Systems and methods provide a self-contained sealed apparatus that captures, filters, compresses and stores methane gas produced by the decomposition of bio-degradable organic materials. The system includes a rotatable and sealable chamber with an intermittent drive unit that mixes moist bio-degradable material during an anaerobic reaction, and captures methane gas generated by anaerobic decomposition. A filter to remove impurities, a low-pressure storage tank, a compressor and a high-pressure storage tank are interconnected and controlled by a system that monitors system parameters, that may include gas flow rate, temperature, and gas volume, and controls system parameters, that may include drive unit activation, generator operation, and compressor operation.

Embodiments of a modular tubular bioreactor system for culturing an aqueous culture of microorganisms are described herein. The tubular bioreactor may comprise culture tubes configured in a vertically spaced and horizontally staggered arrangement to optimize the application of light to the culture in phototrophic and mixotrophic cultivation.

The invention relates to a device and process for converting organic waste to biogas with high methane content and improved organic conversion efficiency. Disclosed process consists of two stages and in the first stage, shredded organic waste is digested in primary digester in which biodegradable organic fractions present in waste gets converted to volatile fatty acids and alcohols dissolved in aqueous solution by hydrolytic and acidogenic microorganisms. Primary digester effluent pH, is adjusted to about 6.8-7.5 by addition of controlled alkali solution. Neutralized waste slurry is separated into liquid solution called as leachate and digested solid sludge. In the second stage, liquid leachate comprising volatile fatty acids are converted to biogas with methane content in the range 80-86% by methanogenic microbial culture in main digester under anaerobic conditions. This invention further describes optimized primary and main digester configurations with operating conditions to improve organic conversion efficiency in both the digesters.

A baffle (130) configured to be disposed in a cell culture vessel is provided. The cell culture vessel (100) may comprise a vessel body (101) comprising a bottom wall (110) and a plurality of side walls (107) that define a cell culture chamber, the bottom wall comprising a cell culture surface (111). The baffle (130) may be configured to be disposed parallel to the cell culture surface (111) within the vessel body, the baffle comprising a first baffle crossbar (133) extending across a length of the cell culture surface; a second baffle crossbar intersecting (135) the first baffle crossbar; and a third crossbar disposed (137) at an end of the first baffle crossbar.

A system for growing and reproducing microorganisms that includes a basin system that includes a number of basins, where each basin has a vertical meandering system which is formed by partitions and which can be illuminated, each basin is filled with a nutrient suspension, at least one outer wall of each basin is double-walled such that a cavity is formed, a temperature control medium for controlling the temperature of the nutrient suspension can flow through the cavity.

The invention relates to methods, devices and inserts for reactors for microbial and anaerobic digestion. In particular, the invention relates to reactors comprising inserts for biofilms, such as methane-producing biofilms, immobilized on a carrier matrix.

Embodiments of a modular tubular bioreactor system for culturing an aqueous culture of microorganisms are described herein. The tubular bioreactor may comprise culture tubes configured in a vertically spaced and horizontally staggered arrangement to optimize the application of light to the culture in phototrophic and mixotrophic cultivation.

A single-use cell culturing apparatus and a culturing bag are provided, each of which can improve efficiency of stirring a culture solution and further reduce a possibility of leakage of the culture solution. The single-use cell culturing apparatus of the present invention includes a housing that accommodates the culturing bag which is flexible and can seal the culture solution inside thereof. The housing has, on an inner surface thereof, a curved convex-shaped protrusion provided at a portion of a contact face at which the housing is in contact with the culturing bag. The culturing bag of the present invention is accommodated in the housing of the single-use cell culturing apparatus; is flexible; and can seal the culture solution inside thereof. The culturing bag includes a concave portion having a shape corresponding to the curved convex protrusion disposed on a part of the housing.

An agitator comprises: a bearing unit that holds a shaft unit of the agitator and slides rotatably with respect to the shaft unit; and a rotary blade unit that is fixed to the bearing unit and rotates together with the bearing unit. The bearing unit comprises a first member composed of a material and the rotary blade unit comprises a second member composed of a material different from that of the first member.

The present invention relates to bioreactor system and method thereof wherein support matrix (2) comprises at last one central shaft and plurality of peripheral shaft being radially surrounds central shaft. Arrays of discs (11) are mounted along the shaft by defining interspatial vicinities between two successive plates. Thus, discs mounted on peripheral shafts are rotated within the interspatial vicinity of discs of central shaft to ensures sufficient mixing and avoid stagnant fluidic zones which is created when discs are mounted closely apart from each other on shafts. Further, plurality of deflector vanes that are axially provided along the length of the central shaft to redirect substantially co-axial direction fluid flow into interior of culture vessel and more specifically towards the central axis. Thus, bioreactor system provides scalable and disposable bioreactor with efficient mixing and homogeneous conditions and thereby supports high density growth and maintenance of cells and other biological material.