Intravaginal culture (IVC) container for intravaginal fertilization and culture of mammalian, and in particular human, oocytes, featuring an increased volume, and a method of using the same.

The present disclosure relates to use of specialized vessels for formulating and dispensing pharmaceutical formulations. The vessels are optionally able to maintain homeostatic conditions and a homogeneous constitution of pharmaceutical suspensions, while simultaneously dispensing them into single-use containers.

The invention relates to a device for examining a medium (100) inside a bioreactor (200; 201), comprising a sample-taking module (20) for taking a sample of the medium (100). The sample-taking module (20) comprises an uptake region (10; 10a; 10b) that can be arranged to make contact with the medium (100) inside the bioreactor (200; 201). At least two different membranes (15, 16) are positioned on the uptake region (10; 10a; 10b) of said sample-taking module (20), for the purpose of taking a sample of the medium (100).

In an aspect, there is provided a computer-implemented method for approximating product production in a bioreactor. The method comprises: providing a bioreactor containing live cells in a substrate, the bioreactor for cultivating, over a time period, a product derived from or of the cells during a manufacturing process; providing two or more sensors for measuring respective two or more different physical attributes of the substrate during the time period; receiving, at a processor, sensor data from the two or more sensors; and determining via the processor, based on a predetermined or recursive algorithm that correlates the sensor data and the product production by independent consideration of the sensor data of the two or more different physical attributes or by multivariate consideration of the sensor data of the two or more different physical attributes, an approximate amount of the product.

Systems and methods for automated cell culturing are disclosed. In some embodiments, one or more cell culture vessels are fluidly connected with one or more multiport valves and one or more fluid pumps. The fluid pumps may pump various fluids into and out of the cell culture vessels as necessary to support cell growth, routed by the one or more multiport valves. In some embodiments, one or more components may be removable from other components so that some components may be prepared and sterilized independently prior to usage.

A method for studying cell viability and protein aggregation involves establishing a Fabry Perot etalon signal within an optical spectroscopic feature, e.g., in the near infrared region. Protein aggregation and cell viability can be reflected by changes observed in the magnitude of the Fourier Transform peaks observed in the frequency or space domain associated with the contrast of the etalon. In short, the presence of viable cells and protein aggregates can degrade the etalon contrast of an etalon window. In some cases, the concentration of cells and monomeric protein can be measured as well.

The present disclosure provides cell culture chambers for use in automated cell engineering systems, and in particular, cell culture chambers that include improved cell-contacting surfaces. Improved cell-contacting surfaces can include a surface coating that promotes cell growth, adherence, differentiation, maintenance of phenotype, and/or improves transduction; a cell-contacting surface comprising a non-porous, gas-permeable material; as well as other modifications to the cell-contacting surfaces. Cassettes comprising the cell culture chambers are also provided.

The present invention is in the field of large-scale production of cultured cells, providing systems comprising a plurality of scaffolds arranged optionally in a multi-layer configuration and methods of use thereof for production of cells and/or tissue cultures for a variety of uses, including the production of cultured food products, particularly cultured meat.

The present disclosure is directed to systems and methods for sampling and/or controlling the productivity of a bioreactor. The system and methods can include a vessel capable of providing an environment suitable for containing whole broth that can produce the bioproduct, wherein the whole broth contains media and at least one undissolved species, an automated sampling system, a first analytical instrument, and a control system.

The present invention relates to a method for synthesizing an RNA molecule of a given sequence, comprising the step of determining the fraction (1) for each of the four nucleotides G, A, C and U in said RNA molecule, and the step of synthesizing said RNA molecule by in vitro transcription in a sequence-optimized reaction mix, wherein said sequence-optimized reaction mix comprises the four ribonucleoside triphosphates GTP, ATP, CTP and UTP, wherein the fraction (2) of each of the four ribonucleoside triphosphates in the sequence-optimized reaction mix corresponds to the fraction (1) of the respective nucleotide in said RNA molecule, a buffer, a DNA template, and an RNA polymerase.

Further, the present invention relates to a bioreactor (1) for synthesizing RNA molecules of a given sequence, the bioreactor (1) having a reaction module (2) for carrying out in vitro RNA transcription reactions in a sequence-optimized reaction mix, a capture module (3) for temporarily capturing the transcribed RNA molecules, and a control module (4) for controlling the infeed of components of the sequence-optimized reaction mix into the reaction module (2), wherein the reaction module (2) comprises a filtration membrane (21) for separating nucleotides from the reaction mix, and the control of the infeed of components of the sequence-optimized reaction mix by the control module (4) is based on a measured concentration of separated nucleotides.