along the faces of the panels.