Systems and methods for producing cultured food products such as cultured meat in a form of meat cut or offal are provided, comprising growing non-human-animal adherent cells on edible scaffold(s) in a cultivation system. The cultivation system typically comprises a plurality of cell culture bioreactors receiving medium at a controlled flow rate adjusted to nourish the non-human-animal adherent cells.

An information processing apparatus acquires input information including at least one of a process condition, a culture medium component, or the number and diameters of cells in cell culture, and estimates a quality of an antibody produced from the cells and a quality of the cells after elapse of a predetermined period from a time point at which the input information is acquired, on the basis of the input information and a trained model which is trained in advance using the input information, the quality of the antibody, and the quality of the cells.

An information processing apparatus estimates a quality of an antibody produced from cells and a quality of the cells on the basis of a culture state of the cells, searches for the culture state of the cells that improves the estimated quality of the antibody and the estimated quality of the cells, and derives process conditions for cell culture in which a culture state of the cells is the searched culture state.

Bioreactor systems and controlled operation of bioreactor systems are disclosed herein. The bioreactor systems can include at least one bioreactor chamber, at least one reservoir, a plurality of sensors, and a fluid circuit. The operational methods disclosed herein are directed towards growing cells or tissue while measuring various parameters, and a controlled operation of the various parameters during the operation of the bioreactor systems. The controlled operation of the parameters includes, for example, cell concentration; a rate of flow; a volume; a pH; a temperature; a level of oxygen; a level of carbon dioxide; a level of bicarbonate ion; nutrient compound; and any combination thereof.

The present invention relates to bioreactor system and method thereof wherein support matrix (2) comprises at last one central shaft and plurality of peripheral shaft being radially surrounds central shaft. Arrays of discs (11) are mounted along the shaft by defining interspatial vicinities between two successive plates. Thus, discs mounted on peripheral shafts are rotated within the interspatial vicinity of discs of central shaft to ensures sufficient mixing and avoid stagnant fluidic zones which is created when discs are mounted closely apart from each other on shafts. Further, plurality of deflector vanes that are axially provided along the length of the central shaft to redirect substantially co-axial direction fluid flow into interior of culture vessel and more specifically towards the central axis. Thus, bioreactor system provides scalable and disposable bioreactor with efficient mixing and homogeneous conditions and thereby supports high density growth and maintenance of cells and other biological material.

Methods and apparatus of bioreactors for therapeutic cells manufacturing are provided herein. In some embodiments, a bioreactor includes: an upper bioreactor reservoir configured to perform multiple cell therapy manufacturing process steps including genetic modification and expansion to a plurality of cells disposed therein, wherein the upper bioreactor reservoir includes a plurality of ports for delivering fluids into and out of the upper bioreactor reservoir; a lower bioreactor compartment configured to hold a suspension comprising a molecular species; and a membrane disposed between the lower bioreactor compartment and the upper bioreactor reservoir, wherein the membrane includes a plurality of micro-straws extending through the membrane and into the upper bioreactor reservoir to transfect the plurality of cells with the molecular species.

A bioreactor system having an expanding volume is provided. The system includes a bioreactor assembly comprising a plurality of bioreactors including at least a first bioreactor and a second bioreactor proximate the first bioreactor. The bioreactor system further includes a first gate between the first bioreactor and the second bioreactor such in a first configuration the gate is closed and the first bioreactor is isolated from the second bioreactor and in a second configuration the gate is open and the first bioreactor is contiguous with the second bioreactor and media and cells form a homogenous mixture within a combined volume formed by the first bioreactor in combination with the second bioreactor. The bioreactor system may further include a microdispenser for each of the plurality of bioreactors for controlling dispensement of media into each of the plurality of bioreactors.

A method of controlling a cell culture process includes, for each time interval of one or more time intervals during the cell culture process, obtaining current values of one or more cell culture attributes associated with a cell culture, predicting one or more future values of a particular cell culture attribute associated with the cell culture, and controlling one or more physical inputs to the cell culture process. Predicting the future value(s) includes applying the current values of the cell culture attribute(s), and an earlier value of at least one of the cell culture attributes, as inputs to a data-driven predictive model using historical data. Controlling the physical input(s) includes applying the future value(s) as inputs to a model predictive controller.

The present disclosure relates to a bioreactor assembly having a housing defining an interior chamber; a lid assembly removably coupled to the housing and enclosing the interior chamber; and a gimbal assembly disposable within the interior chamber. The gimbal assembly includes a cradle configured to hold an organ or organ scaffold and an arm assembly configured to move the cradle between a plurality of positions. The bioreactor assembly also includes an ultrasound imaging unit positioned to capture volumetric and/or spatial data of the organ or organ scaffold.

The invention relates to a method for controlling an enzymatic pre-treatment process, e.g. a mashing process. The method allows for accurate determination of specific sugar molecules as well as the average length of the sugar chains in real-time during e.g. a mashing process. Further information on e.g. the concentration of dissolved protein and free amino acids can also be obtained simultaneously. The method comprises the use of an infrared (IR) spectometer for continuously measuring attenuated total reflectance (ATR) IR spectra of samples obtained during pre-treatment of biomass.