A method and a device for adherent culture of microalgae in a thin liquid layer by mobile mechanical stirring, comprising: a subsystem for adherent culture of microalgae in thin liquid layer, for adherent culture of microalgae in a culture solution, comprising a microalgae liquid pool and a culture plate used as a base plate for microalgae seed inoculation, adherent culture, microalgae harvesting, the microalgae liquid pool containing culture solution having a water layer with a depth in a range of 1{tilde over ()}5 cm, forming a thin liquid layer; and a subsystem for mechanical stirring, comprising a mechanical stirring assembly, a moving assembly, a track member, a power drive assembly, a control assembly, the moving assembly moving on the track member to drive the mechanical stirring assembly to stir and suspend the microalgae, the microalgae standing and gradually settling to the bottom after stirring, realizing adherent culture in suspension and settlement cycle.

System and method for growing and harvesting algae. A plurality of growing trays containing growing medium for growing algae are arranged for flow of the growing medium sequentially therethrough. A holding tray configured to hold more growing medium than any one of the plurality of growing trays receives the growing medium from the plurality of growing trays. A pump draws the growing medium from the holding tray back into the plurality of growing trays.

A bioreactor includes a reservoir container for holding a liquid medium, a duct providing a flowpath in a generally vertical direction upward from the reservoir container, a plurality of fiber assemblies located within the duct, a top of which is higher than a top of the plurality of fiber assemblies, and a circulation system. The upper end of the duct comprises an overflow wall surrounded by a moat, a bottom of which is lower than a top of the overflow wall. The upper end of the duct and moat contact a pocket region that is bounded by a structure that is connected to the duct and that is isolated from fluid communication with an exterior of the pocket region. The liquid medium flows over the overflow wall within the pocket region, contacts gas in the pocket region, overflows into the moat and is removed therefrom by the circulation system.

Provided are in-vitro methods and devices for sustaining a synchronized circadian rhythm in cells of a cell culture by exposing the cells to a continuous flow of medium and to at least two stimuli provided in an oscillating manner with a periodicity of 244 hours, wherein a first stimulus and a second stimulus of said at least two stimuli are distinct, wherein said first stimulus is provided in a first time period and reaches a first peak in a first peak time period, and wherein a second stimulus is provided in a second time period and reaches a second peak in a second peak time period, and wherein an interval between end of time period of said first peak and beginning of said time period of said second peak is at least about 2 hours.

A system for culturing cells includes a bioreactor including a scaffold on which the cells tend to adhere. The system further includes a circulatory system that intermittently flows fluid over the scaffold.

A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

A bioreactor and method of forming complex three-dimensional tissue constructs in a single culture chamber. The bioreactor and methods may be used to form multi-phasic tissue constructs having tissue formed from multiple cell types in a single culture chamber. The bioreactor includes at least one translation mechanism to facilitate translation of one or more tissue constructs without direct user intervention, thereby providing a closed, sterile environment for complex tissue fabrication. The bioreactor may be used as a stand-alone device or as part of a large-scale system including many bioreactors. The large-scale system may include a perfusion system to monitor and regulate the tissue culture environment.

The present invention relates to systems and methods for tissue processing and analysis. Tissue chambers are configured to allow single-container chemical processing, imaging, and wax embedding of tissue samples in a single container without manipulation between steps. Tissue chambers with features to support the tissue sample and allow fluid flow between the tissue sample and the tissue chamber surface are disclosed. The features may be index matched to sample structures of interest or dissolvable in clearing solution to allow for in-chamber imaging with minimal distortion. Specialized tissue processing and wax removal apparatuses are also disclosed including for use with tissue chambers having frangible portions to permit ease of wax removal.

The invention provides an automated system for producing induced pluripotent stem cells (iPSCs) from adult somatic cells. Further, the system is used for producing differentiated adult cells from stem cells.

A micro electrode array (MEA) platform including a cell culture container configured to accommodate a cell culture medium to culture neurons, an MEA including an electrode configured to sense the neurons, and a temperature control device configured to control a transfer of heat generated by a heating source through a heating wire, based on whether a temperature of the cell culture medium or the electrode is equal to a reference temperature for the neurons.