A biological indicator for testing the efficacy of sterilization or disinfection processes of closed environments or confined spaces, the biological indicator comprising a carrier; biological material supported on or embedded in the carrier; and a carrier container comprising a body and a cap. The carrier is fixed within an inner space of the cap, the cap being able to be placed in an open position completely exposing the carrier and the biological material to the closed environment or confined space to be disinfected. A method for determine the efficacy of a process of sterilization or disinfection of closed environments or confined spaces, preferably by means of an enzyme and a fluorescence compound, using said biological indicator.

A photobioreactor with a microalgae's cultivation chamber in the form of a duct that is constructed from an inflatable sleeve from transparent polymer film; this inflatable sleeve excluding its terminal sections is sandwiched between a bank of frames with wire nettings from below and glass panes from above the inflatable sleeve, and it has a small inclination regarding the horizontal plane. The terminal sections of the inflatable sleeve are in fluid communication with two headers, which are used for supplying and removal of gaseous medium and suspension of microalgae into and out of the duct.

A cell culture system including self-propelled transport device for transporting cells or materials to be used for culture operation, treatment units for performing culture operation using the cells and materials and a transport command unit for controlling the self-propelled transport device to move the cells and materials to a required treatment unit. To control the self-propelled transport device, the transport command unit uses cell driving parameters when the cells are moved, and uses material driving parameters when the materials are moved. Hence, transport of cells and materials using the self-propelled transport device can be optimized, and culture operation can be performed efficiently.

The present disclosure relates to a culture medium having an agar gel forming by at least one plant growth nutrient, agar powder and water, wherein the agar gel is 100 wt %. A culture medium manufacturing method, a culture medium structure and a culture medium structure usage method are also illustrated in the present disclosure. The culture medium structure has a culture medium of the above culture medium, a seeding board for receiving the culture medium layer, wherein the seeding board has a hollow body which has a top hole, a bottom hole opposite to the top hole and a top base disposed on a top peripheral of the hollow body, and the hollow body and the top base are integrally formed. Via the culture medium of the solid agar gel having the plant growth nutrient, growth health and quality of crops are enhanced.

Provided are cell culture apparatus and method. The cell culture apparatus comprises a culture column and a perfusion system, the culture column comprising a first and a second tube-connecting caps and a support for cells to attach and grow thereon. The first and the second tube-connecting caps are respectively provided with a perfusion inlet and a perfusion outlet; The perfusion system comprises a culture medium storage device, a perfusion tube and a perfusion pump. A complete and closed culture column is formed in the cell culture device after the cells are inoculated, and the culturing is completed by the perfusion system to avoid contamination.

An isolation device for adipose-derived stromal vascular fraction is provided. More particularly the embodiments relates to novel systems, devices, methods and kits for adipose tissue collection and stromal vascular fractions isolation from collected adipose. The devices and systems are used in the field of healthcare/regenerative medicine.

The apparatuses described herein relate to preparation of a meat product. Apparatuses, systems comprising the apparatuses, and methods of making and use the systems and apparatuses are described herein. These are useful for controlling one or more of growth on and separation of a meat product from an enclosed substrate. The apparatuses and systems are configured to receive fluid and grow the meat product and/or separate the meat product from the substrate in a scalable manner.

Embodiments of the present invention may provide fluid flow coordinators, passive flow field modifiers, or even inwardly protruding helical spines which can be used in continuous, scalable, low energy usage, bioreactor systems perhaps to provide optimal mixing of microorganisms with nutrients, gases, or the like or even to move microorganisms, such as algae, in and out of light for effective and optimal growth.

A rapid phytoremediation reactor apparatus includes a tube, and the tube's interior has a rough texture configured for supporting growth of algae. A method for cultivating algae includes flowing a liquid through a tube with periphyton growing on an inner surface of the tube, the tube being penetrable to light; illuminating the tube to grow the periphyton on the inner surface of the tube; and harvesting the periphyton.

The microorganism preparation feeding method of the invention employs an automatic microorganism preparation feeding apparatus which includes a cold storage apparatus for refrigeration-storing a seed microorganism belonging to the aerobic microorganism group including at least one species of aerobic microorganisms capable of decomposing oil and fat contained in oil/fat-including wastewater and a growth tank for growing the seed microorganism so as to produce the microorganism preparation, wherein the seed microorganism belonging to the aerobic microorganism group is maintained in a live state by means of the cold storage apparatus, the seed microorganism is periodically grown by means of the growth tank so as to produce a predetermined microorganism preparation, and the produced predetermined microorganism preparation is fed to the oil/fat-including wastewater. The method includes refrigeration-storing, as the seed microorganism, a microorganism whose population density is 1×10.sup.7 CFU/mL to 5×10.sup.9 CFU/mL in the cold storage apparatus; growing, as a source material, the seed microorganism of a predetermined volume by means of the growth tank so as to produce the predetermined microorganism preparation whose volume is 50 to 500 times the predetermined volume of the seed microorganism and whose population density is 1×10.sup.7 CFU/mL to 2×10.sup.10 CFU/mL; and feeding the produced microorganism preparation to the oil/fat-including wastewater.