A cell-culture bioreactor operative to provide real-time reactor management in the fields of stir control, sparge control, and heat management responsively to wireless sensor feedback to optimize operating conditions to maximize cell-culture yield; and a bioreactor comprising: a vessel, having an open top; a headplate, configured to seal the vessel's open top; and a stirring device, comprising at least two independent stirring elements; wherein each of the stirring elements is configured to independently stir media accommodated within the vessel.

A method for monitoring a biotechnological process, wherein starting materials are converted into products via a biomass and important process parameters for monitoring are identified during the process, where during the process, a current concentration of the biomass utilized in the process is recurrently estimated, current measurement values of measurable process parameters are then recurrently determined on a recurring basis and current values for additional process parameters are identified therefrom, where the current measurement values of the measurable process parameters and the current determined values of the additional process parameters are based on the respective temporally correlating concentration of biomass and where, from a combination of the current concentration of biomass and the current measurement values of the measurable process parameters and the identified current values of the additional process parameters, current, cell-specific metabolic indicators are then derived which are then used in conjunction with a deterministic process model.

The present invention can provide an incubator having not only a favorable cell culture function but also an autonomous decontamination function that saves the need for connection to an external decontamination device.

The incubator provided with a culture vessel, a circulating means, a temperature-regulating means, and a gas supplying means for supplying a decontamination gas or a humidifying water vapor. The gas supplying means includes a compressed gas generating means, a decontamination solution supplying means, a water supplying means, a mixed gas/liquid regulator, and a vaporizer for generating a decontamination gas or a water vapor. The gas supplying means acts as a decontamination means for supplying the decontamination gas to the air circulating in the circulation passage during an autonomous decontamination before cells are cultured, and the gas supplying means acts as a humidifying means for humidifying the air circulating in the circulation passage when cells are cultured after the autonomous decontamination.

The invention relates to a system (100) for monitoring deviations of a state of a cell culture in a bioreactor (104, 106) from a reference state of a cell culture in a reference bioreactor (102). The bioreactor comprises the same medium (M1) as the reference bioreactor. The system comprises: •—a storage medium (114) comprising: •a PACO-reference profile (116) indicative of a deviation of a CO2 off gas rate (ACO.sub.R-M-.sub.ti) measured in the reference bioreactor from a predicted CO2 off gas rate (ACO.sub.R-EXP-ti) of the reference bioreactor; •a data object comprising a medium-specific relation (136) between the pH value of the medium (M1) and a respective fraction of CO2 gas in a gas volume when said medium is in pH-CO2 equilibrium state with said gas volume and lacks the cell culture; •—an interface (128) for receiving (212) a current CO2 off gas rate (ACO.sub.Bi-M-ti, ACO.sub.B2-M-.sub.t i) and a current pH value (pH.sub.Bi-ti) of the medium of the bioreactor (104, 106); •—a comparison unit (130) configured for computing (214, 216): •a PACO value (PACO.sub.B1-tir PACO.sub.Bi-ti) the PACO-value being indicative of a deviation of a CO2 off gas rate (ACO.sub.Bi-M-ti, ACO.sub.B2-M-.sub.ti) measured in the bioreactor from a predicted CO2 off gas rate (ACO.sub.B1-EXP-ti, ACO.sub.B2-E xp-.sub.t i). a difference between the computed PACO value (PACO.sub.Bi-ti, PACO.sub.B2-ti) and a respective reference PACO value (PACO.sub.R-ti) in the PACO-reference profile (116).

Embodiments of the present disclosure involve systems and methods that inhibit the production of lactic acid during propagation of yeast and/or during hydrolysis of cellulose by including a sufficient amount of dissolved oxygen.

The present disclosure relates to methods of preparing personalized blood vessels, useful for transplantation with improved host compatibility and reduced susceptibility to thrombosis. Also provided are personalized blood vessels produced by the methods and use thereof in surgery.

The devices, methods and systems are described for providing controlled amounts of gas, gas pressure and vacuum to microfluidic devices the culturing of cells under flow conditions. The devices, methods, and systems contemplated here may also be used to control the environment surrounding the microfluidic devices; offer user control over experiments comprising microfluidic devices, such as the ability to directly or remotely control experiment conditions; and comprise information aggregation and transmission, such that experimental data may be collected, stored, aggregated and transmitted to users.

Provided herein are gas mixing systems, comprising a gas inlet for receiving two or more gases and a mixing chamber with a static mixer for mixing the gases. Preferred mixing chambers further comprise a reduced pressure compartment downstream of the static mixer that is in fluid communication with the gas inlet. A gas outlet is in fluid communication with the mixing chamber, and one or more sensors are coupled to the reduced pressure compartment and are configured to continuously sense various parameters such as barometric pressure and the percentage of oxygen in the gas mixture moving through the mixing device. Most typically, the readings of the sensor are pre-compensated for temperature, pressure, and humidity. Also provided herein are methods for using the same.

An incubator for cell and tissue culture under controlled atmospheric conditions has a primary air flow control device that forms a primary, preferably laminar flow, air veil across an opening that allows access to the cells or tissue cultures disposed within the incubator. Preferably, most if not all of the air in the primary (laminar flow) air veil is recirculated, and a secondary air flow control device is used that forms a secondary, preferably laminar flow, air veil between the primary (laminar flow) air veil and a user of the incubator.

A gas concentration regulator for use in culture of anaerobic bacteria, including: (a) dehydroascorbic acid; (c) a transition metal catalyst; (d) activated carbon; (e) at least one selected from the group consisting of an alkali metal carbonate, an alkali metal hydroxide, and an alkaline earth metal hydroxide; and (f) water.