Disclosed is a process for growing adherent cells in a containment box of a multi-parallel bioreactor, including: seeding the adherent cells on a carrier held in a culture dish; transferring the adherent cells on the carrier to a containment box of the multi-parallel bioreactor; and growing the adherent cells at a containment box while agitating the media at an impeller speed between 200 rpm to a 1200 rpm.

A plurality of caps are provided each with a visual coding of color and/or indicia for use with different sizes of bottles at least in accordance with a height of the bottles from a closed bottom end to an open top end for engaging each of the caps. Each of the caps has a tube mounted to, or through, an aperture extending through a top closed end of the cap to extend a selected length enabling one end of the tube to reach or extend along an interior surface of the bottom end of any of the bottles of the height to which the cap is coded for use therewith. The other end of tube is extendable to a bioreactor container. Each of the caps has another tube or port to another aperture of the cap to provide an air vent with an optional air filtering device.

The invention relates to a cell culture bottle for adherent cells (e.g. human mesenchymal stem cells), comprising: a vessel; an internal cylinder, which has an internal Archimedes screw; an internal central tube, through which liquid can flow; and at least one wall arranged around the central tube. This arrangement provides an enlarged inner surface for the growth of the cells and for the reliable mixing of the fluid. The cell culture bottle is formed as a single piece and can be simply and economically produced as a disposable device by means of additive manufacturing.

A cell culture vessel (400) includes a base defining a base plane extending in a first direction and a second direction perpendicular to the first direction, a plurality of cell culture chambers (200) stacked one atop another, each cell culture chamber having a top (202), a bottom (201) and sidewalls (203), each of the top, bottom and sidewalls having an interior surface, wherein at least the bottom surface has an array of microcavities (420) supporting the culture of cells as spheroids and each bottom surface is at an angle with respect to the plane of a table or surface upon which the vessel sits. Further, liquid can flow into each cell culture chamber via an inlet (210) and out of each cell culture chamber via an outlet (215). The angled cell culture surface allows the cell culture chambers to be perfused or allows media changes without dislodging spheroids from microcavities.

A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.

A cell culture vessel includes a vessel body, support columns, and a stabilizer device. The vessel body defines a cell culture chamber enclosed between a bottom wall and a top wall. The support column is within the cell culture chamber and extends between the top wall and the bottom wall. The stabilizer device covers a width and length of the cell culture chamber and has a column engaging structure that is sized to slidingly engage the support column such that the stabilizer device is movable along the support column as a liquid culture medium is received in the cell culture chamber. The support column guides the stabilizer device along a length of the support column as the stabilizer device rises with rising liquid level in the cell culture chamber during a liquid culture medium filling operation.

A method for carrying out a reaction process, in particular for setting the mixing and/or aeration of a reaction liquid while the reaction process is being carried out, which includes filling at least one reaction vessel with at least one reaction liquid, wherein an internal volume of the reaction vessel is not completely filled by the at least one reaction liquid at all times during the reaction process, and changing the internal volume of the reaction vessel in the course of the reaction process, in particular in a targeted manner, which causes a movement of the at least one reaction liquid.

A continuous automated perfusion culture analysis system (CAPCAS) comprises one or more fluidic systems configured to operate large numbers of biodevices in parallel. Each fluidic system comprises an input reservoir plate for receiving media; a biodevice plate comprising an array of biodevices fluidically coupled to the input reservoir plate, configured such that each biodevice has independent media delivery, fluid removal, stirring, and gas control, and each biodevice is capable of continuously receiving the media from the input reservoir plate; and an output plate fluidically coupled to the biodevice plate for real-time analysis and sampling. The operations of the CAPCAS are automated and computer-controlled wirelessly. The CAPCAS can also be used for abiotic and biotic chemical synthesis processes.

The application provides a system for continuous cell production, comprising: a culture container; and a polymer blended layer arranged on the inner surface of the culture container; wherein, the polymer blended layer is a pH-responsive polymer blended with nylon. Additionally, a method for continuous cell production using the system of the present application is provided.