The present invention provides a medical analysis device and a cell analysis method, which can capture many types of cancer cells, including cancer cells not expressing EpCAM. The present invention relates to a medical analysis device having a well portion, the well portion having a hydrophilic polymer layer formed at least partly on the inner surface thereof, the hydrophilic polymer layer having fibronectin adsorbed thereto.

Disclosed herein are devices, apparatuses and methods for generating self-sustaining gaseous and non-gaseous gradients across a cell support structure and for culturing one or more cells or tissues under hypoxic conditions. Methods of generating one or more gas gradients across a cell support structure include providing a luminal container having a bottom wall that is a gas permeable membrane, positioning a cell support structure above the bottom wall, positioning one or more cells or tissues on the cell support structure, and generating one or more gas gradients between the bottom wall, across the cell support structure and into a luminal reservoir. An apparatus to produce hypoxic conditions for cell cultures includes a luminal container having a bottom wall, a cell support structure on the bottom wall, and a cover that sealably closes the open top, such that a hypoxic condition can be generated in the luminal reservoir.

The present disclosure provides a device for detecting a tomato pathogenic fungus, the device including an artificial cell wall, a test-sample-solution receptacle disposed over the artificial cell wall, and a culture-solution storage disposed under the artificial cell wall, wherein, in the culture-solution storage, a culture solution contains greater than or equal to 0.5 mg/L and less than or equal to 1.3 mg/L of fludioxonil.

A system and method for studying cell injury mechanisms by applying biologically relevant mechanical impact to in vitro cell culture are disclosed. This approach is for maintaining consistent in vitro conditions during experiments, accommodating multiple cell populations, and monitoring each in real-time while achieving amplitude and time scale of input acceleration that mimic blunt injury cases. These multiplexed, environmental control capabilities enable characterizing the relationships between mechanical impact and cell injury in multivariate biological systems.

The biological detection cartridge includes a sample chamber having a port, plural culture chambers for incubating the sample therein, a channel system configured to deliver the sample into each of the culture chambers, plural quantitative chambers, and plural concave structures. The channel system includes a curved channel and plural inlet channels, the curved channel is communicated with the sample chamber, and each inlet channel is communicated with the curved channel and a corresponding culture chamber. Each quantitative chamber is disposed between a corresponding inlet channel and a corresponding culture chamber. Each concave structure is disposed between a corresponding quantitative chamber and a corresponding culture chamber. The concave structure includes a first hole disposed close to the culture chamber.

A bioreactor for the in vitro culture of reproductive tissues and the like in perifusion mode, including a first component and a second component, mutually connected, which define between them at least one culture chamber; the former component includes an inlet port for introducing a culture medium into the culture chamber, while the latter component includes an outlet port, to allow the culture medium out of the culture chamber. The bioreactor includes at least one porous medium, provided inside the culture chamber, able to mechanically support fragments of reproductive tissue, and networks, positioned above and below the fragments of reproductive tissue, with reference to the vertical or substantially vertical position of use of the bioreactor.

A microfluidic cell culture chip which contains a central module comprising a central unit, which contains a support consisting of a non-resorbable membrane, a 3D nanostructured porous membrane, comprising at least one protuberance, and the 3D nanostructured porous membrane and that at least one protuberance being composed of materials suitable for the culture of two distinct cell types; a base, and the central unit being integrated in the base, and forming a whole with the base.

A packed-bed bioreactor system for culturing cells is provided, the system including a cell culture vessel having at least one interior reservoir, an inlet fluidly connected to the reservoir, and an outlet fluidly connected to the reservoir; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined multi-layered substrate for adhering cells thereto, and each layer of the multi-layered substrate has a physical structure and a porosity that are substantially regular and uniform.

Disclosed is a method for preparing an implant including a culture of cells on a membrane, the method including steps that consist of: securing the membrane to a mounting; placing the membrane in a recess of a housing providing two spaces having adjusted heights, above and below the membrane; injecting, into the spaces, a liquid capable of transforming into gel at a transport temperature lower than an injection temperature of the liquid; and bringing the housing to the transport temperature, so as to form an implant including the membrane and two layers of gel having adjusted thicknesses, on two opposite faces of the membrane, respectively.

A cell culture vessel according to the present invention is characterized by comprising a first vessel having a stirring member disposed therein, and a second vessel configured to be inserted in the interior of the first vessel and having a hole for allowing a solvent accommodated in the first vessel to pass through the interior thereof, wherein the stirring member is arranged in a recess portion formed in the first vessel.