A perfusion plate that can be combined with pillar plates containing cell layers is disclosed. The perfusion plate can have an inflow reservoir and an outflow reservoir connected by at least one channel, which fluidly connects the perfusion wells to the reservoirs for the flow of a fluid such as growth media. A perfusion plate can be part of an assembly containing a pillar plate, a lid, and a transparent bottom for visualizing cell growth in the perfusion wells. The perfusion-pillar plate assembly can facilitate perfusion-based tissue culture and tissue communication for high throughput, high-content, drug screening.

Harvesting methods and systems for harvesting fungal biomass from a growth container using one or more meshes is described. The growth container includes a first mesh through which the fungal biomass grows. A second mesh can be disposed over the first mesh such that the fungal biomass grows further through the second mesh. A rotator or a slider can be coupled to the second mesh and configured to move the second mesh relative to the first mesh causing the fungal biomass to shear and transport the fungal biomass on the second mesh to a delivery area.

Growth and harvesting methods and systems herein include a growth container with one or more meshes attached thereto. The growth container and its meshes can hold a substrate. The growth container and its meshes can be vertically oriented to allow fungal biomass (e.g., mycelium, fruiting body, primordia, etc.) to grow from the substrate and through the meshes. The growth containers can be cylindrical or a rectangular box having meshes extending along vertically oriented longitudinal sides. The meshes may be provided on two opposite sides of the growth cylinders. These meshes can expose the substrate to a growth environment to facilitate growth of the fungal biomass. A cutter is configured to move relative to the growth container to harvest the fungal biomass across the mesh while preventing the substrate from sticking to the harvested fungal biomass.

An animal-derived bacteria accelerated culture device includes: a bacteria accelerated incubator; support fixed feet installed on a lower end of the bacteria accelerated incubator; limit fixed frames installed on an end of the bacteria accelerated incubator facing away from the support fixed feet, each the limit fixed frame being provided with a limit fixed groove and a first fixed pin hole; a detection frame installed on an upper end of the bacteria accelerated incubator. By controlling working detection heads to detect bacteria inside the bacteria accelerated incubator, detection results are transmitted to a processing work case through detection positioning brackets for analysis and processing, and data is displayed by a data display device, the staff can adjust parameters such as temperature, potential of hydrogen (pH), oxygen, nutrients, etc. of the bacteria accelerated incubator through displayed values, thus improving the work quality and efficiency.

Embodiments of the present invention provide an automatic micro algae culturing device and system for self-cleaning, continual automatic algae culturing and irradiant optimizing. The culturing device includes a photosynthesis tubular reactor for micro algae culturing, with transparent cleaning particles to scrape off any unwanted particles or components such as including and not limited to formation of biofilm. The device also includes a nano-air bubble generating device installed around the tubular reactor to generate and feed nano-air bubbles into the tubular reactor, where the nano-air bubbles increase an active surface area for ions to interact with the algae in the culture medium in order to increase productivity of the culturing device by enhancing rate of mitosis, productively of oil, protein and polysaccharide and the nano-air bubbles also sterilizes the culture medium.

A culture vessel that can suppress foaming in a culture medium when a common nozzle is used to supply a liquid culture medium and a gas together. This culture vessel includes: a vessel body having an opening portion that communicates with a housing space; a lid that closes the opening portion; and a nozzle that passes through the and extends to the housing space, and supplies a liquid culture medium and a gas together. The nozzle has a vent opening portion that is formed so as to open in a side of the nozzle and serves as a movement path of the gas from inside of the nozzle into the housing space.

There is provided a connector, for introducing or extracting a material to or from at least one receptacle, comprising a housing extending between a distal end and a proximal end, the housing comprising, at least at one end, a pierceable seal; a hollow needle mounted, at least partially, within the housing between the distal end and the proximal end of the housing, a first end of the hollow needle being connected or connectable to a first corresponding receptacle, and a second end of the hollow needle facing the pierceable seal at an end of the housing; and an actuating mechanism acting on the housing or the hollow needle to enable the hollow needle to pierce the pierceable seal thereby forming a communication through the pierceable seal, such that material is able to transfer through the connector.

Microalgae cultivation equipment for the cultivation of microalgae is provided in which a raceway is modified so as to contain multiple generally upright photobioreactor columns spaced apart along its length so as to increase the total surface area of liquid growth medium directly exposed to light and to improve the transfer of CO.sub.2 from the gas-phase to the liquid-phase by providing adequate height inside the vertical photobioreactor columns. The lowermost ends of the photobioreactor columns are immersed inside the liquid growth medium in the raceway component and are fed with liquid growth medium by a circulation promoting facility circulating the liquid growth medium from the raceway through the photobioreactor columns to become discharged back into the raceway. Gas inlets provide CO.sub.2 containing gas bubbles passing upwards in each of the photobioreactor columns. One or more paddle wheels or jet pumps induce a flow of liquid growth medium within the raceway.

The present disclosure describes a manufacturing method to use algae as a renewable green factory for producing biodegradable bioplastic. One or more embodiments include separation of a cultivated microalgae biomass from water before use in the wet or dried state. The lipids and proteins are extracted from the biomass which leaves starch and algae precursors in the remaining material from the microalgae cells. The starch includes amylose, amylopectin, monosaccharides kinases and cyclobutadiene and is hydrolyzed into a syrup containing oligosaccharides and polysaccharides. In some cases, the syrup is used as an ingredient in a medium containing nutrient for bacterial fermentation of plastics.

An insect culture maintenance system includes a sequence of open-ended cylindrical tubes [500, 502, 504, 506] joined pairwise alternately at their tops and bottoms using multiple dual-cap connectors. Each dual-capped connector has a channel from an inside of a first cap to an inside of a second cap. In use, connectors that cap the bottoms of the tubes [508, 512] are filled with insect food media [518, 520], while connectors that cap the tops of the tubes [510] are open. As a result of this design, adults pass from one tube to the next through the top dual-cap connectors, while larvae pass from one tube to the next through the bottom dual-cap connectors, resulting in propagation of subsequent generations of insects through the sequence of tubes.