Disclosed is a method of artificial intelligence-based robotic pipetting for cell preparation. The method includes training an artificial intelligence/machine learning system (AI/ML system) to classify images of biological specimens. The method includes storing the classified images. The method includes receiving an image object from an imaging system that includes a microscopy system, a camera system configured to receive imaging from the microscopy system, and a lighting system configured to illuminate a biological material. The method includes processing the image object, using the AJ/ML system to determine a presence of a retrievable target cell. The method includes positioning a robotic pipettor at a target physical orientation relative to the retrievable target cell. The method includes confirming the robotic pipettor's location at the target physical orientation, using AI/ML system. The method includes instructing the robotic pipettor to obtain the retrievable target cell from the target physical orientation.

Disclosed is a jacketed, tiered baffle, bioreactor tank comprising an outer cylindrical-shaped jacket and a cylindrical tank having an inner tank surface defining a chamber configured for supporting a flexible bag disposed within the chamber, and an outer tank surface having tiered baffles configured for routing a heat exchange fluid around the entirety of the outer tank surface, the cylindrical tank disposed axially within the outer cylindrical-shaped jacket. The outer cylindrical-shaped jacket is sealed to the cylindrical tank in a manner sufficient to prevent or minimize loss of the heat exchange fluid.

An exposure device for exposing microorganisms to gaseous pollutants and a corresponding in-situ multi-toxicity endpoint detection system are provided. The detection system based on the exposure device disclosed in the present application may perform controlled accumulation of pollutants in air, and implements in-situ, comprehensive, quick, and low-cost assessment of toxicity effects of pollutants in air of a target site. The exposure device and the detection system disclosed in the present application can effectively avoid toxicity distortion caused by complex collection and transfer process, complex chemical reactions in a liquid elution process in conventional toxicity detection methods for gaseous pollutants.

A bioreactor exhaust system includes an exhaust tube, a shut off valve, and a vessel. The exhaust tube includes a level sensor. The vessel includes a sterilant. The shut off valve is responsive to the sterilant reaching the level sensor.

One or more systems for automated preparation of a regenerative epidermal suspension comprises a base unit, a cartridge assembly containing a cup, and a tissue disaggregator. The system may also include related tools. The system executes one or more sequences wherein a first motor actuates the pestle against a tissue sample that is disposed along a screen within the cup while the tissue sample is in contact with, in sequence, an enzyme solution, a first buffer solution, and then a second buffer solution. To transition between fluidic contact stages or steps, the tissue disaggregator engages with and lifts the cup into a raised position within a processing area of the cartridge while a base plate motor rotates the appropriate well into alignment under the cup for each processing step. Once the sequence is complete, a user may draw up a regenerative epidermal suspension through an opening in the cartridge top cover.

A bioreactor exhaust system includes an exhaust tube, a shut off valve, and a vessel. The exhaust tube includes a level sensor. The vessel includes a sterilant. The shut off valve is responsive to the sterilant reaching the level sensor.

Disclosed is a heat exchange module for use in a chemical, pharmaceutical or biological reactor system, the module configured to be disposed in the reactor system having a flexible single use container, and including at least one thermally conductive surface adapted to contact the flexible single use container to facilitate heat transfer, and a fluid circulation path through which a heat exchange fluid can be circulated.

A system and method of controlling temperature of a medium by refrigerant vaporization, or working gas condensation, or a combination of both, the system including a container, at least one a working gas reservoir having at least one reservoir section that includes a wall with an exterior surface structured to be thermally coupled with a volume of the medium in the container and to provide a volume of medium thermal coverage in the container, a condensation apparatus to provide regulation of working gas condensation in the reservoir, whereby the working gas reservoir forms a vapor space in each of the at least one reservoir section in response to receiving the working gas and to the condensation apparatus regulation of condensation to enable working gas condensation at or near a selected temperature of the volume of medium in the container that is thermally coupled to the respective reservoir section.

The invention relates to a process for preparing a liquid oat base or drink from an oat derived material. The process comprises a first enzymation of the oat derived material, heating the oat derived material to terminate the enzymation thereof, and a second enzymation of the oat derived material. The first and/or the second enzymation comprises contacting the oat derived material with starch degrading enzyme, and the first and/or second enzymation further comprises contacting the oat derived material with protein solubilizing enzyme.

The invention also relates to a system for preparing a liquid oat base or drink from an oat derived material.

A method for contamination control when growing yeasts is provided. Bacterial contamination is controlled by using urea as the primary nitrogen source while simultaneously limiting the amount of nickel available to contaminating bacteria. Bacteria require nickel as a cofactor for urease enzymes in order to use urea for growth while yeasts do not require nickel as a cofactor for any enzymes. Nickel is limited by using titanium in plate heat exchangers instead of stainless steel. Ethyl carbamate is limited by using a carbon/nitrogen ratio that consumes all urea during fermentation and by separating co-products after fermentation and before distillation. Yeast recycling is performed by using either single-step or two-step centrifugation, without acid washing. This method enables yeast recycling with sugarcane ethanol and sugar beet ethanol production. This method also enables yeast recycling with corn ethanol and grain ethanol production with coproduct recovery after fermentation and before distillation.