Fixed bed bioreactor with natural convection and forced draught to obtain bioactive substances by solid-state fermentation (SEF) using fungi macromycetes. This bioreactor may be from turn drum with pendulum motion and natural and forced convection to a tray bioreactor with natural convection. They are used in the production of bioactive substances as crude extracts of lignocellulosic enzymes and fungal polysaccharides obtained by using mixtures of lignocellulosic materials as substrates and macromycetes fungi as inoculum, controlling pH, humidity and particle size, inoculation rate and environmental conditions during fermentation such as temperature, relative humidity and carbon dioxide and oxygen concentration.

A bioreactor system includes a reactor tank, a liquid input line in fluid communication with the reactor tank to provide a liquid medium to an interior of the reactor tank, a gas input line in fluid communication with the reactor tank to provide a gas to the interior of the reactor tank, and an agitator positioned in the reactor tank. The bioreactor system includes a sight glass disposed on a wall of the reactor tank. The sight glass is positioned at a first height from a bottom of the reactor tank. The first height lower is than a second height indicative of a maximum fill height for the liquid medium within the reactor tank. The sight glass is adapted to interact with light-based measurement technologies.

A container for fermenting bio-degradable substances, the container comprising: a base and a wall, wherein an interior of the container is enclosed by the base and the wall; a rotatable agitator that protrudes into the interior and is supported in an upper bearing and a lower bearing in an installed position of the rotatable agitator in the container, wherein the rotatable agitator includes a shaft that is drivable by a drive, wherein at least one stirring paddle is arranged at the shaft and configured to co-rotate with the shaft so that substances arranged in the container are stirrable, wherein the lower bearing is arranged at the at least one base of the container and a bearing device located at a lower end of the shaft is supported in the lower bearing in the installed position of the rotatable agitator.

A pneumatic bioreactor includes a vessel containing a fluid to be mixed and at least one mixing device driven by gas pressure. A first embodiment includes a floating impeller that rises and falls in the fluid as gas bubbles carry it upward to the surface where the gas is then vented, permitting the impeller to sink in the fluid. The floating impeller may be tethered to a second impeller with a flexible member and pulley. The mixing speed is controlled with electromagnets in the vessel acting upon magnetic material in the impeller or its guides. In another embodiment, floating pistons mix the fluid, pushing it through a mixing plate with one or more apertures. In a third embodiment, the mixing device is a rotating drum with bubble-catching blades and rotating mixing plates with apertures. The top of the vessel for these mixers may include a closed top and sterile filters.

A biogas plant contains a fermenter vessel, in which an assembly support is mounted, preferably vertically oriented. An assembly, in particular a submersible motor driven stirrer, is retained by a height adjustment device so as to be vertically adjustable on the assembly support. Accordingly, the height adjustment device is formed by a belt drive of which a traction device is guided around at least two deflecting elements spaced apart from one another in the longitudinal direction of the assembly support. At least one of the deflecting elements can be driven in rotation by a driving device and the assembly is coupled to the traction device in such a way that when the deflecting element is rotationally actuated in a first direction the assembly is raised and when the deflecting element is rotationally actuated in a second, opposite direction, the assembly is lowered.

A magnet-driven bistable dynamic bioreactor, its manufacturing method, and application thereof are provided. The bioreactor has a magnet-driven bistable actuator, a magnetic field generation module, and a connected temperature control module. The magnet-driven bistable actuator includes a magnetic bistable arch membrane. The magnetic field generation module provides a direction-alternating magnetic field, which drives the magnetic bistable arch membrane to switch states between different configurations. This process applies adjustable frequency biaxial bi-directional loading on cells that cultured on membrane, simulating real tissue loading. The temperature control module regulates the temperature of the magnet-driven bistable actuator. The bioreactor enables adjustable frequency biaxial bi-directional loading on cells cultured on the magnetic bistable arch membrane by utilizing an alternating magnetic field to switch its configuration. This process effectively simulates the loading environment experienced by cells in real tissues.

The present set of embodiments relate to a bioproduction system, method, and apparatus for creating a scalable bioreactor system. Specifically, the present set of embodiments enable the determination of bioreaction performance characteristics of a commercial scale by matching operational parameters between a small test scale bioreaction to that of a commercial scale bioreaction. The system and methods do not rely on simply making bioreactor apparatuses across scales the same dimensionally which would not account for differences in fluid dynamic properties between very small to very large volumes, but requires tuning of a variety of systems (mixing assembly, sparger system, and headspace airflow system) in conjunction with one another to achieve predictive outcomes.

A method of operating a fermentation device has an elongated container and multiple stirrer devices. The method includes introducing substrate containing organic material via the at least one introduction opening, moving and mixing the substrate in the vessel via the rotationally driven stirrer devices, removing treated material via the at least one discharge opening, and withdrawing biogas via the at least one draw opening. The stirrer devices are driven by a drive device in at least two groups. Each group includes at least one stirrer device. The stirrer devices of a first group are driven in at least one first time interval. Stirrer devices of a second group are stationary in the first time interval.

A fluid mixing system includes a support housing having an interior surface bounding a chamber. A flexible bag is disposed within the chamber of the support housing, the flexible bag having an interior surface bounding a compartment. An impeller is disposed within the chamber of the flexible bag. A drive shaft is coupled with the impeller such that rotation of the drive shaft facilitates rotation of the impeller. A drive motor assembly is coupled with the draft shaft and is adapted to rotate the drive shaft. An adjustable arm assembly is coupled with the drive motor assembly and is adapted to move the drive motor assembly which in turn moves the position of the drive shaft and impeller. An electrical controller can control movement of the adjustable arm.

A fermentation device includes an elongated container having an inlet opening at a first end face of the container, a discharge opening at a second end face of the container opposite the first, and an outlet opening for biogas. The device has a plurality of stirring devices and a drive device therefor. Each stirring device has at least one stirring shaft arranged transversely to a longitudinal axis of the container and can be rotated about a central axis of the stirring shaft via a drive device, and a stirring blade fixed to the shaft and protruding outwards. A method for operating the fermentation device includes introducing a substrate which contains organic material, moving and mixing the substrate in the container via the stirring devices, discharging treated material, and removing biogas via the outlet opening. Solely fresh substrate, untreated by the fermentation device, is supplied via the inlet opening.