A biological production system includes a mixing vessel and an agitation device. The mixing vessel includes an outer housing, an internal mixing structure, and a reaction chamber. The mixing vessel is configured to provide low shear agitation to materials in the reaction chamber in response to force from the agitation device.

A yogurt maker device comprises an outer shell to house a yogurt vessel that receives milk and a culture container that receives yogurt culture. A processor within the outer shell controls a heating element to boil milk within the yogurt vessel using a temperature sensor as feedback and for maintaining a predefined boiling temperature for a predefined period of boiling time and a stirring device to continuously stir boiling milk within the yogurt vessel while boiling and also while cooling to a cooling predefined temperature until fermentation temperature is reached. The processor also controls a culture container base holding the culture container and having a motor to automatically pour the yogurt culture when the fermentation temperature is reached. Finally, a cooling mechanism automatically provides cooling to contents of the yogurt vessel once fermentation is complete.

The solution proposed by the present invention consists of the different shape of the bottom of a horizontal digester (1) having at the terminal part of its length (the end side), at the opposite end from the feed inlet side, a sediment separator (9) which is in the shape of a conic pyramid (91), a linear V (92), or a mixed form (96), which is emptied periodically, allowing the removal of the sediments deposited on the bottom. In the conic pyramidal sediment separator (91) the emptying occurs by gravity through a controlled valve (93), while in the V-shaped linear sediment separator (92) or the mixed-form sediment separator (96) the emptying is by means of a helical impeller (94) at the bottom. The sediment separator allows the use of an organic fraction of municipal solid waste coming from a less sophisticated selection process with a greater presence of residual inorganic waste.

The present invention relates to a process for production of fermentation products, including bioethanol by non-pressurised pre-treatment, enzymatic hydrolysis and fermentation of waste fractions containing mono- and/or polysaccharides, having a relatively high dry matter content. The process in its entirety, i.e. from non-pressurised pre-treatment over enzymatic hydrolysis and fermentation to sorting of fermentable and non-fermentable solids can be processed at a relatively high dry matter content in a single vessel or similar device using free fall mixing for the mechanical processing of the waste fraction.

A tempering device for tempering of biomass contained in a container, including at least one tempering mechanism, and at least one conveying device for conveying a mass flow of biomass in a main flow direction. The at least one conveying device includes at least one agitator, and wherein the at least one tempering mechanism is arranged in the main flow direction generated by the at least one conveying device. The at least one tempering mechanism is tubular with an inner tube and with an outer tube arranged coaxially to the inner tube. The inner tube and the outer tube form an intermediate space through which a tempering medium may flow. The tempering mechanism is oriented relative to the conveying device such that, in operation of the heating device, at least 40% of the delivery volume of the mass flow generated by the conveying device flows through the inner tube.

A process for optimizing the operation of a plug-flow fermenter for the anaerobic fermentation of organic wastes, wherein the plug-flow fermenter comprises a horizontally oriented fermenter tank and a stirrer, which stirrer comprises a stirrer shaft which traverses the interior of the fermenter tank in an axial manner and multiple paddles which are arranged on the stirrer shaft and protrude radially and also a drive, and the fermentation material is moved in the fermenter tank by means of the stirrer.

Height adjusting device (1) for adjusting the height of an agitating device (2) in a digester (101) of a biogas plant (100), biogas plant (100) comprising a digester (101), a support unit (10), a displacement member (6), an agitating device (2), and a height adjusting device, and method for operating an agitating device (2). The height adjusting device comprises a housing unit (4), a drum device (5) rotatably accommodated on the housing unit (4), and a displacement member (6). The displacement member (6) is in functional connection with the drum device (5) and the agitating device (2) and the displacement member (6), depending on the direction of rotation of the drum device (5), can be wound onto the drum device (5) or unwound from the drum device (5) in sections, so that the agitating device (2) can be displaced upwardly or downwardly. Furthermore a fixing device (7) is provided which is suitable and configured to fix the drum device (5) at least temporarily to prevent the displacement member (6) from unwinding if the displacement member (6) is not retained tautly between the drum device (5) and the agitating device (2).

A method and a device for adherent culture of microalgae in a thin liquid layer by mobile mechanical stirring, comprising: a subsystem for adherent culture of microalgae in thin liquid layer, for adherent culture of microalgae in a culture solution, comprising a microalgae liquid pool and a culture plate used as a base plate for microalgae seed inoculation, adherent culture, microalgae harvesting, the microalgae liquid pool containing culture solution having a water layer with a depth in a range of 1{tilde over ()}5 cm, forming a thin liquid layer; and a subsystem for mechanical stirring, comprising a mechanical stirring assembly, a moving assembly, a track member, a power drive assembly, a control assembly, the moving assembly moving on the track member to drive the mechanical stirring assembly to stir and suspend the microalgae, the microalgae standing and gradually settling to the bottom after stirring, realizing adherent culture in suspension and settlement cycle.

The present invention relates to a method of expanding stem cells cultured as cell aggregates in a suspension culture changing culture medium and a method of medium exchange for the same cells characterized in the use of a rotating mesh such as a spinfilter device. The present invention further relates to a use of a rotating mesh for medium exchange in a suspension culture of stem cells.

Materials and methods to control a nutrient feed in a cell culture process is provided. A sample is received from a bioreactor comprising a cell culture. A viable cell density and a residual nutrient measurement are determined from the received sample. A daily nutrient feeding target is calculated based on the viable cell density and the residual nutrient measurement. The nutrient is fed to the bioreactor according to the calculated daily nutrient feeding target.