The invention relates to a method for processing biological substrate, comprising the steps of: a) introducing the biological substrate into an apparatus for dry fermentation, b) carrying out dry fermentation to recover methane, forming fermentation residue, c) transferring the fermentation residue obtained in step b) to an apparatus for solid-liquid separation, d) carrying out solid-liquid separation of the fermentation residue, forming a fermentation residue solid phase and a fermentation residue raw liquid phase, e) transferring the fermentation residue raw liquid phase obtained in step d) to an apparatus for separating solids, f) carrying out separation of solids from the fermentation residue raw liquid phase, recovering a fermentation residue liquid phase, g) introducing the fermentation residue liquid phase obtained in step f) into an apparatus for wet fermentation, h) carrying out wet fermentation to recover methane, forming liquid fermentation residue, i) returning at least some of the liquid fermentation residue formed in step h) as inoculum to the apparatus for dry fermentation.

Aspects of seaweed bioreactor apparatus, methods, and systems are described. One aspect is a method comprising: scaling a seaweed biomass and a culture medium in a light-transmitting vessel located in a light-controlling enclosure; and sampling or harvesting the seaweed biomass without unsealing the light-transmitting vessel by: causing, with a controller, a light source to output an artificial light into the light-controlling enclosure that illuminates the seaweed biomass; outputting, with a sensor in communication with the controller, sensory data associated with the culture medium; modifying, with the controller, at least one of the artificial light or the culture medium responsive to the sensory data; intermittently mixing and cutting, with the controller, the seaweed biomass in the light-transmitting vessel; and removing a portion of the seaweed biomass from the light-transmitting vessel and the light-controlling enclosure. Related seaweed bioreactor apparatus, methods, and systems also are described.

System and method for providing biocompatible, nutrient filled media to the Human Cells, Tissues, and cellular and tissue-based Products (HCT/P) while removing wastes. The present teachings provide for sensing the characteristics of the media, and modifying the characteristics when necessary. The present teachings can also provide components that can provide fluid pumping integrated with fluid gas exchange, and sensing of fluid characteristics at consistent times during the fluid flow cycle. System and method control multiple bioreactors from a centralized media reservoir, while fluidically isolating the bioreactors from cross-contamination.

Described are systems, methods, and devices relating to normothermic extracorporeal support of an organ, tissue, or bioengineered graft comprising cross-circulation (XC) perfusion for prolonged periods (days to weeks) via an XC perfusion circuit in connection with an extracorporeal host (e.g., animal, patient, organ transplant recipient) are disclosed. The XC perfusion circuit comprises auto-regulation of blood flow based on the trans-organ blood pressure difference between arterial and venous pressure. Recipient support enabled 36 h of normothermic perfusion that maintained healthy lungs with no significant changes in physiologic parameters and allowed for the recovery of injured lungs. Extended support enabled multiscale therapeutic interventions in all extracorporeal lungs. Lungs exceeded transplantation criteria.

System and method for providing biocompatible, nutrient filled media to the Human Cells, Tissues, and cellular and tissue-based Products (HCT/P) while removing wastes. The present teachings provide for sensing the characteristics of the media, and modifying the characteristics when necessary. The present teachings can also provide components that can provide fluid pumping integrated with fluid gas exchange, and sensing of fluid characteristics at consistent times during the fluid flow cycle. System and method control multiple bioreactors from a centralized media reservoir, while fluidically isolating the bioreactors from cross-contamination.

The present application provides an culture vessel active-oscillation slide mechanism and a shaker incubator, which can improve the overall stiffness and stability of the slide mechanism to avoid the creation of lateral bending moment while ensuring the culture vessel in the shaker incubator to fully extend out, and can also simplify the assembly complexity of a stroke amplification mechanism and reduce the number of parts. The culture vessel active-oscillation slide mechanism comprises a slide power transmission device, a slide stroke amplification mechanism and a slide mounting plate. The slide power transmission device can convert a rotational motion of an output shaft of a slide drive motor into a linear displacement in a direction of width. The slide stroke amplification mechanism amplifies the stroke of the linear displacement of the slide power transmission device in the direction of width. Rigid multi-stage rails are mounted on two sides of the slide mounting plate in a direction of length of the incubator body. In the multi-stage rails are configured to, by means of stages of rails partially sliding out relative to each other, support a culture vessel carrier mounting plate that fully extends out after the stroke is amplified.

The disclosure relates to bioreactors, for example for biological treatment and, more specifically to bioreactor insert apparatus including temperature control and substance delivery elements. The temperature control elements include conduits to circulate a thermal or working fluid and provide a means to locally maintain a biofilm support and biofilm thereon at a temperature promoting growth of the biofilm microorganisms. The conduits for temperature control can further serve as substance delivery elements, providing a means for selective transport and biofilm-localized delivery of growth-promoting substances in the circulating thermal fluid. The temperature control and substance delivery elements can increase the overall reaction rate of the bioreactor.

Described are systems, methods, and devices relating to normothermic extracorporeal support of an organ, tissue, or bioengineered graft comprising cross-circulation (XC) perfusion for prolonged periods (days to weeks) via an XC perfusion circuit in connection with an extracorporeal host (e.g., animal, patient, organ transplant recipient) are disclosed. The XC perfusion circuit comprises auto-regulation of blood flow based on the trans-organ blood pressure difference between arterial and venous pressure. Recipient support enabled 36 h of normothermic perfusion that maintained healthy lungs with no significant changes in physiologic parameters and allowed for the recovery of injured lungs. Extended support enabled multiscale therapeutic interventions in all extracorporeal lungs. Lungs exceeded transplantation criteria.

A method for the preparation of a mycelium colonized substrate includes a step of incubating a mycelium-inoculated substrate to grow the mycelium. The mycelium-inoculated substrate comprises a synthetic granulometry regulator. An intermediate product in the preparation of a mycelium colonized substrate includes mycelium colonized substrate and a synthetic granulometry regulator. A solid-state mycelium bioreactor is adapted for performing the method.

A bioreactor for the in vitro culture of reproductive tissues and the like in perifusion mode, including a first component and a second component, mutually connected, which define between them at least one culture chamber; the former component includes an inlet port for introducing a culture medium into the culture chamber, while the latter component includes an outlet port, to allow the culture medium out of the culture chamber. The bioreactor includes at least one porous medium, provided inside the culture chamber, able to mechanically support fragments of reproductive tissue, and networks, positioned above and below the fragments of reproductive tissue, with reference to the vertical or substantially vertical position of use of the bioreactor.