A container system includes a bag comprised of one or more sheets of flexible polymeric material, the bag having an interior surface at least partially bounding a chamber, the chamber being adapted to hold a fluid. A flexible sparging sheet is secured to the interior surface of the bag so that a compartment is formed between the interior surface of the bag and the sparging sheet, at least a portion of the sparging sheet allowing gas to pass therethrough. A tubular port or tube is secured to the bag so that a passage bounded by the tubular port or tube communicates with the compartment. A mixing element is disposed within the chamber of the bag, the mixing element being spaced apart from the flexible sparging sheet.

A gassing unit for bubble-free introduction of process gas into a liquid in a reactor, wherein the gassing unit includes at least: first and second spaced apart gas receiving chambers, the two gas receiving chambers connected to one another via at least two two-dimensional, gas-conducting diffusion membranes including hollow fibers spaced apart from one another and at least partially fixed to one another; a gas supply receptacle on at least one gas receiving chamber; a shaft receptacle on at least one gas receiving chamber;
wherein the gassing unit for gassing the liquid in the reactor is supplied with process gas via the gas supply receptacle, is set into a rotational movement via the receptacle for the shaft and forms a convection flow within the reactor via rotational movement of the gassing unit in the liquid. Further included is a method for gassing a process liquid, a gas-liquid reactor including a gassing unit, and the use of a gassing unit for supplying biological cultures with process gases.

Systems and methods for cyanobacteria indoor air biofiltration to reduce air handling energy consumption are disclosed. A system may include: a heating, ventilation, and air conditioning (HVAC) system comprising an air valve that controls a flow of conditioned, untreated air from an enclosed area; and a biofiltration device comprising: a controller that interfaces with the air valve and controls the air valve to open or close; an air inlet that receives the untreated air from the air valve when the air valve is in an open position; a bioreactor comprising a bio-organism; an air bubble mixing system that receives the untreated air and diffuses the untreated air into the bioreactor, wherein the bio-organism is configured to consume CO.sub.2 via photosynthesis and to release treated air; and an air outlet that receives the treated air from the bioreactor and expels the treated air to enclosed area.

A closure 1; 1 for a bioreactor 200; 200; 200, said closure being configured for being connectable to a container 100; 100; 100 such that the closure 1; 1 and the container 100; 100; 100 together constitutes the bioreactor 200; 200; 200, wherein the closure 1; 1 comprises an impeller device 11 comprising an impeller shaft 13 and impeller blades 15 mounted to the impeller shaft 13, wherein said impeller device 11 is mounted to the closure 1; 1 such that the impeller shaft 13 and the impeller blades 15 protrude into the container 100; 100; 100 when the closure 1; 1 is connected to the container 100; 100; 100 and a gas delivery device 21 which is arranged in the closure 1; 1 such that it passes from a gas inlet 23 at an outside surface 3b of the closure 1; 1 to an inside surface 3a of the closure 1; 1 and protrudes from the inside surface 3a such that a gas outlet 25 of the gas delivery device is provided inside the container 100; 100; 100 when the closure 1; 1 is connected to the container 100; 100; 100.

A biopharmaceutical liquid reservoir, includes a bag forming an inner storage space for storing a biopharmaceutical liquid, a mechanical member located at a wall of the bag, having: a stationary set of parts that is stationary with respect to the wall of the bag, a rotating set of parts rotating about an axis of rotation with respect to the stationary set, a bearing located between the two sets and, inside the bag, a communication passage: separating the bearing from the inner storage space, including one or more changes of direction, being formed by a portion of the parts of the rotating set located opposite a portion of the parts of the stationary set.

An impeller assembly for a bioprocessing system includes a hub, a plurality of blades disposed along a circumferential direction of the hub and spaced apart from each other, and at least one vent hole in the impeller assembly providing a pathway for gas to travel from an area beneath the impeller assembly to an area above the impeller assembly.

An extraction apparatus for a fermentation medium comprises an extraction chamber configured to receive a fermentation medium and comprising a lower portion and an upper portion above the lower portion, as well as a feed device configured to feed at least one gas and one liquid into the lower portion of the extraction chamber, as well as a discharge opening arranged in the upper portion of the extraction chamber and configured to drain a washout liquid, loaded with microorganisms, from the extraction chamber.

A method for the preparation of a mycelium colonized substrate includes a step of incubating a mycelium-inoculated substrate to grow the mycelium. The mycelium-inoculated substrate comprises a synthetic granulometry regulator. An intermediate product in the preparation of a mycelium colonized substrate includes mycelium colonized substrate and a synthetic granulometry regulator. A solid-state mycelium bioreactor is adapted for performing the method.

Miniature stirred tank bioreactor and system; the bioreactor configured for single use culturing, providing unobstructed culturing volume with sensors, probes and ports using minimally invasive placement in-wall or across the wall methods; the bioreactor further capable of containing scaffolds and features for culturing cells and tissue, including structures providing surfaces for cell growth, and screens for retaining microparticles during media exchange.

The invention relates to a method for recovering a biocatalytically produced organic substance from a reaction mixture, comprisingproviding a reaction mixture, wherein the organic substance is produced using a biocatalyst, which reaction mixture comprises a substrate for the biocatalyst in a continuous aqueous phase, and wherein further a product recovery phase is present into which the organic substance migrates or onto which the organic substance absorbs or adsorbs; andseparating the product recovery phase comprising the produced substance from the aqueous phase and the biocatalyst. The invention further relates to a bioreactor system for biocatalytically producing a substance, comprising an apparatus, said apparatus comprising a reaction compartment (11) situated in a lower part of the apparatus and a separator compartment (9).