The present invention is a cell cultivating vessel or device, such as a single or multitier flask, including a cover having a top plate, a side wall and a resealable port; an intermediate tray for receiving cells and cell culture media, having a bottom plate, a side wall, and a gap region formed between an interior upwardly angled lip located on an interior portion of the intermediate tray bottom plate and an adjacent outwardly angled side wall portion of the intermediate tray bottom plate, wherein the lip has a outwardly swooping curvilinear edge feature; and a base tray for receiving the cells and cell culture media, including a bottom plate and a side wall. The intermediate tray is positioned between the cover and the base tray, such that the gap region of the intermediate tray bottom plate is in alignment with the port located on the cover, resulting in the port, the intermediate tray and the base tray in fluid communication with one another which provides direct access, such as by a user to remove and/or add cells, cell media, and nutrients located on each of the intermediate and/or the base trays. Alternatively, the cell cultivating flask includes a plurality of intermediate trays stacked on top of one another and the gap regions of each intermediate tray are in alignment with each other and with the port on the cover.

Disclosed herein are standardized and optimized, modular single-use kits and methods for sterile or non-sterile preparation of biological material, such as cells or tissue, for immediate use, further preparation, or storage. These single-use kits and methods facilitate and improve a healthcare practitioner's ability to prepare biological material for research, diagnostic and therapeutic purposes in a convenient, consistent, repeatable, safe and effective manner.

The method for producing a three-dimensional artificial tissue is a method in which a three-dimensional artificial tissue extending in a predetermined direction is produced. The method includes: preparing a device including a culture tank having a culturing space surrounded by sidewalls, and a flow channel-forming member that penetrates through the sidewalls that face each other and is suspended in the culturing space along a predetermined direction; culturing cells in the culturing space and thereby forming a three-dimensional artificial tissue through which the flow channel-forming member penetrates; and removing the flow channel-forming member from the three-dimensional artificial tissue and thereby forming a perfusion flow channel that penetrates through the three-dimensional artificial tissue.

The present disclosure provides a culture device for enumerating colonies of sulfate-reducing microorganisms. The device includes a body having a waterproof base, a waterproof coversheet attached to the base, and a growth compartment disposed therebetween. The growth compartment has a perimeter and an opening. A portion of the perimeter is defined by a waterproof seal. The portion can include >50% of the perimeter. Disposed in the growth compartment are a dry cold water-soluble gelling agent, a dry culture medium selected to facilitate growth of a sulfate-reducing bacterium or indicator reagent for detecting hydrogen sulfide production by a sulfate-reducing bacterium, and a dry first oxygen-scavenging reagent.

A photobioreactor for cultivating photoautotrophs that comprises a first hatcher for containing the photoautotrophs and a first feed medium; a second hatcher for holding the photoautotrophs and a second feed medium; and a pump connected to the first hatcher, the second hatcher or both the first hatcher and the second hatcher for moving the photoautotrophs between the first hatcher and the second hatcher or vice versa.

A cell culture apparatus including cell culture units U and a measurement part (5). The measurement part (5) is provided with an imaging part (51) and is disposed so as to be capable of moving up and down between a back panel part (21) of a casing (2) and a culture container accommodating shelf (32). When the measurement unit (5) reaches a measurement position of each cell culture unit U which is a measurement subject, the culture container accommodating shelf (32) slides toward the measurement unit (5) so that portions to be measured of culture containers U20, U30 in each cell culture unit U come in an imaging range of the imaging part (51).

Described herein are bioreactor chambers and systems thereof. In an embodiment, a single-plate symmetrical bioreactor chamber is described, comprising: a flow channel extending along a first axis, wherein the flow channel comprises an inlet and an outlet at opposing ends of 5 the flow channel; a pair of struts on opposing ends of a second axis, wherein the second axis is substantially perpendicular to the first axis, wherein each strut of the pair of struts are placed on opposing sides of the flow channel, wherein the struts are configured to be coupled to a bidirectional linear actuator and configured to provide a strain perpendicular to a fluid flow through the flow channel, wherein the strain does not laterally displace cells present in the flow channel.

A system and method for creating mycelium textile materials is disclosed, in which leftover, cutoff, and other materials used to create the textile materials that would otherwise be discarded are further used in a second process to create bonded mycelium boards. The system and method further reuse or recycle materials throughout the process of creating the mycelium textile fabric and bonded mycelium boards, thus keeping waste to a minimum.

An intelligent bacteria collection system includes a bottle opening module and a peristaltic pump module opposite to each other, wherein a guide rail is arranged between the bottle opening module and the peristaltic pump module, and a tray module is placed on the guide rail; a manipulator module and the peristaltic pump module are fixed on an infusion operation platform; a control infusion module is placed on the tray module, which can be moved to and fixed on the infusion operation platform by the manipulator module. The present invention replaces the conventional artificial bacteria collection process, makes the bacteria collection process more rapid, reduces the possible secondary pollution in the manual detection operation process, improves the accuracy of the bacteria collection detection, and improves the work efficiency. The present invention provides high degree of automation, wherein no manual operation is required in the bacteria collection process.

A system for quickly determining antibiotic sensitivity of a microorganism comprising a triangular-shaped plate and cover for culturing, recovering, and re-suspending recovered microbial colonies and a second triangular-shaped plate comprising a non-liquid medium cut with concentric trenches over which the re-suspended microbial colonies are distributed by centrifugation and contacted with antimicrobial strips or disks.