One aspect of the invention provides a network platform that includes a fluidic network comprising one or more pumps, and one or more valves, and a plurality of fluidic modules interconnected by the fluidic network of the one or more pumps and the one or more valves to allow controlled transfer of suspended cells, other substances, and fluids from one fluidic module to another, or self-circulation.

Disclosed is a rotating culture vessel based on a rotating culture technology using an RWV, by which cell seeding, liquid medium exchange, quality control and so on can be automated and degassing can be conducted simultaneously with liquid medium exchange without disturbing the cells under culture. Also disclosed is an automatic cell culture apparatus using the same. A rotating culture vessel, which contains cells and a liquid culture medium, to be attached to a horizontal rotating shaft of a rotating culture device to three-dimensionally culture the cells, wherein one or more inlets/outlets for supplying cells and a liquid culture medium at the early stage and then taking out the cultured cells, are formed at appropriate position of a flat cylindrical culture container; at least one pair of a supply port and a discharge port for liquid medium exchange is provided on the outer circumferential cylindrical face of the culture container.

Disclosed is a rotating culture vessel based on a rotating culture technology using an RWV, by which cell seeding, liquid medium exchange, quality control and so on can be automated and degassing can be conducted simultaneously with liquid medium exchange without disturbing the cells under culture. Also disclosed is an automatic cell culture apparatus using the same. A rotating culture vessel, which contains cells and a liquid culture medium, to be attached to a horizontal rotating shaft of a rotating culture device to three-dimensionally culture the cells, wherein one or more inlets/outlets for supplying cells and a liquid culture medium at the early stage and then taking out the cultured cells, are formed at appropriate position of a flat cylindrical culture container; at least one pair of a supply port and a discharge port for liquid medium exchange is provided on the outer circumferential cylindrical face of the culture container.

A recipient for cell cultivation having an inner compartment adapted for cell growth, in one embodiment, an outer tubular wall extends in a longitudinal direction and delimits an outer boundary of the inner compartment in a radial direction. First and second ends delimit the inner compartment at the first respectively the second outer end of the outer tubular wall. A fixed packing in the inner compartment comprises a packing, such as a fiber matrix. Additional embodiments and related methods are also disclosed.

The present invention relates to methods for production of undifferentiated or differentiated embryonic stem cell aggregate suspension cultures from undifferentiated or differentiated embryonic stem cell single cell suspensions and methods of differentiation thereof.

A rotating bioreactor. A bioreactor base has a cover support section positioned within the base. Air holes extend through the base and connect to the cover support section. An air permeable membrane is positioned on top of the cover support section and covers the air holes. A base cover has a chamber tube extending through the base cover. The base cover is inserted onto the base and is supported by the cover support section. A sealing mechanism is positioned between the base cover and membrane. A septum is attached to the base cover. A cell containment chamber is formed within the rotating bioreactor and is bordered by the membrane, the sealing mechanism, the chamber tube and the septum. The cell containment chamber is air permeable but water and fluid impermeable.

The present invention relates to methods for production of undifferentiated or differentiated embryonic stem cell aggregate suspension cultures from undifferentiated or differentiated embryonic stem cell single cell suspensions and methods of differentiation thereof.

An in-site composting system for community kitchen waste is provided, which includes: a dump unit, configured to receive a kitchen waste; a power energy conversion unit, configured to convert a part of energy generated by a fitness equipment or a rocking handle into electrical energy, and output a remaining part of the generated energy directly as power, a biological conversion unit, configured to receive the kitchen waste fed by the dump unit and perform a composting and fermentation processing on the kitchen waste; an energy storage unit, configured to store the electrical energy generated by the power energy conversion unit and the solar panel; and a material outputting and returning unit, configured to be connected to the biological conversion unit, wherein the material outputting and returning unit is configured to output and return a material decomposed by the biological conversion unit.

The present invention provides a system and a method for enhancing mass transfer of a gas between a gaseous medium and a liquid medium in reactions involving such a mass transfer, and for effective mixing of the liquid medium.

The invention relates to a cell culture bottle for adherent cells (e.g. human mesenchymal stem cells), comprising: a vessel; an internal cylinder, which has an internal Archimedes screw; an internal central tube, through which liquid can flow; and at least one wall arranged around the central tube. This arrangement provides an enlarged inner surface for the growth of the cells and for the reliable mixing of the fluid. The cell culture bottle is formed as a single piece and can be simply and economically produced as a disposable device by means of additive manufacturing.