The present disclosure provides a three-dimensional cell culture method and a cell culture apparatus using the same. The cell culture method includes lowering, when culturing cells on at least one surface of a porous support, a concentration of a cell-secreted substance in a lower region of the other surface of the porous support. The cell culture apparatus includes: an upper chamber including an opening, porous support having at least one surface on which cells are cultured, and a cell culture space in which a culture medium is housed; a lower chamber including a space in which at least one upper chamber is disposed; and a device lowering a concentration of a cell-secreted substance in a lower region of the other surface of the porous support.

High capacity, low cost devices for use in growing monocultures of novel, previously unknown microbial species contain adhesive layers with wells covered by nanoporous membranes and a membrane support layer that aids in membrane removal for access to growth chambers without contamination. The devices are placed in natural environments for cultivation of unknown microbial species.

A cell culture method and a cell culture device used with the cell culture method allow mass-producing a three-layer blood-brain barrier (BBB) in vitro model including a vascular endothelial cell, a pericyte, and an astrocyte. A cell culture method enhances mass production of the three-layer BBB models. A cell culture method includes injecting a suspended cell culture medium into an outer surface of a porous membrane in a cell culture insert, seeding the cell culture medium with an astrocyte to culture the astrocyte, seeding the cell culture insert being inverted with a pericyte to culture the pericyte, seeding the cell culture insert with a temperature-sensitive vascular endothelial cell embedded in a temperature-sensitive gel to culture the vascular endothelial cell, and melting the temperature-sensitive gel.

A cell culture system includes: a cell culture container disposed in an anaerobic chamber; and an external apparatus. The external apparatus has a main body unit disposed in the anaerobic chamber and an operation unit disposed outside the anaerobic chamber. The main body unit is configured to perform a predetermined process onto the cell culture container. The operation unit is configured to transmit or receive a signal associated with the predetermined process to or from the main body unit by wireless communication.

A fluidic device for culturing cells includes a microplate and plate lid. The microplate includes multiple wells and channels, the channels extending between the wells such that the channels interconnect the wells. The plate lid releasably engages the microplate to thereby enclose the wells and the channels. The wells include a culture surface such that a cell culture medium received therein is deposited over the culture surface. At least one channel that extends between adjacent ones of the wells is spaced from the culture surfaces of the adjacent wells defining a gap between the at least one channel and the culture surfaces of the adjacent wells for collection of the cell culture medium.

Provided herein are microtiter plates, systems, and uses thereof. In particular, provided herein are cell culture (e.g., microtiter plates) with a plurality of wells that comprise diffusion channels, systems comprising the devices (e.g., comprising cells), and use of the devices and systems in co-culture applications (e.g. in high throughput screening) and biological assays.

Disclosed herein are systems and methods relating to in-situ servo-hydraulic biomanipulators. In-situ servo-hydraulic bio-manipulators as described herein have the advantages over existing systems and methods at least by having a lower cost, interchangeable and/or disposal displacement devices coupled to the extrusion head, and mounting of the displacement head along the optical axis of a microscope for enhanced visibility and well clearance.

Disclosed is a well insert for cell culture and a method of manufacturing same, where the well insert has a membrane support made of a polyolefin material and having an upper end and a lower end, the upper end being adapted to engage a well of a microplate so as to suspend the well insert therein, and a permeable membrane sealed to the membrane for supporting a tissue culture, the permeable membrane being of brittle material and transparent in both air and water.

A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.

A cell culture method includes: a preparation step of preparing a cell culture membrane that includes (i) a membrane body formed of a thermosetting resin and including a first surface and a second surface opposite the first surface and (ii) a plurality of through pores formed in the membrane body and penetrating from the first surface to the second surface; and a culture step of seeding and culturing cells on each of the first surface and the second surface of the cell culture membrane prepared, in the through pores, a first average pore diameter in the first surface is smaller than a second average pore diameter in the second surface and the pore density of the through pores is equal to or less than 2.0×10.sup.5 pores/cm.sup.2.