A system for controlling a motion of a cell culture includes a tray adapted to hold a cell culture, a camera adapted to capture an image of the cell culture, and a device adapted to control a movement of the tray. The system also includes a processor adapted to determine a first movement of the tray, receive from the camera data representing an image of the cell culture, determine a characteristic of the cell culture based on the image data, determine a second movement of the tray based on the characteristic, the second movement being different from the first movement, and cause the tray to move in accordance with the second movement.

A multilayer culture vessel, comprising a culture vessel part and a reservoir part attached to the culture vessel part, wherein the culture vessel part comprises at least two culture trays that are stacked; the reservoir part comprises a surrounding wall defining an internal space and a port communicating with the internal space; the surrounding wall comprises a first surrounding wall part provided with the port and a second surrounding wall part facing the first surrounding wall part; the culture vessel part has openings communicating individual culture trays of the at least two culture trays with the internal space of the reservoir part; and the openings of the culture vessel part extend toward a first abutting portion that the first surrounding wall part abuts on the culture vessel part from a second abutting portion that the second surrounding wall part abuts on the culture vessel part.

A cell culture container includes a container main body comprising a gas-permeable plastic film, and an injection/discharge port. A peripheral portion of the container main body is sealed, and the container main body has a bulging shape on a top surface side of the container main body. A plurality of concave portions serving as cell culture parts are provided on a bottom surface of the container main body.

Devices for microbial detection of microorganisms are provided including a body member including a substrate having a first major surface and a second major surface. The device further includes a substantially dry, first microbial growth nutrient composition disposed on a portion of the first major surface, a first adhesive composition adhered to the first microbial growth nutrient composition, and a cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition. The device also includes a cover sheet attached to the body member, where the cover sheet includes a first major surface facing the body member. Devices including a water-proof pouch are also provided. Methods for detecting and enumerating at least one microorganism in a sample are additionally provided, using the devices.

The present invention relates generally to tissue container systems that find use in the transport of tissues and methods of using the tissue container systems. In particular the present invention relates to systems that support the transport, thawing and use of cryopreserved human skin equivalents, and methods of their use by a health care provider.

A device for growing microorganisms. The device includes a body member comprising a self-supporting, water-proof substrate having upper and lower surfaces; a hydrophobic spacer element adhered to the upper surface of the substrate forming side walls to retain a predetermined amount of liquid in contact with the substrate, wherein the hydrophobic spacer element has a hole therein; a fluid control film in the hole of the hydrophobic spacer element; a cover sheet having an inner-facing surface and an outer-facing surface, the cover sheet adhered to at least a portion of the body member; and a substantially dry, first microbial growth nutrient composition disposed on a portion of the inner surface of the cover sheet; a first adhesive composition adhered to the first microbial growth nutrient composition; and a cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition.

A method includes dispensing a liquid from a dispenser having a first piston channel and a first nozzle channel that is non-parallel to the first piston channel and is connected to the first piston channel at a first intersection. The dispenser includes a first piston configured to slide within the first piston channel; a first nozzle coupled with the first nozzle channel; a first valve located between a dispensing-liquid reservoir and the first intersection; and a second valve located between the first intersection and the first nozzle. Dispensing the liquid from the first dispenser includes pulling the first piston to initiate flow of a liquid in the dispensing-liquid reservoir to the first intersection; and subsequent to pulling the first piston, pushing the first piston to initiate flow of the liquid in the first intersection to the first nozzle so that the liquid is dispensed from the first nozzle.

A bioartificial liver (BAL) based on human induced pluripotent stem cells (iPSCs)-derived hepatocyte-like cells (HLCs) and a multilayer porous bioreactor is provided. The plasma separation/retransfusion loop part includes a blood input pipe, an exhaust pipe spring clamp, a blood input peristaltic pump, a heparin pump, a plasma separation column, a first pressure monitor, and a heater. The cell reactor/plasma component exchange double-loop part includes a plasma input peristaltic pump, and a semipermeable membrane exchange column, a plasma exchange peristaltic pump, a red blood cell (RBC) pool, a membrane lung, a multilayer porous bioreactor, a second pressure monitor, and a third pressure monitor arranged in a 37° C. dedicated incubator. An outlet of the third pressure monitor and a blood cell outlet are connected to an inlet of the first pressure monitor, and then connected to the heater and a blood output pipe in sequence.

An energy-saving and environment-friendly garbage disposal box capable of dispersing garbage, applicable to various areas, includes a domestic garbage disposal unit, a recyclable garbage disposal unit and a plastic garbage disposal unit. A pedal mashing device is provided to a rear portion of the domestic garbage disposal unit. The garbage disposal box is covered by solar panels and a computer display. An auto electronic weighing plate is provided to top the garbage disposal box. A set of mashing cutters is disposed inside the garbage disposal box to connect spatially with a fermentation area, and an organic fertilizer area is located under the fermentation area. The recyclable garbage disposal unit is furnished with a garbage entrance at an upper portion thereof and three external drawers corresponding to three recycle chambers. The plastic garbage disposal unit covered by solar panels is furnished thereinside a gravity compression iron and a battery set. The garbage disposal box provided by this patent disclosure and featured in flexible garbage dispersing functions can resolve environmental degradation caused by garbage piling and environmental pollution caused by the current garbage disposal means, and can also provide a possibility to maximize the garbage utilization rate.

A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.