The invention provides a method for producing an oil composition (100) from biomass (200), wherein the method comprises: a preparation stage (11) comprising providing a growth medium (300), wherein the growth medium (300) comprises the biomass (200), wherein the growth medium (300) comprises 1-10 wt % dry matter, 0.02-30 wt % of a carbon source, 0.01-5 wt % of a nitrogen source, 0.00001-0.05 wt. % of a magnesium source, and wherein the growth medium (300) has a pH selected from the range of 3.0-8.0, wherein the biomass (200) comprises a non-pasteurized biomass (220); a fermentation stage (12) comprising (a) inoculating the growth medium (300) with a yeast cell (400), thereby providing an cell suspension (500), and (b) controlling a temperature of the cell suspension (500) in the range of 10-40 C., wherein the yeast cell (400) is selected from the species Yarrowia lipolytica, and Cutaneotrichosporon oleaginous, and wherein the fermentation stage is continued until the cell suspension (500) reaches a cell density selected from the range of 1E8-1E10 cells/ml, wherein the pH of the cell suspension (500) is not controlled during the fermentation stage (12); an oil procurement stage (15) comprising separating a yeast oil (110) from the cell suspension (500) to provide the oil composition (100), wherein the oil procurement stage comprises mechanically lysing the cell suspension (500) to provide a lysed cell suspension (600), wherein the mechanical lysing (610) comprises one or more of screw-pressing (630), bead beating, French pressing and homogenizing.

The present invention relates to a method for preparing a microbial cell product, said method comprising: providing an aqueous suspension comprising microbial cells; subjecting said suspension to mechanical cell disintegration at a temperature in the range of 15-35 C., preferably at a pH value in the range of 9-11, to obtain an aqueous suspension comprising disintegrated microbial cells; separating the suspension to provide an extract enriched in small cell fragments, and an extract enriched in large cell fragments; and combining at least a portion of each extract, to provide said microbial cell product. The invention further relates to a microbial cell product obtained or obtainable with said method. The invention further relates to the use of said product in foodstuffs, for example as a substitute for egg-white; in animal foodstuffs; and/or in cosmetic formulations.

The present invention relates to a method for preparing a microbial cell product, said method comprising: providing an aqueous suspension comprising microbial cells; subjecting said suspension to mechanical cell disintegration at a temperature in the range of 15-35 C., preferably at a pH value in the range of 9-11, to obtain an aqueous suspension comprising disintegrated microbial cells; separating the suspension to provide an extract enriched in small cell fragments, and an extract enriched in large cell fragments; and combining at least a portion of each extract, to provide said microbial cell product. The invention further relates to a microbial cell product obtained or obtainable with said method. The invention further relates to the use of said product in foodstuffs, for example as a substitute for egg-white; in animal foodstuffs; and/or in cosmetic formulations.

A method and system for increasing the purity of protein from a system that produces protein, such as a high protein meal, from dry milling of grains including, for example, corn and wheat. The purity of the high protein meal, for example, can be increased by back end enzyme treatment of yeast, which has been separated along with protein from whole stillage. The enzyme(s) can breakdown the yeast into its yeast components that can be removed from the protein to provide for an increased purity of a resulting high protein product.