A scalable horizontal single-use pressurizable modular multi-agitated portable fermentor for culturing microorganisms to high cell density with high oxygen mass transfer capability is provided. The fermentor is suitable for laboratory use, process development suites and large scale production facilities. The disposable sterile bag, constructed of thin polymer film, incorporates a single-use magnetically driven turbine impeller. The single-use bag is fully contained in a stainless steel bag retention vessel designed to permit the bag to be pressurized. Conventional fermentor control is used to facilitate oxygen mass transfer rates suitable for optimal microbial growth, metabolism, and recombinant protein product formation. Horizontal modules, each having an independent agitator, enables scaling-out while maintaining constant input power per unit volume. Increasing the bag retention vessel/bag diameter enables scaling-up to large batch sizes. Alternate impeller types are provided for high gas flow when needed to support high cell density cultures.

Separating apparatus, comprising a sedimentation settler and a collection vessel disposed underneath and being in fluid communication with the sedimentation settler, the collection vessel forming a receiving chamber having an outlet at or adjacent to the chamber bottom and having an inlet opening, wherein the collection vessel is arranged such the flow direction of the fluid in the area underneath the sedimentation settler is substantially in line with the direction of the channels of the sedimentation settler.

The present invention relates to large-scale bioreactors having at least two impellers, large-scale bioreactor systems and methods for the large scale cultivation and propagation of mammalian cells using these bioreactors.

This disclosure describes an improved heat transfer system for use in reaction vessels used in chemical and biological processes. In one embodiment, a heat transfer baffle comprising two sub-assemblies adjoined to one another is provided.

The present disclosure relates to a microfluidic circuit comprising a drug inlet port; an outlet port; a drug inlet main channel fluidically connecting the drug inlet port and the outlet port, where said drug inlet main channel comprises (i) a plurality of serpentine mixers and (ii) a plurality of dead-end first microchamber sets; a negative inlet port; a negative inlet main channel fluidically connecting the negative inlet port and the drug inlet main channel; a plurality (n) of ladder channels, where each of the plurality (n) of the ladder channels is fluidically connected to both the drug inlet main channel and the negative inlet main channel; and an outlet channel fluidically connected to the drug inlet main channel between the drug inlet port and the outlet port. Also disclosed is a microfluidic device comprising a microfluidic circuit of the present disclosure and a method for performing an assay.

A sampling system includes a graduated sampling chamber configured for fluid connection to a sample source, a pump device configured for fluid connection with the sampling chamber, and a sterile air filter intermediate the pump device and the sampling chamber, wherein the pump device is selectively actuatable to draw a volume of fluid from the sample source into the sampling chamber.

The present invention relates to a reservoir element (200) for a tank 1 of a bio-pharma process line, the reservoir element (200) including a body element (210), and at least one shell element (220, 222). The body element (210) has a first end (212) and a second end (214), being opposite the first end, each of the first and second ends including an orifice, and wherein the body element tapers from the first end to the second end, wherein an inner volume is defined between the first and second ends. The body element and/or the at least one shell element include at least one groove (216, 217), wherein the at least one shell element (220, 222) is fixedly attached to the body element (210) so that the body element and the at least one shell element sandwich the groove in between forming at least one channel, the at least one channel being adapted for guiding at least one biochemical medium and/or an operating medium.

A generally cylindrical collapsible mixing vessel, comprising: a) a flexible plastic film side wall, a top wall and a bottom wall, wherein both the top wall and the bottom wall are attached to the side wall and the side, top and bottom walls define an inner volume of the vessel; b) an agitator located in the inner volume and; c) at least one vortex breaker made from flexible plastic film, located in the inner volume and attached to the bottom wall and/or the side wall via string elements, wherein said vortex breaker extends along the side wall and inwards in a generally radial direction.

The invention relates to a method for a photochemical process, such as a photocatalytic and/or photosynthetic process, in particular for the culture and production or the hydroculture of microorganisms. A reaction medium (6) is conducted in a meandering manner in a reactor element (2) which is made of at least two upright and connected pipes (3) or chambers (13). Multiple reactor elements (2) are serially connected into a bio solar reactor (1), and a reaction medium (6) flow which is stress-free for the microorganisms is generated in the bio solar reactor (1) using hydrostatic pressure and level compensation. Inlet and outlet openings (4, 5) are arranged on the lower face (8) of each individual reactor element (2) on each of the outermost pipes (3) or chambers (13). The reaction medium (6) flows around all of the connections in the lower region, in particular the inlet opening (4), the outlet opening (5), and the introduction inlet (17). The invention also relates to a device for carrying out the method and to a bio solar reactor.

The subject matter herein provides a biotic method for recovering one or more hydrocarbons from a feedstock that includes one or more of oil shale, bituminous tar sand, coal and cellulous at atmospheric temperature and pressure. The method comprises loading the feedstock into a container, treating the feedstock in the container with a biomedium of micro-organisms, and forming an essentially liquid mixture from the feedstock and the biomass by rotary tumbling the feedstock and the biomedium in the container. The essentially liquid mixture is then separated into the one or more hydrocarbons by centrifuging.