Cell culture systems and methods provide improved immunotherapeutic product manufacturing with greater scalability, flexibility, and automation. Cell culture systems are configured with interchangeable cartridges, allowing versatility and scalability. Systems are configured to have multiple connected cell culture chambers, which allows parallel processing of different types of cells. Gas-impermeable cell culture chambers and methods for generating cells in closed systems prevent contamination and user error. Methods for recycling cell culture medium provide additional efficiencies.

A bioreactor sensing system is presented. The bioreactor sensing system comprises a bioreaction vessel. The bioreaction vessel comprises an aperture configured to provide an interface for monitoring contents within the bioreaction vessel. The bioreaction vessel also comprises a port coupled to the bioreaction vessel proximate the aperture. The bioreactor sensing system also comprises a sensing device disposed at least partially within the port such that a sensing element is exposed to the contents. The bioreactor sensing system also comprises an external seal configured to be applied over a portion of the sensing device and a portion of the port.

The present disclosure provides a fully enclosed cell culture system, including at least an incubator, a bioreactor, a gas flow assembly, a liquid flow assembly, a temperature adjustment assembly, and a central controller. The system realizes a thermostatic incubation environment. Cells are incubated by perfusion, and a fully enclosed integrated process from cell activation, infection and amplification to finished product harvesting is achieved. By axially stirring the cells and an incubation liquid in a tank body, a radial shear force is reduced, which effectively protects the cells and improves the cell yield. In the system of the present disclosure, gases are separately introduced, ensuring that the content of each gas component is stable in the incubation process. The cell incubation is not directly affected by changes of outside gases in the cell incubation process, which improves culture efficiency.

The present disclosure relates to methods of preparing personalized blood vessels, useful for transplantation with improved host compatibility and reduced susceptibility to thrombosis. Also provided are personalized blood vessels produced by the methods and use thereof in surgery.

The invention comprises one or more gas volume fraction measurement devices operatively connected to one or more controllers and one or more deaeration mechanisms which receive control signals from said one or more controllers and perform an act on the system, such as by controlling a level of deaeration chemistry into some portion of the fermentation system. In one embodiment, the deaeration mechanism is an antifoam feed pump which pumps antifoam chemistry into a feed line of the fermenter in response to the measured gas volume fraction in the fermenter's recirculation loop, in an amount determined by the controller to be effective to reduce foaming and lower column height in the fermenter.

The present disclosure provides a bioreactor, which includes a tank configured to contain a mixture of animal cells and a liquid; and a first ventilation device arranged outside the tank and including a first gas distributor, the first gas distributor being configured to transmit a gas to the liquid separated from the animal cells. The bioreactor further includes a dialysis component, which is arranged outside the tank and comprises a dialysis filter. The bioreactor further includes a second ventilation device arranged in the tank and configured to transmit the gas to the mixture in the tank.

A portable incubator system integrated to a mobile phone providing a real-time tracking of samples and data flow is provided. The portable incubator system allowing cells to be cultured, reproduced and characterized in real-time without a need for a commercial incubator and a microscope-camera system installed within the portable incubator system.

The present invention relates to an automatic culture device using a single-use closed system flow path for culturing a cell or a tissue, and realizes reduction in manufacturing cost and high integration property of a device. A cell culture system including an automatic culture device and an information processing device. The automatic culture device includes a plurality of types of closed system flow paths possible to be installed and removed, and a plurality of culture devices. The information processing device includes: an input device configured to receive, as an input, at least one piece of data selected from a group consisting of data of an identifier of a patient, data related to a transplantation method, data related to a type of cell, data related to the required number of cells, and data related to a treatment plan; an arithmetic device configured to select, based on the input data, from options of a cell culture method, the culture devices, and the closed system flow paths, the cell culture method, the culture device, and the closed system flow path to be used; and an output device configured to output a number of the closed system flow path to be used and a number of the culture device to be used.

An incubation cassette for reducing liquid evaporation from wells of a microplate with a frame for receiving a microplate with wells, the frame having a central first opening surrounded by an inner wall, the dimensions are designed for inserting a microplate, and the frame having an outer wall that runs essentially parallel to the inner wall and which is attached to the inner wall connects so that a liquid reservoir surrounding the first central opening for receiving a liquid is formed by the two walls and the intermediate base is disclosed. The incubation cassette also includes a float provided in the liquid reservoir that can be brought into fluid contact with the liquid held in the liquid reservoir in such a way that the float is in relation to the liquid level of the liquid in the liquid reservoir absorbed liquid experiences buoyancy.

A bioprocessing installation comprising a reservoir tank, one or more fluid lines, one or more receiving containers and an electronic control unit. The fluid line fluidically connects the reservoir tank to a receiving container. The liquid biological medium can be transferred from the reservoir tank through the fluid line into the at least one receiving container. The bioprocessing installation comprises a sensor arrangement with at least one gas bubble sensor, which sensor arrangement is configured to detect one or more gas bubbles or foam or separated phases in the liquid biological medium by a gas bubble sensor and to generate one or more corresponding sensor signals, and the electronic control unit is configured to monitor the transfer of the liquid biological medium from the reservoir tank in the direction of the at least one receiving container based on the sensor signals generated by the sensor arrangement.