An extra-capillary fluid cycling unit for maintaining and cycling fluid volumes in a cell culture chamber includes a housing and a first flexible reservoir extra-capillary fluid reservoir disposed in the housing. The extra-capillary fluid reservoir is in fluid communication with a cell culture chamber. A second flexible reservoir is also located in the housing, the second flexible reservoir being in fluid communication with a pressure source. A sensor plate is movably disposed in the housing between the extra-capillary reservoir and the second reservoir, wherein the second reservoir is pressurized to move the sensor plate in relation to the extra-capillary reservoir to cause fluid cycling and maintain fluid volumes in the cell growth chamber.

This disclosure relates to reaction container systems providing for headspace-based condensation, coalescing devices, and other features. In some embodiments, this disclosure provides systems that reduce the relative humidity (RH) of an exhaust gas prior to or concurrent with its expulsion from the system through an exhaust filter.

A gas concentration regulator for use in culture of bacteria, including: (a) an ascorbic acid component; (b) a transition metal catalyst; (c) activated carbon; (d) an alkali metal carbonate or an alkaline earth metal hydroxide; and (e) water, wherein the activated carbon is impregnated with the ascorbic acid component and the water, and an average pore diameter of the activated carbon is 2.0 nm or more.

There is provided a bioreactor including: a chamber unit having an accommodating space therein; an upper fixing member and a lower fixing member that are disposed at respective planes perpendicular to a central axis of the chamber unit inside the chamber unit and are movable in a central-axis direction of the chamber unit; and a filling layer that is formed in a space between the upper fixing member and the lower fixing member inside the chamber unit and contains a plurality of carriers.

The present invention is related to the field of microfluidics and compound distribution within microfluidic devices and their associated systems. In one embodiment, present invention aims to solve the problem of molecule and compound absorbency into the materials making up laboratory equipment, microfluidic devices and their related infrastructure, without unduly restricting gas transport within microfluidic devices.

Embodiments of the present disclosure provide a biological sample imaging device, including an incubator with an observation window provided on one side; a biological sample cultivation module provided inside the incubator; an imaging module provided outside the incubator for collecting images of a biological sample cultured in the biological sample cultivation module through the observation window, so that the growth process can be dynamically captured, and the environment in the incubator can be conveniently controlled to adapt to various scientific research scenarios. In addition, the isolation inside and outside the incubator can reduce the interference of the imaging module on the biological sample.

An analytical instrument having a sensing system, a stage adapted to receive a sample carrier, a motion actuator assembly for positioning the sensing system and/or the stage, a temperature control element, and a signal processing module are disclosed. Methods of analyzing cell samples with the analytical instrument are also disclosed.

A method and apparatus of aseptic processing and production of cells in a non-sterile enclosure apparatus without chemical biocides is disclosed, by controlling the level of humidity throughout the enclosure to 25% relative humidity (RH) or less, and preferably 20% or 15% or less RH. In addition, the temperature is controlled to 37° C., and consistent gas flow is maintained the enclosure. Colony forming units from microbial contamination detected by environmental monitoring within the enclosure are significantly reduced in this method.

A bioprocess device assembly includes at least a first bioreactor and a second bioreactor, and a transfer system for transferring a certain volume of a medium contained in the first bioreactor into the second bioreactor. A biomass sensor is associated to the first bioreactor. A control unit is configured for receiving measurement data from the biomass sensor and controlling the transfer system. An inoculation method makes use of such a device assembly wherein the biomass sensor measures at least one parameter of biomass contained in the first bioreactor; the biomass sensor sends measurement data to the control unit; and the control unit evaluates the measurement data and, based on an evaluation of the measurement data decides whether the viable cell density is sufficient for inoculation, determines a necessary inoculation volume, and controls the transfer system to transfer the determined inoculation volume from the first bioreactor into the second bioreactor.

Control of humidity in chemical reactors, and associated systems and methods, are generally described. In certain embodiments, the humidity within gas transport conduits and chambers can be controlled to inhibit unwanted condensation within gas transport pathways. By inhibiting condensation within gas transport pathways, clogging of such pathways can be limited (or eliminated) such that transport of gas can be more easily and controllably achieved. In addition, strategies for purging condensed liquid from chemical reactor systems are also described.