Provided are an alginate gel fiber for antibody production wherein an antibody-producing cell (for example, an antibody-producing CHO cell) is comprised in a core layer, and an antibody production method using the alginate gel fiber. An antibody production method is thereby additionally provided.

A cell culture tool configured to be produced in a simple facility to obtain a cell mass having a desired shape. The cell culture tool includes a cell culture base layer that contains a cell culture base. The cell culture base layer has a cell adhesive area to which a cell is adherable and a cell adhesion inhibitory area where cell adhesion is inhibited. The cell adhesion inhibitory area includes a modified product of the cell culture base.

Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), for example.

Bioreactors for production and recovery of medium chain carboxylates from organic biomass are disclosed. Methods for improved production and recovery of medium chain carboxylates from organic biomass are also disclosed. The bioreactors can be used as a chain-elongation bioreactor, and a method of use thereof results in improved production and recovery of medium chain carboxylates from organic biomass. The bioreactor includes a shell defined by one or more walls and a length, and a plurality of porous hollow fiber membranes placed inside the reactor for continuous liquid-liquid extraction, as well as granular activated carbon (GAC) as biocarriers. The plurality of hollow fiber membranes is mounted such that a percentage of the length of the shell remains unoccupied by the plurality of porous hollow fiber membranes.

Embodiments described herein generally relate to methods and systems for configuring settings of a cell expansion system including a bioreactor. Through a user interface, a user may configure display settings, system settings, and settings associated with protocols for loading, growing and/or harvesting cells. In configuring settings for protocols and associated processes, a diagram view or window of the cell expansion system is displayed in embodiments. The diagram view displays the process settings as graphical user interface elements. Settings available for configuration are enabled for selection in the diagram view. The diagram view allows the user to visualize the settings available for task configuration and to configure enabled settings. Configured settings are stored and capable of retrieval for subsequent execution or modification of the applicable protocol.

Embodiments described herein generally relate to methods and systems for customizing protocols for use with a cell expansion system. Through a user interface, a user may create a custom task for loading, growing and/or harvesting cells. A custom task may comprise one or more steps, in which a user may add or omit steps, as desired. Data may be entered for settings associated with a custom task, in which embodiments provide for such data to be entered each time the custom task is performed. In another embodiment, the settings for a custom task may be configured, in which such settings may be stored and retrieved upon selection of the custom task. Customization and configuration of a custom task may occur using a diagram view of the cell expansion system, in which process settings are associated with graphical user interface elements.

Proposed are a three-dimensional structuring method of cells and a three-dimensional structuring system of cells capable of efficiently bonding multiple cell clusters in a three-dimensional direction, pursuant to their growth, while ensuring safety. A plurality of fibers, in which one end of each of the fibers is held by a flat plate, are inserted together with the flat plate into a flow path through which a culture solution is supplied, a plurality of cell clusters are placed in the flow path upon causing the cell clusters to run with a liquid flow of the culture solution, and each of the cell clusters is cultured by being stacked on an outer surface of each of the fibers with the flat plate as a growth origin.

Embodiments described herein generally relate to methods and systems for configuring settings of a cell expansion system including a bioreactor. Through a user interface, a user may configure display settings, system settings, and settings associated with protocols for loading, growing and/or harvesting cells. In configuring settings for protocols and associated processes, a diagram view or window of the cell expansion system is displayed in embodiments. The diagram view displays the process settings as graphical user interface elements. Settings available for configuration are enabled for selection in the diagram view. The diagram view allows the user to visualize the settings available for task configuration and to configure enabled settings. Configured settings are stored and capable of retrieval for subsequent execution or modification of the applicable protocol.

A cell fiber production system comprises: a first flow path through which a first fluid containing a cell flows; a second flow path for allowing a second fluid for preparing a hydrogel to converge around the first fluid in the first flow path; and an ejection port through which at least the first fluid and the second fluid are discharged together. The cell fiber production system is constructed that, at a start stage of production of a cell fiber, the first fluid is delivered after the second fluid reaches at least a convergence point between the first flow path and the second flow path.

A cell sensor and methods of using the cell sensor for differentiating types of cells. The cell sensor includes a sensing member having bioscaffolds comprising nanofibers/nanowires of titanate grown on a titanium sheet. The method includes preparing a cell sensor comprising a sensing member having bioscaffolds comprising nanofibers/nanowires of titanate grown on a titanium sheet; incubating the bioscaffolds with cells in an aqueous solution at an incubation temperature for a period of incubation time, wherein the cells in the aqueous solution include at least one of cancer cells, normal cells, stem cells, and neuron cells; and measuring electrical characteristics of the bioscaffolds to determine the types of the cells based on the measured electrical characteristics.