A method of controlling a cell culture process uses hybrid predictive modeling in a model predictive controller. The method includes, for multiple time intervals, obtaining current values of cell culture attributes associated, and generating a control value for a physical input to the cell culture process. Generating the control value includes predicting future values of the cell culture attributes based on the current values, by using one or more data-driven models to predict future values of a first one or more attributes of the cell culture attributes, and using one or more first principle models to predict future values of a second one or more attributes of the cell culture attributes. Generating the control value also includes determining the control value by optimizing an objective function subject to the predicted future values. The method also includes using the control value to control the physical input to the cell culture process.

Methods for determining ethylene concentration in an ethylene oligomerization reactor using an ultrasonic flow meter are described, and these methods are integrated into ethylene oligomerization processes and related oligomerization reactor systems.

Methods of standardizing a fermentation process may include obtaining a fluidic sample, measuring one or more physical parameters of the sample, comparing the measurement of the physical parameter of the material to a baseline value of the physical parameter for the fermentation process, and responsive to a deviation of the measurement of the physical parameter from the baseline value, determining a remediation action based on a correlation between the physical parameter and regulatory genes of a fermentation organism.

A bioreactor including a bioreactor body, wherein the bioreactor body includes a first substrate and an opposing second substrate, a pathway extending through the bioreactor body and being formed by a first channel defined in the first substrate and an opposing second channel defined in the second substrate, a first inlet for introducing a first fluid flow to the first channel, a second inlet for introducing a second fluid flow to the second channel, a first outlet for permitting the first fluid flow to exit the first channel, a second outlet for permitting the second fluid flow to exit the second channel, a membrane disposed in the pathway between the first and second channels and having a plurality of pores sized to selectively capture, in the first channel, a biological source material and to permit biological products to be collected from the bioreactor.

The present disclosure provides an in vitro life culture system, comprising: a culture module used to cultivate a culture, the culture module including at least a culture chamber for holding a culture fluid; a culture fluid provision module used to provide the culture fluid to the culture module; and a fluid output module used to discharge the culture fluid from the culture chamber.

The present invention provides a method of culturing an alga that enables practical realization of low-cost and space-saving production of biofuels and bioenergy by culturing the alga using a reaction tank equipped with a digestive solution tank comprising digestive solution containing high concentration of nutrient salts, a membrane with a pore size of 0.45 .Math.m or less and a culture tank comprising culture solution and the alga, which comprises consuming the nutrient salts by the alga in the culture tank to maintain the difference in concentration the nutrient salts between the digestive solution tank and the culture tank and supplying the nutrient salts contained in the digestive solution through the membrane into the culture solution by the diffusion depending on the concentration difference as well as an alga culture system therefor.

Algae harvesting and cultivating systems and methods for producing high concentrations of algae product with minimal energy. In an embodiment, a dead-end filtration system and method includes at least one tank and a plurality hollow fiber membranes positioned in the at least one tank. An algae medium is pulled through the hollow fiber membranes such that a retentate and a permeate are produced.

A bioreactor includes a plurality of spargers and a plurality of vertical circulation loops. A first vertical circulation loop of the plurality of vertical circulation loops includes a first sparging region and a first return region. The first vertical circulation loop is in liquid communication with one or more other loops of the plurality of vertical circulation loops. The first vertical circulation loop is characterized by an individual loop mass transfer coefficient. A controller is coupled to the plurality of spargers and configured to control the plurality of spargers together such that a cumulative mass transfer coefficient of the plurality of vertical circulation loops is within a threshold of the individual loop mass transfer coefficient associated with the first vertical circulation loop.

An airlift bioreactor includes a body, a first sparging region, a second sparging region, a first sparger configured to sparge a first gas within the first sparging region, a second sparger configured to sparge a second gas within sparging region, and a controller coupled to the first sparger and the second sparger. The controller is configured to control a first flow rate associated with the first sparger and a second flow rate associated with the second sparger.

Control of volume in bioreactors and associated systems is generally described. Feeding and/or sampling strategies can be employed, in some embodiments, such that the working volume within the bioreactor remains substantially constant.