The present invention provides a biogas production system comprising: at least one digester for generating biogas and digestate, each digester comprising: a first end portion, a second end portion, and a peripheral side-wall there-between; one or more inlets, wherein at least one inlet is arranged to receive organic substrates; one or more outlets, wherein at least one outlet is arranged to release biogas; and a feed system comprising a pit arranged to receive and supply said organic substrates to the digester, said inlet positioned adjacent to a lower portion of said pit, the pit arranged such that a weight of the organic substrates within the pit bias the organic substrates into the digester.

A method for automated, artificial-intelligence-based IVF microtool control includes receiving a command at a controller associated with an artificial intelligence/machine learning system (AI/ML system) and an imaging system used at least in part to operate robotic components of in vitro fertilization (IVF) module. The method includes retrieving at least one IVF microtool assembly (MA) from a tool inventory location using a first robotic mechanism of the robotics system, based at least in part on the command. The method includes retrieving at least one IVF receptacle using a second robotic mechanism of the robotics system based at least in part on the command. The method includes placing the IVF receptacle on a stage within the IVF module based on the command. The method includes positioning the at least one MA in proximity to the IVF receptacle, using the first robotic mechanism, based at least in part on the command.

One or more methods for treating a tissue site comprises collecting a tissue sample that has keratinoytes and placing the tissue sample in a cartridge that has a well plate, positioning the cartridge within a base unit that has a tissue disaggregator, activating the base unit to rotate the well plate to align wells in the well plate with the tissue sample and the tissue disaggregator, operate the tissue disaggregator to separate the tissue sample to form a regenerative epidermal suspension, and provide the regenerative epidermal suspension to a tissue site to enhance healing of the tissue site.

A method for automated, artificial-intelligence-based IVF microtool control includes receiving a command at a controller associated with an artificial intelligence/machine learning system (AI/ML system) and an imaging system used at least in part to operate robotic components of in vitro fertilization (IVF) module. The method includes retrieving at least one IVF microtool assembly (MA) from a tool inventory location using a first robotic mechanism of the robotics system, based at least in part on the command. The method includes retrieving at least one IVF receptacle using a second robotic mechanism of the robotics system based at least in part on the command. The method includes placing the IVF receptacle on a stage within the IVF module based on the command. The method includes positioning the at least one MA in proximity to the IVF receptacle, using the first robotic mechanism, based at least in part on the command.

A system for converting a biomass into a biofuel including a biomass processing station arranged to receive the biomass from a biomass harvester, output the biomass to a hydrothermal liquefaction (HTL) converter, and receive a processed biomass from the HTL converter. The system includes a conduit arranged to transport the biomass from the biomass processing station to the HTL converter and transport the processed biomass from the HTL converter to the biomass processing station. The HTL converter includes a heat exchanger arranged to transfer thermal energy from a geothermal heat source to the biomass to convert the biomass into the processed biomass. The system also includes a controller arranged to monitor conditions of the biomass at locations along the conduit and adjust operations of components along the conduit to, thereby, adjust the conditions of the biomass at one or more locations along the conduit.

Embodiments of the present invention provide novel, low-cost fermentation systems and methods of their use. More specifically, the present invention provides biological reactor systems for fermenting a wide variety of, for example, bio level 1 microorganisms with very high cell densities. The reactor systems can be used to grow yeast, fungi and bacteria, as well as growth by-products thereof. In specific embodiments, the reactor systems are used to produce yeast-based compositions. In certain specific embodiments, the reactor systems can be used for the production of Starmerella bombicola yeast compositions.

The present invention relates to a cell culture device (1) for use with an optical microscope (40), comprising a housing (10) that is configured to be placed onto a stage of an optical microscope (40) in front of an objective (41) of the microscope (40), the housing (10) enclosing an internal space (11) of the housing (10), a removable flow chamber (2) enclosing an internal space (20) for accommodating a cell culture (CC) comprising living biological cells, a heater (3) arranged in the internal space (11) of the housing (10) for heating said fluid medium (M) to be guided through the flow chamber (2), a first flow path (P1) arranged in the internal space (11) of the housing (10) for guiding said fluid medium (M) towards the flow chamber (2) via said heater (3), and a second flow path (P2) arranged in the internal space (11) of the housing (2) for guiding said fluid medium (M) away from the flow chamber (2), and a pump (4) for pumping said fluid medium (M) through the first flow path (P1) into the internal space (20) of the flow chamber (2) and through the second flow path (P2) out of the internal space (20) of the flow chamber (2) when the flow chamber (2) is inserted into the internal space (11) of the housing (10).

A system and method of controlling temperature of a medium by refrigerant vaporization, or working gas condensation, or a combination of both, the system including a container, at least one a working gas reservoir having at least one reservoir section that includes a wall with an exterior surface structured to be thermally coupled with a volume of the medium in the container and to provide a volume of medium thermal coverage in the container, a condensation apparatus to provide regulation of working gas condensation in the reservoir, whereby the working gas reservoir forms a vapor space in each of the at least one reservoir section in response to receiving the working gas and to the condensation apparatus regulation of condensation to enable working gas condensation at or near a selected temperature of the volume of medium in the container that is thermally coupled to the respective reservoir section.

The present application relates to a hybrid photobioreactor, which includes a reactor body, wherein the reactor body is equipped with a water tank, a light source device, and a high-pressure rinsing and disinfecting device; the light source device includes transparent casings and LED light strips located within the transparent casings, and the transparent casings are arranged in parallel at intervals within the water tank; the high-pressure rinsing and disinfecting device comprises a plurality of horizontal tubes and a plurality of vertical tubes located under each horizontal tube; a plurality of liquid nozzles are provided on the wall of each vertical tube from top to bottom

Methods and apparatus for culturing microorganisms are described, including culturing in mixotrophic culture conditions. A bioreactor array with multiple culture vessels with independently controllable inputs is used to culture similar cultures of microorganisms in which at least one parameter differs from other culture vessels in the bioreactor array.