The present invention provides a cell cultivation device comprising: a polymer porous film; a cell cultivation part that accommodates the polymer porous film; a culture medium supply means that is positioned at an upper section of the cell cultivation part; and a culture medium recovery means that is positioned at a lower section of the cell cultivation part, wherein the polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, the holes in the surface layers A and B are in communication with the macrovoids, and the cell cultivation part comprises a side part and a bottom part that has one or more culture medium discharge ports.

A large circulating fluidized bed cell bioreactor and a method for culturing animal cells. The reactor comprises an agitator tank body (01), an agitator tank base (50), a water inlet silicone hose (06), a backflow silicone hose (08), a cell culture tank (10), a culture tank base (40), a backflow pipe (15), a water inlet pipe (12), an agitator (20), a reactor tray (30), and a tube support plate (60). By means of cooperation among the above components, defects and shortcomings of fluidized bed cell bioreactors in the prior art including proneness to microbial contamination, a low level of dissolved oxygen, and a low working volume, are effectively resolved, advantageous in the popularization and application of this technology.

Bioconversion processes are disclosed in which biocatalysts including microorganisms or isolated enzymes that are substantially irreversibly retained in the interior of an open, porous, highly hydrophilic polymer are cycled between at least two different fluid media for the bioconversion of one or more substrates to one or more bioproducts. The processes are particularly attractive for using gas phase or using liquid feedstocks containing the substrate.

Disclosed are processing systems and processes for carrying out enzymatic batchwise or continuous process for the production of fatty acid alkyl esters for use in the biofuels, food and detergent industries.

The present invention comprises methods and systems for manipulation of media and particles, whether inert materials or biomaterials, such as cells in suspension cell culture. The methods and systems comprise use of an apparatus comprising a rotating chamber wherein the actions of the combined forces of gravity, fluid flow force and centrifugal force form a fluidized bed within the rotating chamber.

A compressible biomass carrier for use in fluid treatment systems is disclosed. The biomass carrier includes an extruded circumferential mesh-like structure and elongated elements positioned in a space confined by the circumferential mesh-like structure and at least some of the elongated elements are joined to an inner portion of the mesh-like structure.

The present invention relates to an apparatus and a method for fermenting, separating, and refining a product, which is produced by cultivating a microorganism. The apparatus and the method for fermenting, separating, and refining, of the present invention, can separate and refine the product that is produced by microbial fermentation in a simple, continuous manner and with high efficiency.

A moving bed biofilm reactor system for growing microorganisms comprising a reservoir containing a fluid that contains nutrients conducive to growth of microorganisms and a plurality of inert biomass carriers having a surface area configured to support growth of microorganisms is provided. The microorganisms may be used to remove a pollutant from a fluid. Furthermore, the microorganisms may be harvested and used as a foodstuff fertilizer, biofuels, and bioplastics.

This disclosure describes a biochemical reactor with fixed feed conduit. The biochemical reactor may include a tank configured to house immobilized carriers and fluid. The biochemical reactor may include a circulation conduit at least partially disposed within the tank. The circulation conduit may include a circulation inlet opening and a circulation outlet opening. The biochemical reactor may include one or more vanes disposed proximate to the circulation outlet opening. The one or more vanes may be configured to cause the immobilized carrier and the fluid exiting the circulation outlet opening to enter into a helical pattern. The biochemical reactor may include a feed conduit having a feed outlet. The feed outlet may be disposed between a first end and the circulation inlet opening. The feed conduit outlet may be immovable with respect to the circulation inlet opening.

A bioreactor has a biofilm that receives a gas through a supporting membrane and another biofilm attached to an inert support. The first biofilm is aerated through the membrane and provides nitrification. The other biofilm has an anoxic or anaerobic zone and provides denitrification. A module useful in the bioreactor has cords potted in at least one potting head. Optionally, some or all of the cords have a gas transfer membrane. The module may provide inert supports, active gas transfer supports or a combination of both types of support. Multiple modules may be assembled together into a cassette, the cassette providing inert supports, active supports or a combination. The module or cassette may have an aerator for mixing or biofilm control.