Methods for identifying compounds that modulate cellular responses stimulated by IgE, which include providing an impedance-based system that monitors cell-substrate impedance of cells on a substrate; introducing cells to the substrate of the system; adding at least one test compound and IgE to the cells, wherein the at least one test compound is suspected of modulating cell responses stimulated by the IgE; adding an antigen to the cells; monitoring the cell-substrate impedance of cells on the substrate; and analyzing the cell-substrate impedance to evaluate whether the at least one test compound alters a cellular response to stimulation with the IgE.

Systems and methods for rapid determination of microorganism growth and antimicrobial agent susceptibility and/or resistance are disclosed.

A method for monitoring a biotechnological process, wherein starting materials are converted into products via a biomass and important process parameters for monitoring are identified during the process, where during the process, a current concentration of the biomass utilized in the process is recurrently estimated, current measurement values of measurable process parameters are then recurrently determined on a recurring basis and current values for additional process parameters are identified therefrom, where the current measurement values of the measurable process parameters and the current determined values of the additional process parameters are based on the respective temporally correlating concentration of biomass and where, from a combination of the current concentration of biomass and the current measurement values of the measurable process parameters and the identified current values of the additional process parameters, current, cell-specific metabolic indicators are then derived which are then used in conjunction with a deterministic process model.

The present invention provides a cell treatment apparatus that can improve the operating rates of an observation unit and a laser irradiation unit in the case of treating a plurality of cell culture vessels. The cell treatment apparatus of the present invention includes: a cell treatment chamber in which cells in a cell culture vessel are treated; an observation unit that can observe the cells in the cell culture vessel; a laser irradiation unit that can irradiate the cells with lasers; a first moving unit that can move the observation unit; and a second moving unit that can move the laser irradiation unit, wherein the cell treatment chamber includes a plurality of regions in which the cells can be treated, each region includes a first cell culture vessel placement unit and a second cell culture vessel placement unit, in the first cell culture vessel placement unit, the cells in the cell culture vessel are observed by the observation unit, in the second cell culture vessel placement unit, the cells in the cell culture vessel are irradiated with lasers by the laser irradiation unit, the observation unit is moved by the first moving unit in a state of being able to observe the cells in the cell culture vessel in the first cell culture vessel placement unit of each region, and the laser irradiation unit is moved by the second moving unit in a state of being able to irradiate the cells in the cell culture vessel in the second cell culture vessel placement unit of each region with lasers.

Provided is a cell analysis device including: a display unit; a storage unit; an analysis result input receiving unit to receive inputs of culture conditions and measured values of items obtained by analyzing a cell cultured under each condition, and store them in the storage unit associating the measured values with a corresponding one culture condition; a culture condition designation input receiving unit to receive input of designation of the culture condition; a correlation evaluation unit to read out measured values of items on the cell cultured under the designated culture condition from the storage unit, and evaluate a magnitude of correlation between the measured values of two items for all combinations of two of the items; and a correlation item presentation unit to display, on the display unit, a predetermined number of combinations extracted from among all the combinations based on an evaluation result by the correlation evaluation unit.

An information processing device detects a cell candidate region for determining a unity of a cell from a vessel image obtained by imaging a vessel in which the cell is seeded and includes at least one processor. The processor performs an acquisition process of acquiring the vessel image, performs a detection process of detecting a cell region including the cell and a cell-like region including an object similar to the cell as the cell candidate regions from the acquired vessel image, and an output process of outputting information indicating the detected cell candidate regions.

Methods for monitoring, controlling and simulating a bioprocess comprising a cell culture in a bioreactor are provided. The methods comprise obtaining values of one or more process conditions for the bioprocess at one or more maturities, and determining the specific transport rates of one or more metabolites in the cell culture using the values obtained as input to a machine learning model trained to predict the specific transport rates of the one or more metabolites at a latest maturity of the one or more maturities or a later maturity based at least in part on the values of one or more process conditions for the bioprocess at the one or more preceding maturities. The methods further comprise predicting one or more features of the bioprocess based at least in part on the determined specific transport rates. Systems, computer readable media and methods for providing tools to implement such methods are also provided.

A liquid filtration system according to the present invention comprises a bioreactor; a filter configured to filter liquid passing therethrough; a perfusion pump configured to move liquid between the bioreactor and the filter; a liquid line providing fluidic communication between the bioreactor, perfusion pump and filter; and a bleed outlet provided on the liquid line, the bleed outlet configured to provide means to remove liquid selectively from the system under the action of the perfusion pump. With the liquid filtration system according to the present invention, both the filtering and bleed functions may be performed under the action of a single pump, thereby significantly reducing the complexity and cost of the components required.

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

Even for the case where cells such as human epidermal keratinocytes form a dense colony, or the case where cell contours are indefinite, each of the cells is automatically tracked with high precision, and behavior of each cell is analyzed with good precision. There is provided a method for analyzing behavior of a cell, which comprises a detection step of detecting positions of a plurality of cells for every frame of time-lapse images, while determining whether a candidate region extracted from the frame is a cell region by using a dictionary containing image data of cell nuclei; and a tracking step of tracking each cell by using a state space model using position of a most adjacent cell within a predetermined distance from a predicted position as observation data. When any cell is not found within a certain distance from the predicted position, data are considered missing.