A vessel having a mixer that ensures a homogeneous cell distribution in dispensed quantities. The vessel has a mixer therein for stirring contents of the vessel and an orifice in a lower wall to which a cell dispenser is attached. The cell dispenser dispenses quantities of suspended cells having a homogeneous cell distribution. The vessel may be a closed system with no option or instructions for removing a cap or other access port, whereby after manufacturing the vessel is shipped to a customer and the interior remains closed to the exterior environment and fluids are transferred through tubes.

Bioreactor systems for culturing mixotrophic microorganisms in open cultures on a large scale are disclosed herein. Embodiments of the system comprise organic carbon delivery systems and submersible thrusters suspended on adjustable support structures.

A wood chip fermentation device includes: a chip fermenter 11 that is charged with wood chips serving as a heat source and that ferments the wood chips; a temperature sensor 11a that is provided inside the chip fermenter 11 and measures the temperature inside the chip fermenter; a thermal energy extracting pipe 12 that is disposed inside the chip fermenter and extracts fermentation heat from inside the chip fermenter; a pump section 13 that supplies a medium to or takes out a medium from the thermal energy extracting pipe 12; a stirring blade 14a that stirs wood chips 10a put in the chip fermenter 11; and a discharge conveyor 15 that is disposed at a lower portion of the chip fermenter 11 and discharges fermented material. The stirring blade 14a is disposed between the thermal energy extracting pipes 12 and a rotating surface thereof is in a state of being parallel to the thermal energy extracting pipes 12. A bottom surface of the chip fermenter 11 is formed in an arcuate shape such as to follow the rotating outer peripheral surface of the stirring blade 14a. A plurality of temperature sensors 11a are installed in a vertical direction inside each chip fermenter. Control of an on-off valve of the thermal energy extracting pipe 12 is performed based on temperature information from the temperature sensors 11a.

Embodiments of the present invention provide novel, low-cost fermentation systems and methods of their use. More specifically, the present invention provides biological reactor systems for fermenting a wide variety of, for example, bio level 1 microorganisms with very high cell densities. The reactor systems can be used to grow yeast, fungi and bacteria, as well as growth by-products thereof. In specific embodiments, the reactor systems are used to produce yeast-based compositions. In certain specific embodiments, the reactor systems can be used for the production of Starmerella bombicola yeast compositions.

Discloses is a continuous fermenter for sequential fermentation of hexose and pentose which includes (a) a hexose fermenter equipped with a saccharified solution supply unit containing hexose, pentose and lignin, a plurality of trays closing at least half of the diameter of the fermenter, impellers disposed on each of the trays, an impeller driving unit, a lignin discharge unit disposed at the bottom of the fermenter, a fermented solution discharge unit, and a temperature control jacket; and (b) a pentose fermenter equipped with a fermented solution supply unit for supplying the fermented solution discharged from the hexose fermenter, a plurality of trays closing at least half of the diameter of the fermenter, impellers disposed on each of the trays, an impeller driving unit, a lignin discharge unit disposed at the bottom of the fermenter, a fermented solution discharge unit, and a temperature control jacket.

An aerobic organic material processor includes a tank having an outer wall, a top, a bottom, a first end, a second end, and a horizontal axis. The tank is divided into compartments distributed along the horizontal axis of the tank between the first end of the tank and the second end of the tank. Each compartment is connected to other compartments by a fluid passageway toward the bottom of the tank. An agitator is provided for agitating the organic material within the tank. Fluid ports are positioned toward the top of the tank in communication with each of the compartments. Applying either a suction force or pressure to at least one of the fluid ports causes fluids to migrate from compartment to compartment via the fluid passageway, thereby aerating organic material within the tank.

An anaerobic digestion system for producing biogas from biomass, particularly biomass that is lignocellulosic, and includes two separate and interacting anaerobic reactor environments to solubilize lignocellulosic materials and make them available for conversion to biogas.

The present application provides a reactor with an inclined bottom gas-inlet for aerobic fermentation. A fermenter is provided with a circular inner tank, end covers and a jacket. An airtight fermentation space is formed in the fermenter by the inner tank, an upper end cover and a lower end cover. A feed opening and an exhaust outlet are arranged at an upper part of the fermenter, and a discharge opening is arranged at a lower part of the lower end cover of the fermenter. An energy-saving stirrer is mounted in the fermenter. A plurality of air chambers are arranged at an external wall at the bottom of the inner tank of the fermenter and are within the jacket. A plurality of aeration nozzles are defined on an inner side of each air chamber, and the aeration nozzles are disposed next to the inner tank.

A bioprocessing apparatus includes a flexible bag having an interior volume configured to contain a fluid, and an integral fluid conduit within the flexible bioprocessing bag. The integral fluid conduit includes a panel of material joined to an interior sidewall of the flexible bag so as to define a fluid channel between the interior sidewall of the flexible bag and the panel of material. The integral fluid conduit may include a bottom outlet opening, a top outlet opening, or both. The apparatus may further include a port in a top of the flexible bag, a port in the bottom of the flexible bag, or both, wherein the integral fluid conduit is fluidly connected to the ports.

An apparatus for hydrolysing a product, comprising a continuous conduit having an inlet for receiving the product and an outlet for discharging the product, the conduit being formed so as to follow a path that winds around a longitudinal axis as the conduit extends parallel to the longitudinal axis between the inlet and the outlet, and a heating system for heating at least part of the conduit. The conduit is rotatable about the longitudinal axis so as to transport product received in the inlet towards the outlet.