The invention provides a fluidic device that comprises at least two separate testing units, each of which is adapted to expose living cells to a moving fluid. The fluidic devices are useful for testing cell types such as kidney cells that are sensitive to shear stress, and can be configured for high-throughput testing. The fluidic device is adapted to receive a multi-well cell culture plate to which the living cells can be adhered or affixed. In some embodiments, flow channels in the fluidic device are positioned to expose the living cells to moving fluid, and the flow wells are adapted to provide substantially uniform shear stress across the area where the living cells are exposed to the moving fluid.

The methods and systems described herein provide a cell culture platform with an array of tissue modeling environments and dynamic control of fluid flow. The cell culture platform includes an array of wells that are fluidically coupled by microchannel structures. The dynamically controlled flow of fluid interacts with cells grown within the microchannels.

An integrated modular microphysiological system is provided which includes a two or more chambers and a vascular network which includes at least one channel. The chamber can be configured for culturing a tissue and includes a layer of endothelial cells which forms an endothelial barrier within the well. The endothelial barrier can be in fluid contact with at least one of the at least one channels in the vascular network. The endothelial barrier can also be in fluid contact with a fluid in the chamber.

An apparatus for collecting or culturing cells or cell colonies includes: a common substrate formed from a flexible resilient polymeric material and having a plurality of wells formed therein; and a plurality of rigid cell carriers releasably connected to said common substrate, with said carriers arranged in the form of an array, and with each of the carriers resiliently received in one of the wells. A method of collecting or culturing cells or cell colonies with such an apparatus is carried out by depositing a liquid media carrying cells on the apparatus so that said cells settle on or adhere to said the carriers; and then (c) releasing at least one selected carrier having said cells thereon by gradual application of release energy to each carrier from the cavity in which it is received (e.g., by pushing with a probe).

Provided is a culture system comprising a base comprising a multiwell plate; a transwell-like type insert plate comprising a plurality of culture insert assemblies, wherein each culture insert assembly comprises a culture surface element that is removably mounted to a culture insert body; and a cover designed to fit over the transwell-like type insert plate.

The present disclosure comprises a lymphovascular bioreactor system and methods of making and using same.

The present invention relates to an insertable culture container and a kit for three-dimensional cell culture, and a three-dimensional cell co-culture method using the same, the insertable culture container for three-dimensional cell culture comprising: a cylindrical side wall having open upper and lower portions; at least one hook protruding outward from the upper side of the side wall; and at least one support protruding inward from the lower side of the side wall. The present invention is advantageous in that air required for a three-dimensional cell culture structure can be smoothly supplied since the cell is cultured at a position spaced apart from a bottom surface of the culture container, and an existing culture plate can be used without change due to the culture container configured as an insert type.

A packed-bed bioreactor system for culturing cells is provided, the system including a cell culture vessel having at least one interior reservoir, an inlet fluidly connected to the reservoir, and an outlet fluidly connected to the reservoir; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined multi-layered substrate for adhering cells thereto, and each layer of the multi-layered substrate has a physical structure and a porosity that are substantially regular and uniform.

One aspect of the invention provides a multichamber bioreactor. The multichamber bioreactor includes multiple planar layers stacked on each other defining at least one chamber and a clamping mechanism. The clamping mechanism includes a housing and retaining means received in the housing and configured to generate a controlled and uniform pressure to secure the stacked multiple planar layers in the housing. Each chamber is implemented from a separate fluidic layer, with each fluidic layer having ports and valves independent of the other layers. The micro fluidic ports can be actuated through a micro fluidic interconnect system utilizing rotary cylinder valves.

System and methods for the culturing of multiple independent cell types under uniform conditions.