A system for light stimulation of a biological sample includes a light source adapted to emit an input light beam, and a closed chamber to receive at least one container in which the biological sample is placed. The system includes an optical device, and an optical guide connected on one side to the light source so as to receive the input light beam and on the other side to the optical device, the optical guide being arranged to guide the input light beam emitted by the light source as far as the optical device and to emit an output light beam. The optical device is configured to generate, from the output light beam, a parallel stimulating light beam, with a predetermined constant section.

Provided are medium culturing and observing device and gas circuit control method thereof. Medium culturing and observing device includes housing body, culture box body, culture turntable, heating component, rotation module, microscopic imaging module, moving module, and gas circuit system. By providing culture turntable and moving module, culture dish is driven to rotate to observation window position by rotating turntable, and imaging is performed through microscopic imaging module. Since position of culture dish may not be perfectly aligned with center of observation window, moving module can drive microscopic imaging module to move, thereby aligning microscopic imaging module with culture dish. Therefore, culture turntable only needs to rotate once to transfer culture dish that needs to be observed to observation window, thus reducing rotation frequency of culture turntable, reducing vibrations affecting medium.

Systems and methods are disclosed for enabling optical bioprocesses in cell manufacturing. A cell manufacturing platform comprises a cell culture cassette supporting a cell culture; an optical engine configured to: capture images of the cell culture; and optically remove cells from the cell culture; and wherein the cell culture cassette remains stationary and the optical engine moves relative to the cell culture cassette to capture the images and to remove cells.