Techniques related to the detection or identification of biological cells or substances.

A culturing assistance device includes an image acquisition unit configured to acquire a captured image of cells during culturing at determined times, a storage control unit configured to store the captured image acquired by the image acquisition unit and event information indicating an event related to culturing of the cells, and a learning unit configured to learn a relationship between the stored captured image and the stored event information.

Embodiments described herein involve determining an area of interest on a growth media. An overall brightness control value for a plurality of illumination sources configured to illuminate the growth media is calculated. The overall brightness control value generating at least one image that substantially matches a target intensity at the area of interest. An individual brightness value for each illumination source of the plurality of illumination sources is calculated by individually adjusting a brightness of each illumination source to generate at least one image that substantially matches the target intensity in each respective illumination source's area of influence. A calibrated brightness value for each illumination source is determined based on an image intensity with each illumination source turned on at the respective individual brightness value and an intensity that each illumination source generates within each respective area of influence when turned on alone.

Methods for identifying compounds that modulate cellular responses stimulated by IgE, which include providing an impedance-based system that monitors cell-substrate impedance of cells on a substrate; introducing cells to the substrate of the system; adding at least one test compound and IgE to the cells, wherein the at least one test compound is suspected of modulating cell responses stimulated by the IgE; adding an antigen to the cells; monitoring the cell-substrate impedance of cells on the substrate; and analyzing the cell-substrate impedance to evaluate whether the at least one test compound alters a cellular response to stimulation with the IgE.

The monitoring and control of bioprocesses is provided. The present disclosure provides the ability to generate generic calibration models, independent of cell line, using inline Raman probes to monitor changes in glucose, lactate, glutamate, ammonium, viable cell concentration (VCC), total cell concentration (TCC) and product concentration. Calibration models were developed from cell culture using two different CHOK1SV GS-KO™ cell lines producing different monoclonal antibodies (mAbs). Developed predictive models, qualified using an independent CHOK1SV GS-KO™ cell line not used in calibration, measured changes in glucose, lactate, ammonium, VCC, and TCC with minor prediction errors over the course of cell culture with minimal cell line dependence. The development of these generic models allows the application of spectroscopic PAT techniques in a clinical manufacturing environment, where processes are typically run once or twice in GMP manufacturing based on a common platform process.

The present invention relates to a device and a method for the analysis of cultured cells, comprising a plurality of culture vessels, optical sensors and a detection system with detectors for determining a physico-chemical parameter in a culture vessel and for imaging the morphology of cells in a vessel. The device comprises a microscope adapted to be moved and having an optical path provided with switching means to switch between an optical path for determining a physico-chemical parameter and an optical path for imaging the morphology of cells.

The present disclosure relates to a system for immune therapy, the system comprising a sample processing module configured to obtain whole blood from a subject; a cell incubation module configured to activate blood cells of the whole blood and/or introduce a vector into the blood cells of the whole blood; and a cell infusion module configured to infuse at least a portion of the whole blood to the subject, wherein the blood cells comprise CD3+ cells, NK cells, myeloid cells, and neutrophils.

The present disclosure relates to a scalable experimental workflow that uses a culture system to maintain a steady state in a biological system, and techniques for identifying values for parameters in a in silico model based on experimental data obtained from the biological system. Particularly, aspects of the present disclosure are directed to obtaining measurement data for one or more characteristics of a biological system developed in a culture system, where the measurement data is indicative of each of the one or more characteristics at a physiological steady state where growth of the biological system is occurring at a substantially constant growth rate, determining a value for a parameter of a model of the biological system based on an growth formula, the measurement data, and the substantially constant growth rate, and parametrizing the model with at least the value determined for the parameter.

A apparatus for detecting cells or particles in a fluid container includes a dispenser configured to dispense at least one cell or at least one particle into a defined sub-volume of a fluid with which the fluid container is at least partially filled, and a detection apparatus configured to, in a time-coordinated manner with dispensing the at least one cell or the at least one particle by the dispenser, perform a detection in the defined sub-volume and/or in one or several sub-volumes underneath the defined sub-volume in order to sense the at least one cell or the at least one particle when entering the fluid or immediately after entering the fluid.

A 4D-perfused tumoroid-on-a-chip platform used in personalized cancer treatment. The platform includes a plate with a plurality of bottomless wells that resides atop a microfluidic channel layer, which in turn resides atop a surface acoustic wave (SAW) based sensor layer that is capable of measuring potential pH values of fluids disposed within the platform. The microfluidic channel layer includes a plurality of bioreactors, with each bioreactor including an inlet well, a culture well, and an outlet well. The inlet well, culture well, and outlet well form a closed system via fluid conduits spanning from the inlet well to the culture well, as well as from the culture well to the outlet well. Due to the fluid flow from the plate to the chip, and from the inlet well to the outlet well on the chip through the culture well, target cell (tumoroid) growth is promoted within the culture well.