A culture device by which microalgae can be satisfactorily cultured using a simple structure while suppressing an increase in energy consumption, and a culture method. A main body of this culture device comprises an accommodation part which accommodates contents and to which gas is supplied. The inner wall faces of the accommodation part are joined together to form a joined part which extends along the gas supply direction. A guide part and a circulation part, which are adjacent to each other across the joined part, are each along the extending direction of the joined part and communicated with each other via a guide part inlet and a guide part outlet. A gas supply port enables gas supply toward the guide part.

The invention proposes a high-pressure environment biological enrichment and spray-type solid isolation and cultivation device, including a spray-type isolation and cultivation solid unit, and a biological enrichment unit. In the case of constructing a high-pressure, low-temperature environment consistent with the marine environment, the biological enrichment unit is used to realize enrichment and multi-stage purification process of marine microorganisms, obtain a biological enrichment fluid and inject the biological enrichment fluid into the spray-type isolation and cultivation solid unit; and the spray-type isolation and cultivation solid unit is use to convert the biological enrichment fluid into a state of microbeads, so that the biological enrichment fluid can be isolated and cultivated in a dispersed state.

A method for the preparation of a mycelium colonized substrate includes a step of incubating a mycelium-inoculated substrate to grow the mycelium. The mycelium-inoculated substrate comprises a synthetic granulometry regulator. An intermediate product in the preparation of a mycelium colonized substrate includes mycelium colonized substrate and a synthetic granulometry regulator. A solid-state mycelium bioreactor is adapted for performing the method.

The present invention provides a system for culturing a microorganism, the system including: a culture tank; a recycled gas adjusting unit; a first gas circulation line having one end thereof connected to an upper part of the culture tank and another end thereof connected to the recycled gas adjusting unit; and a second gas circulation line having one end thereof connected to the recycled gas adjusting unit and another end thereof connected so as to supply a recycled gas into the culture tank, wherein the recycled gas adjusting unit mixes, in at least a portion of an off-gas discharged from the culture tank, one or a plurality of gas components selected from the group consisting of a combustible gas, a combustion-supporting gas, and an incombustible gas so that a concentration of the combustion-supporting gas in the recycled gas is in a prescribed range.

The invention relates to a method for recovering a biocatalytically produced organic substance from a reaction mixture, comprisingproviding a reaction mixture, wherein the organic substance is produced using a biocatalyst, which reaction mixture comprises a substrate for the biocatalyst in a continuous aqueous phase, and wherein further a product recovery phase is present into which the organic substance migrates or onto which the organic substance absorbs or adsorbs; andseparating the product recovery phase comprising the produced substance from the aqueous phase and the biocatalyst. The invention further relates to a bioreactor system for biocatalytically producing a substance, comprising an apparatus, said apparatus comprising a reaction compartment (11) situated in a lower part of the apparatus and a separator compartment (9).

Bioreactors including bioreactors having features for improved performance, sensing and ease of use. Many embodiments provide bioreactors having improved dip-tubes, magnetically coupled agitators, condensers and sensing ability. Particular embodiments provide a bioreactor including a vessel having an inner volume for liquid contents and a head plate (HP) for coupling a plurality of components to the bioreactor where the HP is coupled to a top portion of the vessel and includes a plurality of ports. A diametric magnetic (DM) drive assembly (DMDA) and agitation shaft (AS) including at least one impeller are rotatably coupled to the HP. The DMDA and AS are rotated by non-vertical magnetic forces from a rotating DM positioned above the HP. A dip tube assembly (DTA) having a plurality of inner fluidic channels is positioned through a HP port such that a DTA end extends into the vessel for delivery liquids and sparging gasses.

The present invention is a manufacturing method for producing fermentation products using a fermentation vessel, including steps of: preparing a fermentation vessel and a sensor, introducing liquid into the fermentation vessel, and operating the fermentation vessel in which properties of liquid in the fermentation vessel are measured to adjust operating conditions; wherein the sensor device has a sensor for measuring liquid properties and a sensor cover body; a bottom permeable portion for passing liquid and crystals in the liquid is disposed on the bottom surface of the cover body, and a top permeable portion for passing liquid and crystals in the liquid is disposed on the top surface of the cover body; micropores are respectively formed in the bottom permeable portion and the top permeable portion; and micropores disposed in the top permeable portion are the same or larger than micropores disposed in the top permeable portion.

Provide are a cell culture method which includes using a culture vessel which contains a cell suspension containing cells and discharging a gas including 30% by volume or more of oxygen into the cell suspension from a sparger disposed in the culture vessel to culture the cells, in which an average hole diameter in a gas discharge portion in the sparger is 1 m or more and 300 m or less, and a surface area A (m.sup.2) in the gas discharge portion in the sparger, a volume X (m.sup.3) of the cell suspension in the culture vessel, and a flow rate Q (m.sup.3/min) of the gas that is discharged from the sparger satisfy a specific relationship, a product producing method including the cell culture method, and a cell culture device that enables the cell culture method.

The invention provides a method and system for the enzymatic transesterification of fatty acids or fatty acid derived products to produce lubricant esters. The process involves a reaction mixture containing a fatty acid or fatty acid-derived substrate, an alcohol and a lipolytic enzyme catalyst. A reactive column with a porous frit is employed through which air is bubbled into a mixture of fatty acid derivatives, alcohol and an immobilized lipase catalyst. The air bubbles promote mixing and the subsequent stripping of volatile by-products, thereby increasing ester formation. This process can be carried out at ambient temperature, without the need for external heating or microwave irradiation. The method achieves greater that 99 mol % conversion to ester products at lower energy when compared to conventional thermochemical transesterification.

A culture device includes a composition ratio control mechanism that measures a composition ratio of a mixed gas in a space above a liquid surface of a culture solution in a culture tank and supplies an adjustment gas into the space in accordance with the measured composition ratio of the mixed gas to maintain the composition ratio of the mixed gas in the space at a target value outside a preset explosive range, and a dissolution rate control mechanism that measures, during the culture, a combustible gas dissolution rate, which is a proportion of a combustible gas dissolved in the culture solution, to the combustible gas contained in a raw material gas supplied to the culture tank through a gas circulation path or a raw material gas supply unit, and maintains the measured combustible gas dissolution rate at or above a preset target value.