The present invention relates to a device for cultivating cells, in particular tissue, comprising a carrier plate unit which has a central axis of rotation, at least one access opening arranged proximally to the axis of rotation, at least one cultivation chamber arranged distally to the axis of rotation, and at least one channel connecting the access opening to the cultivation chamber, and also a method for cultivating cells in a device according to the invention and a method for producing the device according to the invention.

The disclosure relates to a method of culturing and analyzing at least one cell in a microchamber configured to allow for optical inspection of the at least one cell, wherein liquid is extracted from the microchamber for analysis, characterized in that the analysis returns information about particles secreted from the at least one cell and that this information can be correlated to the individual cell and/or cell population. The disclosure further relates to a device for use in the method and a system and a computer program for performing one or more of the steps of the method.

A system, configured to facilitate processing and detection of nucleic acids, the system comprising a process fluid container and a cartridge comprising: a top layer, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a detection chamber, comprises a portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region.

In biosciences and related fields, it can be useful to study cells in isolation so that cells having unique and desirable properties can be identified within a heterogenous mixture of cells. Processes and methods disclosed herein provide for encapsulating cells within a microfluidic device and assaying the encapsulated cells. Encapsulation can, among other benefits, facilitate analyses of cells that generate secretions of interest which would otherwise rapidly diffuse away or mix with the secretions of other cells.

Systems and methods are provided to assist in developing a precision medicine approach using a microfluidic cell having a releasable, aqueous interfacial film to separate tissue channels. The approach can include a personalized medicine treatment plan. The systems and methods emulate cellular communication in a disease state in a more accurate aqueous environment and provide data on the interaction between the cells that can be used to develop a treatment for a subject in need. The systems and methods also can be used to assess the effect of a particular treatment, such as a drug therapy, radiation therapy, or a combination thereof, for example. The systems and methods can show how a particular therapy is affected by any of several known factors including, but not limited to, the sex of the subject, the age of the subject, hereditary factors or other genetic predispositions, as well as perhaps other physiological states of the subject, or a combination thereof.

The present disclosure relates to a herringbone-type fluid guiding unit and an apparatus for concentrating fluid using same. The herring-bone type fluid guiding unit includes: a front member formed on a flow path and formed so that the width between the left side and the right side widens from a front end part toward the back, with respect to the flow direction of a fluid; and a rear member extending from the front member toward the back, wherein the rear member is provided with a recessed part that is recessed to a specific depth from the rear edge toward the front or with a protruding part that protrudes toward the back.

The present invention provides devices that replicate tumor microenvironments in a microfluidic chip. The devices can be used to model certain disease states related to tumor microenvironments. The devices can be adapted to replicate tumor microenvironments from patient-specific cells such that treatment conditions can be modeled and tailored to individual patients. In some embodiments, the devices are suitable for evaluating cancer therapies on a patient-specific basis.

The present disclosure is drawn to microfluidic cellular membrane modification devices. In one example, a microfluidic cellular membrane modification device can include a microfluidic channel including a pumping portion and an electric field portion. An electrode pair can be positioned about the electric field portion. A bidirectional pump can be in fluid communication with the microfluidic channel at the pumping portion to move fluid backward and forward through the electric field portion.

The present disclosure relates to a method for micropatterning on silicone-based elastomer, the method including forming an initiator at a position of the silicone-based elastomer having high optical transmittance and transparency, and moving a laser beam to induce chain pyrolysis, thereby forming micropatterns with high quality in a very short time.

A microfluidic device comprises a cell flow layer and a control layer. The cell flow layer includes a growth channel, a collection channel, a plurality of bridge channels connecting the growth channel and the collection channel, a plurality of bridge valve portions, and a plurality of cell growth trenches coupled to the growth channel. The growth channel includes an inlet valve portion and an outlet valve portion controlling flow into and out of the growth channel. The collection channel includes an inlet valve portion and an outlet valve controlling flow into and out of the collection channel. The bridge valve portions control flow between the growth channel and the collection channel. The control layer includes a first control channel actuating the bridge valve portions and a second control channel actuating the inlet valve portions and the outlet valve portions of the growth channel and the collection channel.